Isolation of Chlamydia psittaci ovis in sheep thyroid cell culture

1979 ◽  
Vol 104 (3) ◽  
pp. 55-56 ◽  
Author(s):  
G. Robinson ◽  
I. Anderson
Keyword(s):  
Cell Culture ◽  
Chlamydia Psittaci ◽  
Thyroid Cell ◽  
Thyroid Cell Culture

scholarly journals Thyrotropin modifies the synthesis of actin and other proteins during thyroid cell culture

1985 ◽  
Vol 147 (2) ◽  
pp. 263-272 ◽  
Author(s):  
Heloisa PASSAREIRO ◽  
Pierre P. ROGER ◽  
Francoise LAMY ◽  
Raymond LECOCQ ◽  
Jacques E. DUMONT ◽  
...  
Keyword(s):  
Cell Culture ◽  
Thyroid Cell ◽  
Thyroid Cell Culture

Demonstration of growth in porcine thyroid cell culture

Biochimie ◽  
1979 ◽  
Vol 61 (8) ◽  
pp. 923-930 ◽  
Author(s):  
Guy Fayet ◽  
Sonia Hovsepian
Keyword(s):  
Cell Culture ◽  
Thyroid Cell ◽  
Thyroid Cell Culture

Maintenance of a differentiated thyroid-cell culture system

1981 ◽  
Vol 9 (4) ◽  
pp. 305-305
Author(s):  
ANN MURPHY ◽  
CARMEL MOTHERSILL ◽  
M. K. O'CONNOR ◽  
J. F. MALONE ◽  
J. K. TAAFFE
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Thyroid Cell ◽  
Cell Culture System ◽  
Thyroid Cell Culture

scholarly journals Normal Thyroid Structure and Function in Rhophilin 2-Deficient Mice

2005 ◽  
Vol 25 (7) ◽  
pp. 2846-2852 ◽  
Author(s):  
Jens Behrends ◽  
Serge Clément ◽  
Bernard Pajak ◽  
Viviane Pohl ◽  
Carine Maenhaut ◽  
...  
Keyword(s):  
Rho Gtpase ◽  
Clinical Signs ◽  
Structure And Function ◽  
Secretory Process ◽  
Thyroid Cell ◽  
Thyroid Cells ◽  
Thyroid Cell Culture ◽  
And Function ◽  
Deficient Mice ◽  
Stimulation Of

ABSTRACT Rhophilin 2 is a Rho GTPase binding protein initially isolated by differential screening of a chronically thyrotropin (TSH)-stimulated dog thyroid cDNA library. In thyroid cell culture, expression of rhophilin 2 mRNA and protein is enhanced following TSH stimulation of the cyclic AMP (cAMP) transduction cascade. Yeast two-hybrid screening and coimmunoprecipitation have revealed that the GTP-bound form of RhoB and components of the cytoskeleton are protein partners of rhophilin 2. These results led us to suggest that rhophilin 2 could play an important role downstream of RhoB in the control of endocytosis during the thyroid secretory process which follows stimulation of the TSH/cAMP pathway. To validate this hypothesis, we generated rhophilin 2-deficient mice and analyzed their thyroid structure and function. Mice lacking rhophilin 2 develop normally, have normal life spans, and are fertile. They have no visible goiter and no obvious clinical signs of hyper- or hypothyroidism. The morphology of thyroid cells and follicles in these mice were normal, as were the different biological tests performed to investigate thyroid function. Our results indicate that rhophilin 2 does not play an essential role in thyroid physiology.

Effect of glucocorticoids and oestrogen on interleukin-6 production by human thyrocytes from patients with Graves' disease and toxic multinodular goitre and from HTori3 cells

1997 ◽  
pp. 429-432 ◽  
Author(s):  
TH Jones ◽  
RL Kennedy ◽  
SK Justice ◽  
R Davies
Keyword(s):  
Interleukin 6 ◽  
Interleukin 1 ◽  
Antithyroid Drugs ◽  
Graves Disease ◽  
Thyroid Cell ◽  
Multinodular Goitre ◽  
Necrosis Factor Alpha ◽  
Serum Free ◽  
Thyroid Cell Culture ◽  
Serum Free Conditions

Interleukin-6 (IL-6) is a cytokine released by thyrocytes and is involved in disease processes such as autoimmune thyroid disease. The secretion of IL-6 can be stimulated by interleukin-1 (IL-1), tumour necrosis factor-alpha (TNF), serum, TSH and agents which increase intracellular cyclic AMP levels. Antithyroid drugs such as methimazole inhibit IL-6 production by thyrocytes but the effects of glucocorticoids and oestrogen have not been investigated. The effects of dexamethasone and 17 beta-oestradiol on IL-1-, TNF-, TSH-, forskolin- and phorbol 12-myristate 13-acetate (PMA)-stimulated IL-6 release in serum-free conditions were studied in human thyrocytes derived from patients with Graves' disease and toxic multinodular goitres, and in the immortalised human thyrocyte cell line, HTori3. Dexamethasone inhibited IL-6 production under stimulated conditions. In serum-free conditions, no basal release of IL-6 was assayable. In all but one of the primary thyroid cultures, TSH did not stimulate IL-6 release above the lower detectable limit of the assay. In Graves' and multinodular goitre thyrocytes, inhibition of IL-1 (100 U/ml)-stimulated IL-6 release by dexamethasone (100 nmol/l) was 62.51% +/- 10.43 (S.E.M.), and in HTori3 cells it was 78.35% +/- 3.9. The degree of IL-1 stimulation of IL-6 release and inhibition by dexamethasone was not significantly different in thyrocytes derived from either Graves' or multinodular glands. 17 beta-Oestradiol had no effect on IL-1-stimulated IL-6 release in either primary thyroid cell culture or in HTori3 cells.

Chlamydia Psittaci in a Koala (Phascolarctos Cinereus) Population in South-East Queensland.

1994 ◽  
Vol 21 (1) ◽  
pp. 41 ◽  
Author(s):  
NA White ◽  
P Timms
Keyword(s):  
Cell Culture ◽  
False Negative ◽  
Clinical Signs ◽  
Chlamydia Psittaci ◽  
Rural South ◽  
Phascolarctos Cinereus ◽  
Urogenital Infection ◽  
Urogenital Infections ◽  
Overt Disease

Clinical signs are useful in determining the level of overt disease. However, neither the complement fixation test, nor the presence of clinical signs of disease are appropriate measures for the detection of Chlamydia psittaci in koalas because of false negative rates of 43 and 57%, respectively. Infection due to C. psittaci was most accurately determined in a population of koalas in rural south-east Queensland by in vitro cell culture of samples from ocular and urogenital sites. Prevalence of infection ranged from 39 to 61% with no evidence of a trend with time. Females had more urogenital and fewer concurrent ocular and urogenital infections than males. Parous females (n = 17) were free of disease and only one was recorded with urogenital infection (cell culture). In non-parous females (n = 16), 6 showed clinical signs of urogenital disease and a further 3 were infected (cell culture).

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