Fertility of bovine semen frozen in four diluents for use in artificial insemination

1977 ◽  
Vol 101 (9) ◽  
pp. 171-171 ◽  
Author(s):  
J. Foulkes ◽  
R. Goodey ◽  
D. Stewart
Keyword(s):  
Artificial Insemination ◽  
Bovine Semen

scholarly journals Influence of breed, season and age on quality bovine semen used for artificial insemination

2017 ◽  
Vol 8 (6) ◽  
pp. 72-78 ◽  
Author(s):  
Claire D’Andre Hirwa ◽  
Donald Rugira Kugonza ◽  
Elyse Amahoro ◽  
Claire Ingabire ◽  
Vincent Niyiragira ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Bovine Semen

12 QUANTIFICATION OF REACTIVE OXYGEN SPECIES IN BOVINE SEMEN WITH DIFFERENT SEMINAL QUALITY AND WITH EXTENDER CONTAINING ANTIOXIDANT EVALUATED BY ARTIFICIAL INSEMINATION

2010 ◽  
Vol 22 (1) ◽  
pp. 164
Author(s):  
J. C. Borges ◽  
M. R. Silva ◽  
A. C. Tedesco ◽  
D. S. Costa ◽  
C. R. Esper ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Artificial Insemination ◽  
Poor Quality ◽  
Pregnancy Rates ◽  
Ros Production ◽  
Oxygen Species ◽  
High Quality ◽  
Bovine Semen ◽  
Major Defect

The aim this study was to evaluate the effects on artificial insemination (AI) of minimizing oxidative stress by adding an antioxidant into the sperm microenvironment. For that purpose, semen of bulls with different seminal quality was evaluated in nature and after addition of extender with and without antioxidant, followed by freezing and thawing, with respect to thiobarbituric acid reactive substances (TBARS) concentration, total antioxidant capacity (TAC), and AI success. TBARS is an indicator of lipid peroxidation and TAC measures free radicals. Twelve ejaculates from 5 bulls were treated with Tris egg yolk extender (CE) and in half of each ejaculate the extender was supplemented with 200 μL of Trolox (AE). Three of these 5 bulls had high-quality semen (mean 11.9 ± 5.9 total defect, 7.7 ± 3.1 major defect, and 4.2 ± 3.9 minor defect) and 2 animals had poor-quality sperm (mean 31.9 ± 9.7 total defect, 26.3 ± 8.5 major defect, and 5.6 ± 5.0 minor defect) (P < 0.05). For AI, 300 Nelore heifers were divided in 10 groups and the females in each group were inseminated with semen of the same bull and extender treatment. To evaluate oxidative damage, TBARS concentration was measured to indirectly determine malondialdehyde (lipid-peroxidation metabolite) concentration, whereas TAC was measured using myoglobin, as a source of radicals, which interact with a chromogen 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS), whose cation radical is spectroscopically detectable. The latency phase (lag) in the accumulation of ABTS cation is proportional to the antioxidant concentration. The pregnancy rates 60 days after AI were analyzed by chi-square test, and data of free radical production were evaluated by ANOVA, followed by the Tukey test with significance level of 5%. Reactive oxygen species (ROS) production in nature semen (0.351 nmol of TBARS in 50 × 108 spermatozoa) was lower (P < 0.05) when compared to diluted semen (0.367 nmol of TBARS in 50 × 108 spermatozoa). No difference (P > 0.05) between extenders was observed after thawing. The bulls with poor-quality semen presented total ROS production (0.967) superior (P < 0.05) to that of bulls with good-quality semen (0.753). Although the use of antioxidant in the extender did not decrease ROS and TAC production (P > 0.05), pregnancy rates after AI in bulls with poor quality semen with AE (60.0%) was superior (P < 0.06) to that in bulls with high-quality sperm with AE (56.6%). In conclusion, bulls with poor-quality semen present higher ROS and TAC production, and the use of antioxidant in the extender for cryopreservation with poor-quality bovine semen, in spite of not decreasing ROS and TAC production, improves pregnancy rates. Financial support: FAPESP.

Detection of Ureaplasma diversum in bovine semen straws for artificial insemination

2009 ◽  
Vol 165 (19) ◽  
pp. 572-573 ◽  
Author(s):  
L. M. Marques ◽  
M. Buzinhani ◽  
R. L. Neto ◽  
R. C. Oliveira ◽  
M. Yamaguti ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Bovine Semen ◽  
Ureaplasma Diversum

scholarly journals Use of immobilized cryopreserved bovine semen in a blind artificial insemination trial

2015 ◽  
Vol 84 (3) ◽  
pp. 413-420 ◽  
Author(s):  
Fride Berg Standerholen ◽  
Karin Elisabeth Waterhouse ◽  
Anne Guro Larsgard ◽  
Randi Therese Garmo ◽  
Frøydis Deinboll Myromslien ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Bovine Semen

scholarly journals Breeding profiles at the periparturient stage in captive red foxes (Vulpes vulpes) mating naturally or subjected to artificial insemination in Japan

2019 ◽  
Vol 63 (2) ◽  
pp. 299-302
Author(s):  
Minoru Yatu ◽  
Mitsuhiro Sato ◽  
Jin Kobayashi ◽  
Toshihiro Ichijyo ◽  
Hiroshi Satoh ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Red Fox ◽  
Clinical Importance ◽  
Red Foxes ◽  
Bovine Semen ◽  
Significant Difference ◽  
Conception Rates ◽  
In Captivity ◽  
Natural Mating ◽  
Semen Extender

AbstractIntroduction: Breeding profiles at the periparturient stage in red foxes which mated naturally or were subjected to artificial insemination were retrospectively surveyed using 130 vixens during their reproductive seasons of 2012–2017 in Japan. Material and Methods: Natural mating vixens were encouraged a maximum of three times with the same male, while artificial insemination was conducted using frozen-thawed semen with the bovine semen extender as a diluent. Results: With natural mating, conception rates after one, two, and three copulations were 55.8%, 68.0%, and 85.7%, respectively, showing a significant difference between the rates for one and three copulations. Conception rates with artificial insemination were 82.4%. Mean gestation periods were between 52.1 and 53.3 days in all groups. Mean litter sizes were 3.7–4.3 cubs with natural mating, and 4.4 cubs with artificial insemination. Although some sporadic and inconsistent changes in litter sizes were noted between primiparous and multiparous groups, these were of doubtful clinical importance. Conclusion: This is the first report from Japan concerning basic breeding events of red fox vixens in captivity.

Reproductive performance of immobilized cryopreserved bovine semen used for timed artificial insemination

2017 ◽  
Vol 52 (6) ◽  
pp. 1019-1024 ◽  
Author(s):  
AH Alm-Kristiansen ◽  
G Dalen ◽  
G Klinkenberg ◽  
L Bekk ◽  
LT Thorkildsen ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Artificial Insemination ◽  
Bovine Semen ◽  
Timed Artificial Insemination

The comparative fertility of bovine semen of four ages diluted with skim-milk and skim-milk + glycerol

1959 ◽  
Vol 53 (3) ◽  
pp. 354-357 ◽  
Author(s):  
L. K. O'Connor ◽  
G. F. Smith
Keyword(s):  
Artificial Insemination ◽  
Skim Milk ◽  
Bovine Semen ◽  
Heat Treated ◽  
Split Sample ◽  
Sample Experiment

A split-sample experiment involving 14,319 first inseminations was carried out at sixteen artificial insemination centres to compare the fertility of semen diluted with skim-milk and skim-milk containing 10% glycerol when used on the first, second, third and fourth days after the day of collections.The addition of glycerol to the heat-treated skimmilk diluent significantly reduced the decline in fertility with increasing age. However, there were indications that glycerolization also reduced initial fertility.

Bovine Semen Quality Control in Artificial Insemination Centers

2021 ◽  
pp. 1019-1031
Author(s):  
Patrick Vincent ◽  
Shelley L. Underwood ◽  
Catherine Dolbec ◽  
Nadine Bouchard ◽  
Tom Kroetsch ◽  
...  
Keyword(s):  
Quality Control ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Bovine Semen
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