Progesterone, pregnanediol-3-glucuronide, relaxin and oestrone sulphate concentrations in saliva, milk and urine of female alpacas (Vicugna pacos) and their application in pregnancy diagnosis

2012 ◽  
Vol 171 (8) ◽  
pp. 195-195 ◽  
Author(s):  
J. Volkery ◽  
J. Gottschalk ◽  
A. Sobiraj ◽  
T. Wittek ◽  
A. Einspanier
Keyword(s):  
Pregnancy Diagnosis ◽  
Oestrone Sulphate ◽  
Vicugna Pacos ◽  
In Pregnancy

A rapid direct radioimmunoassay for the measurement of oestrone sulphate in the milk of dairy cows and its use in pregnancy diagnosis

1982 ◽  
Vol 95 (1) ◽  
pp. 7-12 ◽  
Author(s):  
R. J. Holdsworth ◽  
R. B. Heap ◽  
J. M. Booth ◽  
M. Hamon
Keyword(s):  
Dairy Cows ◽  
Dairy Herd ◽  
Pregnancy Diagnosis ◽  
Milk Samples ◽  
Oestrone Sulphate ◽  
Direct Assay ◽  
In Pregnancy

A radioimmunoassay for oestrone sulphate in unextracted samples of milk has been developed. The assay was validated by comparison with a method involving hydrolysis and extraction. The direct assay was used to measure oestrone sulphate in milk samples taken at weekly intervals throughout pregnancy in a commercial dairy herd. Concentrations of oestrone sulphate increased approximately 100 days after insemination and were maintained throughout the remainder of pregnancy in the range of 1·85–3·70 nmol/l.

scholarly journals COMPARISON OF A RAPID TEST WITH bPAG ELISA IN PREGNANCY DIAGNOSIS IN COWS

2021 ◽  
Vol 52 (6) ◽  
pp. 1475-1481
Author(s):  
O. I. Dana ◽  
R. H. Mukhtar ◽  
M. O. Mohammed ◽  
H. O. Dyary
Keyword(s):  
Rapid Test ◽  
Elisa Test ◽  
Urine Samples ◽  
Reference Standard ◽  
Suitable Alternative ◽  
Pregnancy Diagnosis ◽  
Alternative Method ◽  
Blood And Urine Samples ◽  
Calving Intervals ◽  
In Pregnancy

Early pregnancy detection is vital for properly managing livestock farms by re-inseminating nonpregnant females and minimizing the calving intervals. The present investigation was executed to compare a rapid test (Dairy Cow Pregnancy Test from Span Biotech Ltd. Shenzhen, China) with a commercial bPAG ELISA test (IDEXX®) for pregnancy diagnosis in non-descriptive cows. The study also aimed to elucidate if the rapid test could be an alternative method to ELISA in the field. Blood and urine samples were collected from 43 cows to measure the concentrations of bovine pregnancy-associated glycoproteins (bPAGs) in the blood samples and detect progesterone via the rapid test in the urine samples. Examining the genital tracts was achieved after slaughtering the cows to determine the uterus’ state, used as the reference standard for both tests. The results showed that the bPAG ELISA test was more accurate in pregnancy detection than the rapid test. However, there were no statistically significant differences (P > 0.05) between both methods’ results. We conclude that the rapid test can be a suitable alternative method to the bPAG ELISA test for diagnosing pregnancy in cows in field conditions.

Pregnancy diagnosis in sows based on serum oestrone sulphate concentration

1983 ◽  
Vol 113 (11) ◽  
pp. 229-233 ◽  
Author(s):  
N. Cunningham ◽  
J. Hattersley ◽  
A. Wrathall
Keyword(s):  
Pregnancy Diagnosis ◽  
Sulphate Concentration ◽  
Oestrone Sulphate

Oestrone sulphate for pregnancy diagnosis in sows

1987 ◽  
Vol 120 (1) ◽  
pp. 23-24 ◽  
Author(s):  
H. Sweeney ◽  
H. Sutherland
Keyword(s):  
Pregnancy Diagnosis ◽  
Oestrone Sulphate
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