scholarly journals Effect of histamine on proliferation of normal human adult lung fibroblasts.

Thorax ◽  
1988 ◽  
Vol 43 (7) ◽  
pp. 552-558 ◽  
Author(s):  
M Jordana ◽  
A D Befus ◽  
M T Newhouse ◽  
J Bienenstock ◽  
J Gauldie
Keyword(s):  
Lung Fibroblasts ◽  
Adult Lung ◽  
Human Adult ◽  
Normal Human ◽  
Human Adult Lung

scholarly journals Requirement for active glycogen synthase kinase-3β in TGF-β1 upregulation of connective tissue growth factor (CCN2/CTGF) levels in human gingival fibroblasts

2013 ◽  
Vol 305 (6) ◽  
pp. C581-C590 ◽  
Author(s):  
Maha Bahammam ◽  
Samuel A. Black ◽  
Siddika Selva Sume ◽  
Mohammad A. Assaggaf ◽  
Michael Faibish ◽  
...  
Keyword(s):  
Growth Factor ◽  
Glycogen Synthase ◽  
Pi3k Pathway ◽  
Tissue Growth ◽  
Lung Fibroblasts ◽  
Gingival Fibroblasts ◽  
Drug Induced ◽  
Adult Lung ◽  
Human Adult ◽  
Gsk 3Β

Connective tissue growth factor (CCN2/CTGF) mediates transforming growth factor-β (TGF-β)-induced fibrosis. Drug-induced gingival overgrowth is tissue specific. Here the role of the phosphoinositol 3-kinase (PI3K) pathway in mediating TGF-β1-stimulated CCN2/CTGF expression in primary human adult gingival fibroblasts and human adult lung fibroblasts was compared. Data indicate that PI3K inhibitors attenuate upregulation of TGF-β1-induced CCN2/CTGF expression in human gingival fibroblasts independent of reducing JNK MAP kinase activation. Pharmacologic inhibitors and small interfering (si)RNA-mediated knockdown studies indicate that calcium-dependent isoforms and an atypical isoform of protein kinase C (PKC-δ) do not mediate TGF-β1-stimulated CCN2/CTGF expression in gingival fibroblasts. As glycogen synthase kinase-3β (GSK-3β) can undergo phosphorylation by the PI3K/pathway, the effects of GSK-3β inhibitor kenpaullone and siRNA knockdown were investigated. Data in gingival fibroblasts indicate that kenpaullone attenuates TGF-β1-mediated CCN2/CTGF expression. Activation of the Wnt canonical pathways with Wnt3a, which inhibits GSK-3β, similarly inhibits TGF-β1-stimulated CCN2/CTGF expression. In contrast, inhibition of GSK-3β by Wnt3a does not inhibit, but modestly stimulates, CCN2/CTGF levels in primary human adult lung fibroblasts and is β-catenin dependent, consistent with previous studies performed in other cell models. These data identify a novel pathway in gingival fibroblasts in which inhibition of GSK-3β attenuates CCN2/CTGF expression. In adult lung fibroblasts inhibition of GSK-3β modestly stimulates TGF-β1-regulated CCN2/CTGF expression. These studies have potential clinical relevance to the tissue specificity of drug-induced gingival overgrowth.

Human SP-A1 and SP-A2 genes are differentially regulated during development and by cAMP and glucocorticoids

1994 ◽  
Vol 266 (4) ◽  
pp. L367-L374 ◽  
Author(s):  
S. M. McCormick ◽  
C. R. Mendelson
Keyword(s):  
Lung Tissue ◽  
Protein A ◽  
Fetal Lung ◽  
Companion Paper ◽  
Primer Extension ◽  
Northern Analysis ◽  
Adult Lung ◽  
Human Adult ◽  
Mrna Transcripts ◽  
Human Adult Lung

Expression of the surfactant protein A (SP-A) gene is lung specific, developmentally induced, and regulated by adenosine 3',5'-cyclic monophosphate (cAMP) and glucocorticoids. Humans have two highly similar genes encoding SP-A (SP-A1 and SP-A2). In the companion paper [S.M. McCormick, V. Boggaram, and C.R. Mendelson Am. J. Physiol. 266 (Lung Cell. Mol. Physiol. 10): L354-L366, 1994] we report that SP-A1 and SP-A2 RNA transcripts are alternatively spliced at their 5' ends, resulting in nine different primer-extended transcripts. In the present study, primer extension was used to assess the relative levels of expression of the SP-A1 and SP-A2 genes in human adult lung tissue and in fetal lung tissues maintained in organ culture in the absence or presence of dibutyryl (DB)cAMP (1 mM) and dexamethasone (Dex, 10(-4) M). Primer extension and Northern analysis were used to assess the effects of these agents on the levels of expression of these genes. In human adult lung tissue, 65% of the SP-A mRNA transcripts were derived from the SP-A2 gene, whereas only 35% were from SP-A1. On the other hand, in lung tissue from a 28-wk gestation neonate, only SP-A1 mRNA transcripts were detected, and, in midgestation fetal lung cultured in control medium, 65% of the SP-A mRNA was found to be SP-A1 and 35% was SP-A2.(ABSTRACT TRUNCATED AT 250 WORDS)

Heterogeneous Proliferative Characteristics of Human Adult Lung Fibroblast Lines and Clonally Derived Fibroblasts from Control and Fibrotic Tissue

1988 ◽  
Vol 137 (3) ◽  
pp. 579-584 ◽  
Author(s):  
Manel Jordana ◽  
Jerry Schulman ◽  
Charlie McSharry ◽  
Louis B. Irving ◽  
Michael T. Newhouse ◽  
...  
Keyword(s):  
Lung Fibroblast ◽  
Adult Lung ◽  
Human Adult ◽  
Fibrotic Tissue ◽  
Human Adult Lung

scholarly journals Numerical Modelling and Analysis of Peripheral Airway Asymmetry and Ventilation in the Human Adult Lung

2012 ◽  
Vol 134 (6) ◽  
Author(s):  
F. S. Henry ◽  
C. J. Llapur ◽  
A. Tsuda ◽  
R. S. Tepper
Keyword(s):  
Phase Iii ◽  
Adult Lung ◽  
Human Adult ◽  
Airway Trees ◽  
Structural Abnormalities ◽  
Peripheral Airway ◽  
Experimental Trend ◽  
Human Adult Lung ◽  
Conducting Airways ◽  
Phase Iii Slope

We present a new one-dimensional model of gas transport in the human adult lung. The model comprises asymmetrically branching airways, and heterogeneous interregional ventilation. Our model differs from previous models in that we consider the asymmetry in both the conducting and the acinar airways in detail. Another novelty of our model is that we use simple analytical relationships to produce physiologically realistic models of the conducting and acinar airway trees. With this new model, we investigate the effects of airway asymmetry and heterogeneous interregional ventilation on the phase III slope in multibreath washouts. The model predicts the experimental trend of the increase in the phase III slope with breath number in multibreath washout studies for nitrogen, SF6 and helium. We confirm that asymmetrical branching in the acinus controls the magnitude of the first-breath phase III slope and find that heterogeneous interregional ventilation controls the way in which the slope changes with subsequent breaths. Asymmetry in the conducting airways appears to have little effect on the phase III slope. That the increase in slope appears to be largely controlled by interregional ventilation inhomogeneities should be of interest to those wishing to use multibreath washouts to detect the location of the structural abnormalities within the lung.

scholarly journals Evidence for tissue-resident mesenchymal stem cells in human adult lung from studies of transplanted allografts

2007 ◽  
Vol 117 (4) ◽  
pp. 989-996 ◽  
Author(s):  
Vibha N. Lama ◽  
Lisa Smith ◽  
Linda Badri ◽  
Andrew Flint ◽  
Adin-Cristian Andrei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adult Lung ◽  
Human Adult ◽  
Human Adult Lung
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