scholarly journals Quantitation of Chlamydia trachomatis by culture, direct immunofluorescence and competitive polymerase chain reaction.

1995 ◽  
Vol 71 (4) ◽  
pp. 239-243 ◽  
Author(s):  
E H Frost ◽  
S Deslandes ◽  
D Bourgaux-Ramoisy ◽  
P Bourgaux
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Direct Immunofluorescence ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals CLINICAL AND IMMUNOLOGICAL CHANGES OF NON-GONOCOCCAL URETHRITIS

2020 ◽  
Vol 22 (1) ◽  
pp. 40-44
Author(s):  
Gadoev Maruf ◽  
◽  
Bakhromuddin Saidzoda ◽  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Trichomonas Vaginalis ◽  
Ureaplasma Urealyticum ◽  
Control Group ◽  
Direct Immunofluorescence ◽  
Chain Reaction ◽  
Immunological Disorders ◽  
Gonococcal Urethritis ◽  
Polymerase Chain

Objective: To study the clinical features and the state of general immunological reactivity in non-gonococcal urethritis (NGU) in men. Methods: Examined 100 men between the ages of 20 and 48 years: 75 patients of NGU (main group) and 25 healthy (control group). The average age of the patients was 26.7±1.7 years, and the male of control group was 27.9±1.7 years. Clinical, microscopic, immunological research methods were used, including direct immunofluorescence (DIF), polymerase chain reaction (PCR). Results: Ureaplasma urealyticum was found in 37 (49.3%) patients, 33 (44%) had Chlamydia trachomatis, 23 (30.7%) had Mycoplasma genitalium, 16 (21.3%) had Trichomonas vaginalis. In 24 (32%) of NGU patients had a mixed infection: in 14 (18.7%) had a combination of two STIs and in 10 (13, 3%) had three infections. In 51 (68%) of patients the process passed in the form of monoinfection. Various complaints (dysuric disorders, pain, discomfort and agglutination of the labium urethra) were presented by 51 (68%) of sick patients. The excretions from the urethra were marked in 46 (61.3%) of patients, reproductive disorders are 3 times less common. Immunological disorders were manifested by a decrease in CD4 and CD8 lymphocytes, PHA, PN and IL-10, increase – IgM, IgG, CIC, TNFα, IL-1β. Conclusions: The most common cause of NGU is Ureaplasma urealyticum and Chlamydia trachomatis. In most cases NGU proceeds in the form of monoinfection. Subjective and objective symptoms occur in 64% and 59% of patients, respectively. Immunological disorders were detected in 71% of patients. Keywords: Non-gonogococcal urethritis, direct immunofluorescence, immunoenzyme method, polymerase chain reaction

scholarly journals The use of direct immunofluorescence and nested polymerase chain reaction in diagnosing perinatal infections of Chlamydia trachomatis

2018 ◽  
Vol 27 (12) ◽  
pp. 1711-1716
Author(s):  
Magdalena Frej-Mądrzak ◽  
Dorota Teryks-Wołyniec ◽  
Piotr Jankowski ◽  
Paulina Krochmal ◽  
Jolanta Sarowska ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Direct Immunofluorescence ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain
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