scholarly journals Sensitive detection and typing of Chlamydia trachomatis using nested polymerase chain reaction.

1993 ◽  
Vol 69 (4) ◽  
pp. 290-294
Author(s):  
E H Frost ◽  
S Deslandes ◽  
D Bourgaux-Ramoisy
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Sensitive detection and differentiation ofSapporo virus, a member of the familyCaliciviridae, by standard and booster nested polymerase chain reaction

2001 ◽  
Vol 65 (2) ◽  
pp. 413-417 ◽  
Author(s):  
Shinjiro Honma ◽  
Shuji Nakata ◽  
Yoshiyuki Sakai ◽  
Masatoshi Tatsumi ◽  
Kazuko Numata-Kinoshita ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals The use of direct immunofluorescence and nested polymerase chain reaction in diagnosing perinatal infections of Chlamydia trachomatis

2018 ◽  
Vol 27 (12) ◽  
pp. 1711-1716
Author(s):  
Magdalena Frej-Mądrzak ◽  
Dorota Teryks-Wołyniec ◽  
Piotr Jankowski ◽  
Paulina Krochmal ◽  
Jolanta Sarowska ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Direct Immunofluorescence ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Using a Polymerase Chain Reaction as a Complementary Test to Improve the Detection of Dermatophyte Fungus in Nails

2014 ◽  
Vol 104 (3) ◽  
pp. 233-237 ◽  
Author(s):  
María José Iglesias Sánchez ◽  
Ana María Pérez Pico ◽  
Félix Marcos Tejedor ◽  
María Jesús Iglesias Sánchez ◽  
Raquel Mayordomo Acevedo
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Classical Method ◽  
Clinical Manifestations ◽  
Culture Method ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Traditional Procedure ◽  
Polymerase Chain

Background Dermatomycoses are a group of pathologic abnormalities frequently seen in clinical practice, and their prevalence has increased in recent decades. Diagnostic confirmation of mycotic infection in nails is essential because there are several pathologic conditions with similar clinical manifestations. The classical method for confirming the presence of fungus in nail is microbiological culture and the identification of morphological structures by microscopy. Methods We devised a nested polymerase chain reaction (PCR) that amplifies specific DNA sequences of dermatophyte fungus that is notably faster than the 3 to 4 weeks that the traditional procedure takes. We compared this new technique and the conventional plate culture method in 225 nail samples. The results were subjected to statistical analysis. Results We found concordance in 78.2% of the samples analyzed by the two methods and increased sensitivity when simultaneously using the two methods to analyze clinical samples. Now we can confirm the presence of dermatophyte fungus in most of the positive samples in just 24 hours, and we have to wait for the result of culture only in negative PCR cases. Conclusions Although this PCR cannot, at present, substitute for the traditional culture method in the detection of dermatophyte infection of the nails, it can be used as a complementary technique because its main advantage lies in the significant reduction of time used for diagnosis, in addition to higher sensitivity.

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