scholarly journals Antibiotic resistance, plasmid profile, auxotypes and serovars of Neisseria gonorrhoeae strains isolated in Sevilla (Spain).

1990 ◽  
Vol 66 (2) ◽  
pp. 87-90
Author(s):  
J C Palomares ◽  
M C Lozano ◽  
E J Perea
Keyword(s):  
Antibiotic Resistance ◽  
Neisseria Gonorrhoeae ◽  
Plasmid Profile ◽  
Resistance Plasmid

Antibiotic resistance, plasmid content and auxotypes of Neisseria gonorrhoeae in Greece

1988 ◽  
Vol 21 (5) ◽  
pp. 571-579 ◽  
Author(s):  
L. Mavrommati ◽  
E. Tzetepi ◽  
A. Sima ◽  
G. Tzanakaki ◽  
A. Kokia ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Neisseria Gonorrhoeae ◽  
Plasmid Content ◽  
Resistance Plasmid

scholarly journals Expanding U.S. Laboratory Capacity for Neisseria gonorrhoeae Antimicrobial Susceptibility Testing and Whole-Genome Sequencing through the CDC's Antibiotic Resistance Laboratory Network

2020 ◽  
Vol 58 (4) ◽  
Author(s):  
Ellen N. Kersh ◽  
Cau D. Pham ◽  
John R. Papp ◽  
Robert Myers ◽  
Richard Steece ◽  
...  
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Neisseria Gonorrhoeae ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Whole Genome ◽  
Content Type ◽  
Laboratory Capacity

ABSTRACT U.S. gonorrhea rates are rising, and antibiotic-resistant Neisseria gonorrhoeae (AR-Ng) is an urgent public health threat. Since implementation of nucleic acid amplification tests for N. gonorrhoeae identification, the capacity for culturing N. gonorrhoeae in the United States has declined, along with the ability to perform culture-based antimicrobial susceptibility testing (AST). Yet AST is critical for detecting and monitoring AR-Ng. In 2016, the CDC established the Antibiotic Resistance Laboratory Network (AR Lab Network) to shore up the national capacity for detecting several resistance threats including N. gonorrhoeae. AR-Ng testing, a subactivity of the CDC’s AR Lab Network, is performed in a tiered network of approximately 35 local laboratories, four regional laboratories (state public health laboratories in Maryland, Tennessee, Texas, and Washington), and the CDC’s national reference laboratory. Local laboratories receive specimens from approximately 60 clinics associated with the Gonococcal Isolate Surveillance Project (GISP), enhanced GISP (eGISP), and the program Strengthening the U.S. Response to Resistant Gonorrhea (SURRG). They isolate and ship up to 20,000 isolates to regional laboratories for culture-based agar dilution AST with seven antibiotics and for whole-genome sequencing of up to 5,000 isolates. The CDC further examines concerning isolates and monitors genetic AR markers. During 2017 and 2018, the network tested 8,214 and 8,628 N. gonorrhoeae isolates, respectively, and the CDC received 531 and 646 concerning isolates and 605 and 3,159 sequences, respectively. In summary, the AR Lab Network supported the laboratory capacity for N. gonorrhoeae AST and associated genetic marker detection, expanding preexisting notification and analysis systems for resistance detection. Continued, robust AST and genomic capacity can help inform national public health monitoring and intervention.

scholarly journals Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia

1999 ◽  
Vol 123 (2) ◽  
pp. 225-232 ◽  
Author(s):  
S. RADU ◽  
Y. K. HO ◽  
S. LIHAN ◽  
YUHERMAN ◽  
G. RUSUL ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Surface Water ◽  
Vibrio Cholerae ◽  
Genetic Relatedness ◽  
Profile Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Plasmid Profile ◽  
Significant Public Health ◽  
Resistance Patterns ◽  
Wide Variability

A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to antibiotics, plasmid profiles and random amplified polymorphic DNA (RAPD) analysis. All were resistant to 9 or more of the 17 antibiotics tested. Identical antibiotic resistance patterns of the isolates may indicate that they share a common mode of developing antibiotic resistance. Furthermore, the multiple antibiotic resistance indexing showed that all strains tested originated from high risk contamination. Plasmid profile analysis by agarose gel electrophoresis showed the presence of small plasmids in 12 (7 non-O1 and 5 O1 serotypes) with sizes ranging 1·3–4·6 MDa. The CT gene was detected in all clinical isolates but was present in only 14 (6 O1 serotype and 8 non-O1 serotype) isolates from environmental waters. The genetic relatedness of the clinical and environmental Vibrio cholerae O1 and non-O1 strains was investigated by RAPD fingerprinting with four primers. The four primers generated polymorphisms in all 31 strains of Vibrio cholerae tested, producing bands ranging from <250 to 4500 bp. The RAPD profiles revealed a wide variability and no correlation with the source of isolation. This study provides evidence that Vibrio cholerae O1 and non-O1 have significant public health implications.

Large antibiotic-resistance plasmid of Edwardsiella tarda contributes to virulence in fish

2012 ◽  
Vol 52 (5) ◽  
pp. 259-266 ◽  
Author(s):  
Jong Earn Yu ◽  
Mi Young Cho ◽  
Jin-woo Kim ◽  
Ho Young Kang
Keyword(s):  
Antibiotic Resistance ◽  
Edwardsiella Tarda ◽  
Resistance Plasmid

scholarly journals Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Daouda A. K. Traore ◽  
Jessica A. Wisniewski ◽  
Sarena F. Flanigan ◽  
Paul J. Conroy ◽  
Santosh Panjikar ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Antibiotic Resistance ◽  
Resistance Plasmid

Transcription of the Transfer Genes traY and traM of the Antibiotic Resistance Plasmid R100-1 Is Linked

Plasmid ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 35-48 ◽  
Author(s):  
David Stockwell ◽  
Vera Lelianova ◽  
Teresa Thompson ◽  
Walter B Dempsey
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Plasmid ◽  
Transfer Genes
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