scholarly journals The effect of oral cimetidine on the basal and stimulated values of prolactin, thyroid stimulating hormone, follicle stimulating hormone and luteinizing hormone.

1980 ◽  
Vol 56 (651) ◽  
pp. 26-29 ◽  
Author(s):  
G. F. Nelis ◽  
J. G. Van de Meene
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Oral Cimetidine ◽  
Stimulating Hormone

PRODUCTION OF ANTISERA AGAINST HIGHLY PURIFIED HUMAN FOLLICLE-STIMULATING HORMONE, LUTEINIZING HORMONE AND THYROID-STIMULATING HORMONE

1976 ◽  
Vol 70 (3) ◽  
pp. 335-344 ◽  
Author(s):  
J. I. THORELL ◽  
B. HOLMSTRÖM
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
High Titre ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Antigenic Determinants ◽  
High Avidity ◽  
Cross Reactions ◽  
Stimulating Hormone

SUMMARY Antisera were produced in rabbits against highly purified preparations of human LH (2000 or 10000 i.u./mg), human FSH (5500 i.u./mg), and human TSH (7·5 i.u./mg). Most rabbits produced antisera of high titre and high avidity. Cross-reactions were minimal between human TSH and human chorionic gonadotrophin (HCG) and between human FSH and HCG but marked between human LH and HCG. TSH and FSH also showed a constant but relatively weak cross-reaction. LH cross-reacted with FSH to a higher degree than did HCG. The avidity of the antisera was high. It was concluded that much of the lack of specificity recorded for glycoprotein antisera are effects of impure immunogens. Some of the true cross-reactions are probably explained by shared antigenic determinants of the β-subunits. Unadsorbed antisera could be used for assay of FSH and TSH in plasma from pregnant women.

DEXTRAN-COATED CHARCOAL USED IN THE RADIOIMMUNOASSAY OF HUMAN PITUITARY LUTEINIZING HORMONE

1973 ◽  
Vol 73 (3) ◽  
pp. 444-454 ◽  
Author(s):  
T. Sand ◽  
P. A. Torjesen
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Protein Composition ◽  
Thyroid Stimulating Hormone ◽  
Human Chorionic Gonadotrophin ◽  
Human Thyroid ◽  
Serum Samples ◽  
Human Pituitary ◽  
The Difference ◽  
Stimulating Hormone

ABSTRACT A radioimmunoassay for human pituitary luteinizing hormone (LH) using charcoal for the separation of free from antibody-bound hormone is described. The ability of the various types of charcoal preparations tested to separate free from antibody-bound hormone differed greatly as did the amount required to give maximum adsorption of free hormone. It was also found that the adsorption of free and antibody-bound hormone was greatly influenced by the presence of other proteins. Hence it was necessary to add human serum to the standard tubes before the addition of the charcoal-dextran suspension, in order to compensate for the difference in protein composition between the standards and the serum samples. Two antisera obtained from rabbits immunized with human chorionic gonadotrophin (HCG) were used. One of the antisera had an affinity to human thyroid-stimulating hormone (TSH) and human follicle-stimulating hormone (FSH) of less than 10% as compared to that of LH, while the other had an affinity of about 30 % as compared to that of LH.

A SOLID-PHASE RADIOIMMUNOASSAY FOR OVINE FOLLICLE-STIMULATING HORMONE

1973 ◽  
Vol 58 (2) ◽  
pp. 239-249 ◽  
Author(s):  
N. F. CUNNINGHAM ◽  
C. NANCY HEBERT
Keyword(s):  
Luteinizing Hormone ◽  
Blood Plasma ◽  
Solid Phase ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Cross Reaction ◽  
Sheep Blood ◽  
Solid Phase Radioimmunoassay ◽  
Stimulating Hormone

SUMMARY A radioimmunoassay using antibody-coated polystyrene tubes is described, which is suitable for the estimation of follicle-stimulating hormone (FSH) levels in sheep blood plasma. Ovine FSH was labelled with 125I by an enzymic method employing lactoperoxidase and H2O2, and was stable for several months at −15 °C. Antisera to ovine FSH were raised in rabbits. Standard curves for unlabelled FSH obtained by this method with different preparations of labelled FSH showed reproducible slopes from day to day. Ovine luteinizing hormone (LH) and thyroid-stimulating hormone (TSH) showed about 17% cross-reaction in the assay, necessitating the application of a correction to the FSH values for samples of plasma containing more than 15 ng of LH or TSH/ml. The FSH content of plasma from wethers and anoestrous ewes ranged from 20 to 120 ng/ml.

hCG production and activity during pregnancy

2001 ◽  
Vol 12 (3) ◽  
pp. 191-208 ◽  
Author(s):  
H S Randeva ◽  
A Jackson ◽  
E Karteris ◽  
E W Hillhouse
Keyword(s):  
Luteinizing Hormone ◽  
Chorionic Gonadotropin ◽  
Essential Role ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Β Subunit ◽  
Glycoprotein Hormone ◽  
Α Subunit ◽  
Hormone Specificity ◽  
Stimulating Hormone

Human chorionic gonadotropin (hCG) has an essential role in early pregnancy. It is a member of the glycoprotein hormone family also comprising the pituitary derived follicle stimulating hormone (FSH), luteinizing hormone (LH) and thyroid stimulating hormone (TSH). Each hormone consists of a non-covalently bound α and β subunit. Within a species the α subunit is identical and hormone specificity is determined by the unique β subunit.

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