scholarly journals 5' flanking sequence of the human immediate early responsive gene ccn1 (cyr61) and mapping of polymorphic CA repeat sequence motifs in the human ccn1 (cyr61) locus

2001 ◽  
Vol 54 (3) ◽  
pp. 170-175 ◽  
Author(s):  
N Schutze
Keyword(s):  
Repeat Sequence ◽  
Immediate Early ◽  
Sequence Motifs ◽  
Flanking Sequence ◽  
Ca Repeat ◽  
Responsive Gene

scholarly journals Isolation and mapping of a polymorphic CA repeat sequence at the human VRK1 locus

1999 ◽  
Vol 44 (2) ◽  
pp. 133-134
Author(s):  
Jun Sugimoto ◽  
Toshihiro Yamauchi ◽  
Toyomasa Hatakeyama ◽  
Masaharu Isobe ◽  
J. Sugimoto ◽  
...  
Keyword(s):  
Repeat Sequence ◽  
Ca Repeat

scholarly journals Genomic organization of the glycoprotein D gene: Duffy blood group Fya/Fyb alloantigen system is associated with a polymorphism at the 44- amino acid residue

Blood ◽  
1995 ◽  
Vol 85 (3) ◽  
pp. 622-626 ◽  
Author(s):  
S Iwamoto ◽  
T Omi ◽  
E Kajii ◽  
S Ikemoto
Keyword(s):  
Amino Acid ◽  
Blood Group ◽  
Direct Repeat ◽  
Base Change ◽  
Repeat Sequence ◽  
Blood Group System ◽  
Glycoprotein D ◽  
Flanking Sequence ◽  
Duffy Blood Group ◽  
Flanking Region

The Duffy blood group antigen has been characterized by its roles on red blood cells: as a receptor for the malarial parasites and as a promiscuous receptor for chemokine superfamily. Recently, the Duffy blood group associated glycoprotein D (gpFy) cDNA has been cloned (Chaudhuri et al: Proc Natl Acad Sci USA 90:10793, 1993). In this report we describe the organization of genomic DNA coding for the gpFy and elucidate the molecular nature of Fya/b polymorphisms. By a Southern blotting analysis probed with gpFy cDNA, gpFy gene was shown to be composed of three DNA fragments; 1.1-kb Sac I, 1.9-kb EcoRI, and their intervening 47-bp fragments. We cloned the 1.1-kb Sac I and 1.9- kb EcoRI fragments by inverted polymerase chain reaction (IPCR) procedure. The promoter region of the gpFy gene was cloned by IPCR of 1.1-kb Sac I fragment and the 3′ flanking sequence was cloned by IPCR of 1.9 kb EcoRI fragment. The both IPCR products contained on both side the known gpFy cDNA sequence without introns, as expected. Although no TATA or CCAAT boxes are present in the promoter sequence, several transcription factor binding site motifs are contained, including AP-1, HNF-5, TCF-1, ApoE B2, W-element, H-APF-1, and Sp-1. The 3′ flanking region has two additional polyadenylation signals, other than that used in the cDNA, and also has an indirect and a direct repeat sequence clustered with the 5′ flanking region. These facts indicate a possibility that the gpFy gene has been evolved by multiple retrotransposition events. By comparing the coding area of the gpFy gene in 28 Duffy-positive individuals, we elucidated that one base change that results in an amino acid substitution [GA-T(Asp44)-- >GGT(Gly)] is in accordance with the Fya/Fyb polymorphism. This fact proves that the gpFy cDNA and its gene described in this report encode the Duffy blood group system.

Gentamicin inhibits rat renal cortical homotypic endosomal fusion: role of megalin

1997 ◽  
Vol 272 (1) ◽  
pp. F117-F123 ◽  
Author(s):  
T. G. Hammond ◽  
R. R. Majewski ◽  
J. H. Kaysen ◽  
F. O. Goda ◽  
G. L. Navar ◽  
...  
Keyword(s):  
Damage Mechanism ◽  
Repeat Sequence ◽  
Sequence Motifs ◽  
Polyaspartic Acid ◽  
In Vitro Reconstitution ◽  
Proximal Tubular ◽  
Renal Proximal Tubular Cells ◽  
Renal Proximal Tubular

Megalin, a giant glycoprotein receptor heavily concentrated in the early endosomal pathway of renal proximal tubular cells, binds gentamicin with high affinity and delivers the drug to lysosomes. Utilizing an in vitro reconstitution assay we tested whether gentamicin-induced vacuolation is associated with inhibition of early endosomal fusion, as well as whether megalin plays a role in mediating these effects. Pretreatment of rats with gentamicin inhibited rat renal proximal tubular homotypic endosomal fusion. Administered simultaneously, gentamicin and polymers of polyaspartic acid, which protect against the hemodynamic effects of gentamicin nephrotoxicity, had no net effect on fusion. Polyaspartic acid alone had no effect on fusion. Antisera to the tail of the megalin/gentamicin receptor inhibited fusion, whereas non-specific controls had no effect. Peptides matching homologous NPXY repeat sequence motifs in the cytosolic tail stimulated endosomal fusion, whereas reverse sequence control peptides had no effect. These data suggest that gentamicin inhibition of endosomal fusion in the renal proximal tubule is a damage mechanism mediated by specific peptide sequences in the cytosolic tail of the giant gentamicin-binding receptor megalin and that receptors can effect the fusion properties of membranes in which they reside.

scholarly journals A polymorphic CA repeat sequence at the human calcitonin locus

1998 ◽  
Vol 43 (2) ◽  
pp. 146-147 ◽  
Author(s):  
Kazuhiro Tsukamoto ◽  
M. Emi
Keyword(s):  
Repeat Sequence ◽  
Human Calcitonin ◽  
Ca Repeat

scholarly journals Assignment of the human poly(A) polymerase (PAP) gene to chromosome 14q32.1–q32.2 and isolation of a polymorphic CA repeat sequence

1999 ◽  
Vol 44 (4) ◽  
pp. 253-255 ◽  
Author(s):  
Toshihiro Yamauchi ◽  
J. Sugimoto ◽  
Toyomasa Hatakeyama ◽  
Shuichi Asakawa ◽  
Nobuyoshi Shimizu ◽  
...  
Keyword(s):  
Repeat Sequence ◽  
Ca Repeat

scholarly journals Negative and positive regulation by a short segment in the 5'-flanking region of the human cytomegalovirus major immediate-early gene.

1987 ◽  
Vol 7 (11) ◽  
pp. 4125-4129 ◽  
Author(s):  
J A Nelson ◽  
C Reynolds-Kohler ◽  
B A Smith
Keyword(s):  
Human Cytomegalovirus ◽  
Regulatory Element ◽  
Regulatory Region ◽  
Fold Increase ◽  
Immediate Early Gene ◽  
Dnase I ◽  
Early Gene ◽  
Immediate Early ◽  
Flanking Sequence ◽  
Hypersensitive Sites

To analyze the significance of inducible DNase I-hypersensitive sites occurring in the 5'-flanking sequence of the major immediate-early gene of human cytomegalovirus (HCMV), various deleted portions of the HCMV immediate-early promoter regulatory region were attached to the chloramphenicol acetyltransferase (CAT) gene and assayed for activity in transiently transfected undifferentiated and differentiated human teratocarcinoma cells, Tera-2. Assays of progressive deletions in the promoter regulatory region indicated that removal of a 395-base-pair portion of this element (nucleotides -750 to -1145) containing two inducible DNase I sites which correlate with gene expression resulted in a 7.5-fold increase in CAT activity in undifferentiated cells. However, in permissive differentiated Tera-2, human foreskin fibroblast, and HeLa cells, removal of this regulatory region resulted in decreased activity. In addition, attachment of this HCMV upstream element to a homologous or heterologous promoter increased activity three- to fivefold in permissive cells. Therefore, a cis regulatory element exists 5' to the enhancer of the major immediate-early gene of HCMV. This element negative modulates expression in nonpermissive cells but positively influences expression in permissive cells.

Sign in / Sign up

Export Citation Format

Share Document