scholarly journals Applications of polymerase chain reaction-single stranded conformational polymorphism to microbiology

1996 ◽  
Vol 49 (6) ◽  
pp. M315-M320 ◽  
Author(s):  
J R Kerr ◽  
M D Curran
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain

Variation in ovine KRTAP8-1 is associated with variation in wool fibre staple strength and curvature

2019 ◽  
Vol 157 (6) ◽  
pp. 550-554 ◽  
Author(s):  
H. Gong ◽  
H. Zhou ◽  
W. Li ◽  
J. Wang ◽  
S. Li ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genetic Marker ◽  
Protein Gene ◽  
Wool Fibre ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Conformational Polymorphism ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain ◽  
Staple Strength

AbstractKRTAP8-1 was the initial high-glycine-tyrosine keratin-associated protein gene recognized in sheep, but little is known about the functional influence of this gene. The current study used polymerase chain reaction-single stranded conformational polymorphism analysis to genotype KRTAP8-1 in 391 Southdown × Merino-cross sheep from six sire-lines. Five previously described variants (named A to E) of KRTAP8-1 were identified with frequencies of 67.0, 14.2, 7.0, 10.7 and 1.0%, respectively. Of the four variants (A, B, C and D) that occurred at a frequency greater than 5%, the presence of C was found to be associated with a reduction in mean fibre curvature (MFC) and the presence of D was associated with an increase in mean staple strength (MSS), whereas the presence of A had a trend of association with reduced MSS. Associations were not identified with other wool traits. These results suggest that variation in KRTAP8-1 affects MSS and MFC, and that KRTAP8-1 has the potential to be used as a genetic marker for improving these traits.

scholarly journals Application of Polymerase Chain Reaction–Single Strand Conformational Polymorphism (PCR–SSCP) to Identification of Flatfish Species

1999 ◽  
Vol 82 (4) ◽  
pp. 903-907 ◽  
Author(s):  
Ana Céspedes ◽  
Teresa García ◽  
Esther Carrera ◽  
Isabel González ◽  
Esther Carrera ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Single Strand ◽  
Chain Reaction ◽  
Greenland Halibut ◽  
Single Strand Conformational Polymorphism ◽  
Conformational Polymorphism ◽  
Flatfish Species ◽  
Mitochondrial Cytochrome B ◽  
Polymerase Chain

Abstract A method of DNA analysis based on polymerase chain reaction–single strand conformational polymorphism (PCR–SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by native polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).

Application of polymerase chain reaction for detection of goats' milk adulteration by milk of cow

2001 ◽  
Vol 68 (2) ◽  
pp. 333-336 ◽  
Author(s):  
JACEK BANIA ◽  
MACIEJ UGORSKI ◽  
ANTONI POLANOWSKI ◽  
ERYK ADAMCZYK
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Analysis ◽  
Marker Gene ◽  
Meat Products ◽  
Universal Primers ◽  
Animal Origin ◽  
Chain Reaction ◽  
Specific Primers ◽  
Single Stranded Conformational Polymorphism ◽  
Polymerase Chain

Numerous methods based on DNA analysis have been employed in the food industry to monitor adulterations of food products of animal origin. Among them the most frequently used are: polymerase chain reaction (PCR) amplification of a marker gene fragment(s) with universal primers, or amplification of DNA with species-specific primers. PCR-products of different origin can be discriminated by size, restriction fragment length polymorphism (RFLP) or single stranded conformational polymorphism (SSCP) analysis. These methods have been used for identification, and differentiation between, the animal origins of raw or heat-treated meat and meat products (Chikuni et al. 1994; Meyer et al. 1994, 1995; Zehner et al. 1998; Behrens et al. 1999; Guoli et al. 1999; Hopwood et al. 1999; Matsunaga et al. 1999; Wolf et al. 1999). These approaches are also applicable to the analysis of dairy products. However, adulterations of goats' milk and its products are traditionally tested by immunological and/or electrophoretic methods (Amigo et al. 1992; Levieux & Venien, 1994; Mimmo & Pagani, 1998). So far, only a few DNA-based techniques designed to detect the presence of bovine DNA in goats' milk have been described (Plath et al. 1997; Branciari et al. 2000). This paper presents a one-step PCR procedure for detection of adulteration of goats' milk with cows' milk. The method, employing bovine-specific primers for amplification of a 274 bp fragment of cytochrome b DNA, seems to be simple, fast, specific and sensitive.

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