scholarly journals The role of a nested polymerase chain reaction in the diagnosis of Pneumocystis carinii pneumonia

1995 ◽  
Vol 48 (6) ◽  
pp. M347-M350 ◽  
Author(s):  
R Evans ◽  
A W L Joss ◽  
D Parratt ◽  
T H Pennington ◽  
D O Ho-Yen
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Carinii Pneumonia ◽  
Pneumocystis Carinii ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Rapid Simple and Nested Polymerase Chain Reaction for the Diagnosis of Pneumocystis carinii pneumonia

1995 ◽  
Vol 27 (4) ◽  
pp. 358-362 ◽  
Author(s):  
FranÇOise Moonens ◽  
Corinne Liesnard ◽  
FranÇOise Brancart ◽  
Jean Paul Van Vooren ◽  
Elisabeth Serruys
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Carinii Pneumonia ◽  
Pneumocystis Carinii ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Plasma (1.RAR.3)-.BETA.-D-glucan Measurement and Polymerase Chain Reaction on Sputum as Practical Parameters in Pneumocystis carinii Pneumonia.

1998 ◽  
Vol 37 (7) ◽  
pp. 618-621 ◽  
Author(s):  
Keiji OKAMOTO ◽  
Tetsuro YAMAMOTO ◽  
Daisuke NONAKA ◽  
Hirokazu SHIN ◽  
Kaoru OKADA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Carinii Pneumonia ◽  
Pneumocystis Carinii ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of NaturalToxoplasma gondiiInfection in Chicken in Thika Region of Kenya Using Nested Polymerase Chain Reaction

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
John Mokua Mose ◽  
John Maina Kagira ◽  
Simon Muturi Karanja ◽  
Maina Ngotho ◽  
David Muchina Kamau ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Toxoplasma Gondii ◽  
Human Beings ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Free Range ◽  
Age Dependent ◽  
Polymerase Chain ◽  
The Difference

The detection ofToxoplasma gondiiin free-range chickens is a good indicator of possible risk to human beings. The aim of this study was to investigate the occurrence ofT. gondiiin free-range chicken using polymerase chain reaction (PCR). Brain samples from 105 free-range chickens from three administrative areas in Thika region, Kenya, were collected, DNA-extracted, and analyzed using PCR to detect presence ofT. gondii. The overall prevalence ofT. gondiiin all the three areas was 79.0% (95% CI: 70.0–86.4%) and the prevalence across the three areas was not significantly different (P=0.5088;χ2=1.354). Female chickens had higher (79.4%) prevalence than males (78.6%), although the difference was not significant (P=0.922,χ2= 0.01). However, chickens that were more than 2 years old had significantly (P=0.003;χ2= 11.87) higher prevalence compared to younger ones. The study indicates that there was a high occurrence ofT. gondiiinfection in free-range chickens from Thika region and that the infection rate is age dependent. Further studies should be carried out to determine the possible role of roaming chickens in the epidemiology of the disease among humans in the area.

Pneumocystis carinii in Bronchoalveolar Lavage and Induced Sputum: Detection with a Nested Polymerase Chain Reaction

1995 ◽  
Vol 27 (4) ◽  
pp. 363-367 ◽  
Author(s):  
Jens Skøt ◽  
Axel Gustav Lerche ◽  
Hans Jørn Kolmos ◽  
Jens Ole Nielsen ◽  
Lars Reinhardt Mathiesen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bronchoalveolar Lavage ◽  
Pneumocystis Carinii ◽  
Induced Sputum ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain

Direct monitoring as well as sensitive diagnosis of Pneumocystis carinii pneumonia by the polymerase chain reaction on sputum samples

1993 ◽  
Vol 7 (6) ◽  
pp. 419-424 ◽  
Author(s):  
Shinichi Oka ◽  
Kazuhiro Kitada ◽  
Takami Kohjin ◽  
Yoshikazu Nakamura ◽  
Satoshi Kimura ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Carinii Pneumonia ◽  
Pneumocystis Carinii ◽  
Chain Reaction ◽  
Polymerase Chain

Variable efficiency of three primer pairs for the diagnosis of Pneumocystis carinii pneumonia by the polymerase chain reaction

1995 ◽  
Vol 9 (5) ◽  
pp. 333-340 ◽  
Author(s):  
Andrea De Luca ◽  
Enrica Tamburrini ◽  
Elena Ortona ◽  
Paola Mencarini ◽  
Paola Margutti ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Pneumocystis Carinii Pneumonia ◽  
Pneumocystis Carinii ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Variable Efficiency
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