scholarly journals Solid phase enzyme linked immunosorbent assay for classical complement activation pathway.

1987 ◽  
Vol 40 (2) ◽  
pp. 237-239 ◽  
Author(s):  
M Rhen
Keyword(s):  
Immunosorbent Assay ◽  
Complement Activation ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Activation Pathway ◽  
Complement Activation Pathway ◽  
Classical Complement

scholarly journals Distinct Different Contributions of the Alternative and Classical Complement Activation Pathway for the Innate Host Response during Sepsis

2011 ◽  
Vol 186 (5) ◽  
pp. 3066-3075 ◽  
Author(s):  
Katja Dahlke ◽  
Christiane D. Wrann ◽  
Oliver Sommerfeld ◽  
Maik Soßdorf ◽  
Peter Recknagel ◽  
...  
Keyword(s):  
Complement Activation ◽  
Host Response ◽  
Activation Pathway ◽  
Complement Activation Pathway ◽  
Classical Complement

scholarly journals Involvement of the Lectin Pathway of Complement Activation in Antimicrobial Immune Defense during Experimental Septic Peritonitis

2004 ◽  
Vol 72 (9) ◽  
pp. 5247-5252 ◽  
Author(s):  
Michaela Windbichler ◽  
Bernd Echtenacher ◽  
Thomas Hehlgans ◽  
Jens C. Jensenius ◽  
Wilhelm Schwaeble ◽  
...  
Keyword(s):  
Complement Activation ◽  
Expression Profiles ◽  
Alternative Pathway ◽  
Immune Defense ◽  
Lectin Pathway ◽  
Alternative Pathways ◽  
Activation Pathway ◽  
Deficient Mice ◽  
Complement Activation Pathway ◽  
Classical Complement

ABSTRACT A critical first line of defense against infection is constituted by the binding of natural antibodies to microbial surfaces, activating the complement system via the classical complement activation pathway. In this function, the classical activation pathway is supported and amplified by two antibody-independent complement activation routes, i.e., the lectin pathway and the alternative pathway. We studied the contribution of the different complement activation pathways in the host defense against experimental polymicrobial peritonitis induced by cecal ligation and puncture by using mice deficient in either C1q or factors B and C2. The C1q-deficient mice lack the classical complement activation pathway. While infection-induced mortality of wild-type mice was 27%, mortality of C1q-deficient mice was increased to 60%. Mice with a deficiency of both factors B and C2 lack complement activation via the classical, the alternative, and the lectin pathways and exhibit a mortality of 92%, indicating a significant contribution of the lectin and alternative pathways of complement activation to survival. For 14 days after infection, mannan-binding lectin (MBL)-dependent activation of C4 was compromised. Serum MBL-A and MBL-C levels were significantly reduced for 1 week, possibly due to consumption. mRNA expression profiles did not lend support for either of the two MBL genes to respond as typical acute-phase genes. Our results demonstrate a long-lasting depletion of MBL-A and MBL-C from serum during microbial infection and underline the importance of both the lectin and the alternative pathways for antimicrobial immune defense.

A New Technique in Quantitative Immunohematology: Solid-Phase Kinetic Enzyme-Linked Immunosorbent Assay

Vox Sanguinis ◽  
1983 ◽  
Vol 45 (6) ◽  
pp. 440-448 ◽  
Author(s):  
S. Spitalnik ◽  
J. Cowles ◽  
M.T. Cox ◽  
D. Baker ◽  
J. Holt ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Solid Phase ◽  
New Technique ◽  
Enzyme Linked Immunosorbent Assay ◽  
A New Technique

Enzyme-linked immunosorbent assay for detection of antibodies to extractable nuclear antigens in systemic lupus erythematosus, with nylon as solid phase.

1983 ◽  
Vol 29 (5) ◽  
pp. 823-827 ◽  
Author(s):  
M Ishaq ◽  
R Ali
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Prolonged Storage ◽  
Antibody Activity ◽  
Systemic Lupus ◽  
Nuclear Antigens ◽  
As Solid Phase

Abstract In this enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against extractable nuclear antigens (ENA) in sera of patients with systemic lupus erythematosus (SLE), nylon is used as solid phase for antigen binding instead of the commonly used polystyrene surface. Optimal conditions for activation of the nylon beads, antigen coating, and other relevant factors have been investigated. We compared the incidence of anti-ENA antibodies in SLE, using chromogenic and fluorogenic enzyme substrates. Of SLE patients, 54% were positive for anti-ENA antibodies when chromogenic substrate was used as compared with 68% for fluorogenic substrate. Antibody activity against Sm and RNP antigens was distinguished on the basis of ribonuclease sensitivity of the RNP antigen. The method described offers advantages such as decreased background activity, increased surface area, facility for prolonged storage of antigen-coated solid phase, and miniaturization of the assay.

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