scholarly journals Nephelometry v differential agglutination titre in the measurement of rheumatoid factors.

1981 ◽  
Vol 34 (4) ◽  
pp. 396-399 ◽  
Author(s):  
M H Pritchard ◽  
K Jobbins
Keyword(s):  
Rheumatoid Factors ◽  
Agglutination Titre

scholarly journals Human rheumatoid factor crossidiotypes. I. WA and BLA are heat-labile conformational antigens requiring both heavy and light chains.

1986 ◽  
Vol 164 (5) ◽  
pp. 1809-1814 ◽  
Author(s):  
V Agnello ◽  
J L Barnes
Keyword(s):  
Rheumatoid Factor ◽  
Primary Structure ◽  
Binding Activity ◽  
Light Chains ◽  
Heat Denaturation ◽  
Antigen Binding ◽  
Rheumatoid Factors ◽  
Simple Method ◽  
Heat Labile

Evidence was obtained that both the WA and BLA crossidiotype (XId) groups are conformational antigens requiring both L and H chains and that with heat denaturation the antigens that define the XIds and antigen-binding activity are lost in parallel. In contrast, the primary structure-dependent crossreactive idiotype (CRI), PSL2, which is only weakly detected on native Wa and Bla monoclonal rheumatoid factors (mRFs), became prominently detected on the heated Wa and Bla mRFs. Heat denaturation may provide a simple method for distinguishing Ids determined by conformational antigen from primary structure-dependent Ids. In addition to heat denaturation, some acid conditions commonly used for preparation of RFs were also found to cause marked loss of Id antigen. The finding of PSL2-CRI on Bla mRF indicates that this Id is not unique to the WA XId.

Epidemiological and Genetic Aspects of IgM Rheumatoid Factors

1988 ◽  
Vol 17 (sup75) ◽  
pp. 213-218 ◽  
Author(s):  
G. Husby ◽  
J. T. Gran ◽  
A. Johannessen
Keyword(s):  
Rheumatoid Factors

IgM-Rheumatoid Factors Measured by ELISA in Non-rheumatoid Sera: Comparison of Human and Rabbit Fc Fragments as Antigens

Rheumatology ◽  
1992 ◽  
Vol 31 (2) ◽  
pp. 141-141
Author(s):  
K. AILUS ◽  
L. MELAMIES ◽  
T. TUOMI ◽  
T. PALOSUO ◽  
K. AHO
Keyword(s):  
Rheumatoid Factors

Analysis of Monoclonal Rheumatoid Factors obtained from the B-Cell Repertoire in Rheumatoid Arthritis

1992 ◽  
Vol 35 (2) ◽  
pp. 149-157 ◽  
Author(s):  
M. ABDERRAZIK ◽  
M. MOYNIER ◽  
R JEFFFRIS ◽  
R. A. K. MAGEED ◽  
B. COMBE ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
B Cell ◽  
Rheumatoid Factors ◽  
Cell Repertoire ◽  
B Cell Repertoire

scholarly journals Genetic analysis of self-associating immunoglobulin G rheumatoid factors from two rheumatoid synovia implicates an antigen-driven response.

1992 ◽  
Vol 175 (3) ◽  
pp. 831-842 ◽  
Author(s):  
T Olee ◽  
E W Lu ◽  
D F Huang ◽  
R W Soto-Gil ◽  
M Deftos ◽  
...  
Keyword(s):  
Amino Acid ◽  
Heavy Chain ◽  
Immunoglobulin M ◽  
Natural Antibody ◽  
Antibody Repertoire ◽  
Rheumatoid Factors ◽  
Variable Regions ◽  
Genetic Origins ◽  
V Genes ◽  
Vh Gene

Although much has been learned about the molecular basis of immunoglobulin M (IgM) rheumatoid factors (RFs) in healthy individuals and in patients with mixed cryoglobulinemia and rheumatoid arthritis, little is known about the genetic origins of the potentially pathogenic IgG RFs in the inflamed rheumatoid synovia of patients. Recently, we generated from unmanipulated synovium B cells several hybridomas that secreted self-associating IgG RFs. To delineate the genetic origins of such potentially pathogenic RFs, we adapted the anchored polymerase chain reaction to rapidly clone and characterize the expressed Ig V genes for the L1 and the D1 IgG RFs. Then, we identified the germline counterparts of the expressed L1 IgG RF V genes. The results showed that the L1 heavy chain was encoded by a Vh gene that is expressed preferentially during early ontogenic development, and that is probably located within 240 kb upstream of the Jh locus. The overlap between this RF Vh gene and the restricted fetal antibody repertoire is reminiscent of the natural antibody-associated Vh genes, and suggests that at least part of the "potential pathogenic" IgG RFs in rheumatoid synovium may derive from the "physiological" natural antibody repertoire in a normal immune system. Indeed, the corresponding germline Vh gene for L1 encodes the heavy chain of an IgM RF found in a 19-wk-old fetal spleen. Furthermore, the comparisons of the expressed RF V genes and their germline counterparts reveal that the L1 heavy and light chain variable regions had, respectively, 16 and 7 somatic mutations, which resulted in eight and four amino acid changes. Strikingly, all eight mutations in the complementarity determining regions of the V gene-encoded regions were replacement changes, while only 6 of 11 mutations in the framework regions caused amino acid changes. Combined with L1's high binding affinity toward the Fc fragment, these results suggest strongly that the L1 IgG RF must have been driven by the Fc antigen.

Rheumatoid Factors and Rheumatoid Arthritis

1988 ◽  
Vol 17 (sup74) ◽  
pp. 41-44 ◽  
Author(s):  
Kimmo Aho ◽  
Tiinamaija Tuomi ◽  
Markku Heliövaara ◽  
Timo Palosuo
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factors

Polyclonal human rheumatoid factors cross-reacting with histone H3: Characterization of an idiotope on the H3 binding site

1990 ◽  
Vol 10 (4) ◽  
pp. 211-219 ◽  
Author(s):  
Thierry Martin ◽  
Anne Marie Knapp ◽  
Sylvianne Muller ◽  
Jean Louis Pasquali
Keyword(s):  
Binding Site ◽  
Histone H3 ◽  
Rheumatoid Factors

Development of Rheumatoid Factors and Anti-F(ab′)2 Antibodies in Guinea Pigs Immunized with Type II Bovine Collagen

1986 ◽  
Vol 80 (2) ◽  
pp. 214-220 ◽  
Author(s):  
Benjamin Wolf ◽  
Reza I. Bashey ◽  
Charles D. Newton ◽  
Sergio A. Jimenez
Keyword(s):  
Guinea Pigs ◽  
Type Ii ◽  
Rheumatoid Factors ◽  
Bovine Collagen
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