scholarly journals Comparison of the indirect fluorescent antibody test with agglutination, complement-fixation, and Coombs tests for Brucella antibody

1970 ◽  
Vol 23 (2) ◽  
pp. 161-165 ◽  
Author(s):  
J. M. B. Edwards ◽  
A. J. Tannahill ◽  
C. M. P. Bradstreet
Keyword(s):  
Complement Fixation ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Indirect Fluorescent Antibody

INDIRECT FLUORESCENT ANTIBODY METHOD IN SERODIAGNOSIS OF TOXOPLASMOSIS

1962 ◽  
Vol 8 (4) ◽  
pp. 545-554 ◽  
Author(s):  
A. E. Kelen ◽  
L. Ayllon-Leindl ◽  
N. A. Labzoffsky
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Fixation Test ◽  
The Other ◽  
Staining Procedure ◽  
Indirect Fluorescent Antibody ◽  
Serological Tests

The principle of the indirect fluorescent antibody-staining procedure was adapted for use in the serodiagnosis of toxoplasmosis and the technique compared with other serological tests most commonly employed at present.The results obtained with normal and immune rabbit sera as well as human patients' sera indicate that the indirect fluorescent antibody test for toxoplasmosis is specific in a degree comparable to the complement fixation test, and is sensitive enough for routine laboratory use, its sensitivity being somewhat higher than that of the complement fixation test. The Sabin–Feldman dye test gave positive results with human sera from cases clinically unrelated to toxoplasmosis much more frequently and usually in higher titers than did the complement fixation and the fluorescent antibody tests. On the other hand, the dye test showed a few negative results on sera for which the other tests proved to be positive. The indirect fluorescent antibody test for toxoplasmosis is safer and simpler to perform than the dye test, as living organisms are not used in the test, and prepared smears of killed toxoplasma suspension on cover slips can be kept antigenically active in the frozen state for at least 6 months.The indirect fluorescent antibody test for toxoplasmosis is recommended for use in the serodiagnosis of toxoplasmosis either as a single test or as a supplementary test for checking complement fixation negative sera or the results obtained by the dye test.

Investigation of Anti-Toxoplasma gondii Antibodies in Cats of the Ankara Region of Turkey Using the Sabin-Feldman Dye Test and an Indirect Fluorescent Antibody Test

2008 ◽  
Vol 94 (4) ◽  
pp. 817-820 ◽  
Author(s):  
Ayşegül Taylan Özkan ◽  
Bekir Çelebi ◽  
Cahit Babür ◽  
Araceli Lucio-Forster ◽  
Dwight D. Bowman ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Indirect Fluorescent Antibody

scholarly journals The Search forIxodes damminiandBorrelia burgdorferiin Nova Scotia

1992 ◽  
Vol 3 (5) ◽  
pp. 224-230 ◽  
Author(s):  
Colin R Bell ◽  
Harold B Specht ◽  
B Ann Coombs
Keyword(s):  
Nova Scotia ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Dermacentor Variabilis ◽  
Small Sample ◽  
Enzyme Linked Immunosorbent Assay ◽  
Indirect Fluorescent Antibody ◽  
Migrating Birds ◽  
Sera Samples

Twenty-fourIxodes damminiticks (23 adults and one nymph) have been recovered in Nova Scotia since 1984. There has not been a systematic search for larvae and none has been identified. The recovery of the nymph from a road-killed yellow throat bird,Geothypis trichas,in late May 1990 supports the contention that migrating birds are bringing deer ticks into the province every spring. In March and April 1991, four adult deer ticks were identified, suggesting that these ticks had overwintered. These deer tick specimens indicate that it is possible thatI damminiis becoming established in Nova Scotia, if it is not already established. There has been no evidence for the existence ofBorrelia burgdorferiin the province. The spirochete was not cultured from 650Dermacentor variabilisticks, nor were antibodies detected in a small sample of feral rodents using an indirect fluorescent antibody test. A survey of 137 dog sera samples, analyzed by enzyme-linked immunosorbent assay, also proved negative. There has been no confirmed indigenous case of Lyme disease in Nova Scotia to date.

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