scholarly journals Identification of novel molecules and pathogenic pathways in primary biliary cirrhosis: cDNA array analysis of intrahepatic differential gene expression

Gut ◽  
2001 ◽  
Vol 49 (4) ◽  
pp. 565-576 ◽  
Author(s):  
N A Shackel
Keyword(s):  
Gene Expression ◽  
Primary Biliary Cirrhosis ◽  
Differential Gene Expression ◽  
Cdna Array ◽  
Biliary Cirrhosis ◽  
Array Analysis ◽  
Differential Gene

Differential Gene Expression Profiles in Stage I Primary Biliary Cirrhosis

2005 ◽  
Vol 100 (9) ◽  
pp. 2019-2030 ◽  
Author(s):  
Masao Honda ◽  
Hiroshi Kawai ◽  
Yukihiro Shirota ◽  
Taro Yamashita ◽  
Shuichi Kaneko
Keyword(s):  
Gene Expression ◽  
Primary Biliary Cirrhosis ◽  
Differential Gene Expression ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Stage I ◽  
Biliary Cirrhosis ◽  
Differential Gene

Differential gene expression profile in breast cancer-derived stromal fibroblasts

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 21075-21075
Author(s):  
C. F. Singer ◽  
D. Gschwantler-Kaulich ◽  
A. Fink-Retter ◽  
G. Hudelist ◽  
C. Haas ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Mrna Expression ◽  
Differential Gene Expression ◽  
Gene Expression Pattern ◽  
Cdna Array ◽  
Tumor Stroma ◽  
Normal Breast ◽  
Stromal Fibroblasts ◽  
Differential Gene

21075 Background: Breast cancer is chatacterized by malignant transformation of epithelial cells, but stromal cells also play an important role in tumorigenesis. While tumoral fibroblasts display unique phenotypical properties, it is unclear whether they also represent are a specific subpopulation. Materials and Methods: Stromal fibroblasts deriving from malignant tissue of 10 women with invasive breast cancer, and from normal breast tissue of 10 women with benign breast disorders, were subjected to differential complementary DNA Microarray Analysis by using a 2400 gene cDNA array. Gene expression results were validated by real-time PCR and by immunohistochemistry. Results: In a cDNA Array that allows to analyze the differential gene expression of more than 2400 genes, the mRNA expression of 135 genes were increased more than 2 fold in fibroblasts from malignant breast tumors. The majority of these genes encode tumor-promoting cytokines, transcription factors and cell-matrix associated proteins. The mRNA expression of 110 genes decreased to less than 0.5 fold. The remaining 2155 genes were not significantly altered. Immunohistochemistry for selected proteins performed on biopsies from breast cancer and normal breast tissues confirmed the clinical relevance of our findings. Conclusion: Breast cancer-derived stromal fibroblasts show a distinctive gene expression pattern that differentiates them from normal breast stroma. Our observation of increased expression of tumor promotion-associated genes even in the absence of adjacent malignant epithelium suggests that tumor stroma is comprised of a fibroblastic subpopulation that provides for a microenvironment which supports tumor growth and invasion. No significant financial relationships to disclose.

scholarly journals Differential gene expression in colon carcinoma cells and tissues detected with a cDNA array

1999 ◽  
Vol 82 (6) ◽  
pp. 868-874 ◽  
Author(s):  
Steffen Backert ◽  
Marcos Gelos ◽  
Ursula Kobalz ◽  
Marie-Luise Hanski ◽  
Christian B�hm ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colon Carcinoma ◽  
Differential Gene Expression ◽  
Cdna Array ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Differential Gene

Transcriptional profiling using a novel cDNA array identifies differential gene expression during porcine embryo elongation

2005 ◽  
Vol 71 (2) ◽  
pp. 129-139 ◽  
Author(s):  
So Hyun Lee ◽  
Shu-Hong Zhao ◽  
Justin C. Recknor ◽  
Dan Nettleton ◽  
Sarah Orley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Transcriptional Profiling ◽  
Cdna Array ◽  
Porcine Embryo ◽  
Differential Gene
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