How intestinal epithelial cells tolerise dendritic cells and its relevance to inflammatory bowel disease

Gut ◽  
2009 ◽  
Vol 58 (9) ◽  
pp. 1291-1299 ◽  
Author(s):  
M Shale ◽  
S Ghosh
Keyword(s):  
Inflammatory Bowel Disease ◽  
Dendritic Cells ◽  
Epithelial Cells ◽  
Bowel Disease ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Inflammatory Bowel

Liver X Receptor Exerts Anti-Inflammatory Effects in Colonic Epithelial Cells via ABCA1 and Its Expression Is Decreased in Human and Experimental Inflammatory Bowel Disease

2021 ◽  
Author(s):  
José Miranda-Bautista ◽  
Juan A Rodríguez-Feo ◽  
Marta Puerto ◽  
Beatriz López-Cauce ◽  
José M Lara ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Epithelial Cells ◽  
Bowel Disease ◽  
Target Gene ◽  
Nuclear Factor Kappa B ◽  
Intestinal Epithelial Cells ◽  
Nuclear Factor ◽  
Intestinal Epithelial ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

Abstract Background Liver X receptor (LXR) exerts anti-inflammatory effects in macrophages. The aim of this study was to explore the expression and function of LXR in the colonic epithelium under inflammatory conditions. Methods The expression of LXR was explored by Western blot and immunohistochemistry in colonic biopsies from patients diagnosed with inflammatory bowel disease (IBD) and control patients. In addition, LXR and its target gene expression were analyzed in the colon from interleukin (IL)-10-deficient (IL-10-/-) and wild-type mice. Caco-2 cells were pretreated with the synthetic LXR agonist GW3965 and further challenged with IL-1β, the expression of IL-8 and chemokine (C-C motif) ligand (CCL)-28 chemokines, the activation of mitogen-activated protein (MAP) kinases, and the nuclear translocation of the p65 subunit of nuclear factor kappa B was evaluated. Glibenclamide was used as an ABCA1 antagonist. Results We found that LXR expression was downregulated in colonic samples from patients with IBD and IL-10-/- mice. The nuclear positivity of LXR inversely correlated with ulcerative colitis histologic activity. Colonic IL-1β mRNA levels negatively correlated with both LXRα and LXRβ in the colon of IL-10-/- mice, where a decreased mRNA expression of the LXR target genes ABCA1 and FAS was shown. In addition, IL-1β decreased the expression of the LXR target gene ABCA1 in cultured intestinal epithelial cells. The synthetic LXR agonist GW3965 led to a decreased nuclear positivity of the p65 subunit of nuclear factor kappa B, a phosphorylation ratio of the p44-42 MAP kinase, and the expression of CCL-28 and IL-8 in IL-1β-stimulated Caco-2 cells. The pharmacological inhibition of ABCA1 increased the phosphorylation of p44-42 after GW3965 treatment and IL-1β stimulation. Conclusions The LXR-ABCA1 pathway exerts anti-inflammatory effects in intestinal epithelial cells and is impaired in the colonic mucosa of patients with IBD and IL-10-/- mice.

Sign in / Sign up

Export Citation Format

Share Document