scholarly journals T lymphocytes of the normal human cornea.

1987 ◽  
Vol 71 (5) ◽  
pp. 384-386 ◽  
Author(s):  
O F Scheiffarth ◽  
F H Stefani ◽  
N Gabriel ◽  
O E Lund
Keyword(s):  
T Lymphocytes ◽  
Human Cornea ◽  
Normal Human

scholarly journals T LYMPHOCYTES OF THE NORMAL HUMAN CORNEA

1988 ◽  
Vol 28 (4) ◽  
pp. 340
Author(s):  
Scheiffarth OF ◽  
Stefani F ◽  
Gabriel N ◽  
Lund O
Keyword(s):  
T Lymphocytes ◽  
Human Cornea ◽  
Normal Human

An analysis of in vivo hprt mutant frequency in circulating T-lymphocytes in the normal human population: a comparison of four datasets

1994 ◽  
Vol 313 (2-3) ◽  
pp. 227-247 ◽  
Author(s):  
Derek R. Robinson ◽  
Kevin Goodall ◽  
Richard J. Albertini ◽  
J.Patrick O'Neill ◽  
Barry Finette ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Human Population ◽  
Normal Human ◽  
Mutant Frequency

scholarly journals Characterization of normal human CD3+ CD5- and γδ T cell receptor positive T lymphocytes

2008 ◽  
Vol 80 (1) ◽  
pp. 114-121 ◽  
Author(s):  
E. F. SROUR ◽  
T. LEEMHUIS ◽  
L. JENSKI ◽  
R. REDMOND ◽  
D. FILLAK ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Γδ T Cell ◽  
Normal Human ◽  
Γδ T Cell Receptor

scholarly journals Involvement of P-glycoprotein in the transmembrane transport of interleukin-2 (IL-2), IL-4, and interferon-gamma in normal human T lymphocytes

Blood ◽  
1996 ◽  
Vol 88 (5) ◽  
pp. 1747-1754 ◽  
Author(s):  
J Drach ◽  
A Gsur ◽  
G Hamilton ◽  
S Zhao ◽  
J Angerler ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Interferon Gamma ◽  
T Cell Activation ◽  
Interleukin 2 ◽  
Rhodamine 123 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ifn Gamma ◽  
Human T Lymphocytes ◽  
P Glycoprotein ◽  
Normal Human

Abstract The physiological role of the multidrug resistance P-glycoprotein (P- gp), which is expressed by normal human T lymphocytes, is still largely unknown. To investigate whether or not P-gp is involved in the transport of cytokines, peripheral blood lymphocytes were stimulated with phytohemagglutinin (PHA) in the absence or presence of P-gp inhibitors, and concentrations of cytokines (interleukin-2 [IL-2], IL- 4, IL-6, interferon-gamma [IFN-gamma]) in the supernatants of these cultures were quantitated by enzyme-linked immunosorbent assay. P-gp inhibitors included verapamil (Ver), tamoxifen (Tmx), and the P-gp specific monoclonal antibody UIC2. Release of IL-2 was significantly suppressed by these inhibitors at concentrations that were also effective in blocking efflux of Rhodamine-123 from normal T lymphocytes. IL-2 mRNA expression in lymphocytes was not different between PHA control and the cultures with P-gp inhibitors. Ver and Tmx did not interfere with T-cell activation as determined by CD25 and CD69 expression. In a nonhematological model, the P-gp expressing HCT-8 adenocarcinoma cell line, exogenously added IL-2 was shown to exert an inhibitory effect on P-gp mediated Rhodamine-123 efflux. In addition, transepithelial transport of IL-2 by electrophysiologically tight and polarized HCT-8 monolayers was examined. A time-dependent flux of IL-2 across dense monolayers, which was partially inhibited by Ver, was observed. We also investigated whether or not P-gp inhibitors suppressed release of other cytokines produced by activated T cells (IL- 4, IL-6, IFN-gamma). Release of IL-4 and IFN-gamma was significantly inhibited by Ver, Tmx, and UIC2; however, release of IL-6 remained unaffected. These data show P-gp mediated transmembrane flux of IL-2 in T lymphocytes and HCT-8 cells. We conclude that P-gp participates in the transport of cytokines (IL-2, IL-4, and IFN-gamma) in normal peripheral T lymphocytes.

scholarly journals Association of proliferating cell nuclear antigen with cyclin-dependent kinases and cyclins in normal and transformed human T lymphocytes

Blood ◽  
1994 ◽  
Vol 84 (10) ◽  
pp. 3413-3421 ◽  
Author(s):  
A Szepesi ◽  
EW Gelfand ◽  
JJ Lucas
Keyword(s):  
T Cells ◽  
T Lymphocytes ◽  
Cell Line ◽  
Proliferating Cell Nuclear Antigen ◽  
Kinase Activity ◽  
Jurkat Cells ◽  
Nuclear Antigen ◽  
Human T Lymphocytes ◽  
Normal Human ◽  
Proliferating Cell

Abstract The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and appears to be needed for both DNA synthesis and DNA repair. It is present in low amount in resting normal human T lymphocytes and, upon mitogenic stimulation with phorbol dibutyrate and ionomycin, begins to increase in mid-G1 phase, approximately 12 to 15 hours before entry into S phase. PCNA continues to increase in amount throughout the cell cycle and remains high in proliferating cultures. PCNA was extracted from activated normal T cells and from the transformed T-lymphoblastoid cell line Jurkat by a method that recovered approximately 98% of total cellular PCNA but yet retained its associations with other proteins. PCNA immunoprecipitates possessed H1 histone kinase activity, which increased in parallel with increasing cellular content of PCNA. Both the cdc2 and cdk2 kinases were found associated with PCNA in normal T cells, in amounts consistent with detected kinase activity. The results indicate that PCNA is not an inhibitory molecule of cdk/cyclin activity. Both normal and transformed T cells contained PCNA in association with cdk2, cdk4, cdk5, and cdk6, with the amount of PCNA associated with these molecules increasing in the order listed. Relatively high amounts of PCNA were also found associated with cyclins D2 and D3, the major cyclin partners of cdk6 in T cells. Though detected in normal cells, PCNA/cdc2 complexes were present in exceedingly low amount, if at all, in Jurkat cells. This cell line appeared to contain more of nearly all of the cdk and cyclin molecules analyzed, but there seemed to be little difference in the patterns of association of these molecules with PCNA in the cell line as compared with normal human T cells.

Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of normal human CD4+ T-lymphocytes

Biochimie ◽  
2020 ◽  
Vol 174 ◽  
pp. 34-43
Author(s):  
Dmitry D. Zhdanov ◽  
Anna A. Plyasova ◽  
Vadim S. Pokrovsky ◽  
Marina V. Pokrovskaya ◽  
Svetlana S. Alexandrova ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Nuclease Activity ◽  
Normal Human

Definition of the Roles for Iron and Essential Fatty Acids in Cell Cycle Progression of Normal Human T Lymphocytes

1993 ◽  
Vol 204 (2) ◽  
pp. 260-267 ◽  
Author(s):  
Naohiro Terada ◽  
Reuven Or ◽  
Agota Szepesi ◽  
Joseph J. Lucas ◽  
Erwin W. Gelfand
Keyword(s):  
Cell Cycle ◽  
Fatty Acids ◽  
T Lymphocytes ◽  
Cell Cycle Progression ◽  
Essential Fatty Acids ◽  
Cycle Progression ◽  
Human T Lymphocytes ◽  
Normal Human ◽  
Definition Of

cAMP regulates IL-10 production by normal human T lymphocytes at multiple levels: A potential role for MEF2

2009 ◽  
Vol 46 (3) ◽  
pp. 345-354 ◽  
Author(s):  
Kassiani Liopeta ◽  
Stavroula Boubali ◽  
Laura Virgilio ◽  
George Thyphronitis ◽  
George Mavrothalassitis ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Potential Role ◽  
Human T Lymphocytes ◽  
Normal Human ◽  
Multiple Levels

Pattern of distribution of T lymphocytes, Langerhans cells and HLA-DR bearing cells in normal human oral mucosa

1985 ◽  
Vol 93 (6) ◽  
pp. 513-521 ◽  
Author(s):  
JESPER REIBEL ◽  
ERIK DABELSTEEN ◽  
BIRGIT KENRAD ◽  
KARSTEN BUSCHARD
Keyword(s):  
T Lymphocytes ◽  
Oral Mucosa ◽  
Langerhans Cells ◽  
Hla Dr ◽  
Normal Human
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