scholarly journals Comparative sensitivity of fluorescent antibody staining of conjunctival scrapings and irradiated McCoy cell culture for the diagnosis of hyperendemic trachoma.

1980 ◽  
Vol 64 (4) ◽  
pp. 276-278 ◽  
Author(s):  
S Darougar ◽  
R M Woodland ◽  
B R Jones ◽  
A Houshmand ◽  
H A Farahmandian
Keyword(s):  
Cell Culture ◽  
Fluorescent Antibody ◽  
Comparative Sensitivity ◽  
Antibody Staining ◽  
Fluorescent Antibody Staining

scholarly journals Chlamydia pneumoniae in a Free-Ranging Giant Barred Frog (Mixophyes iteratus) from Australia

1999 ◽  
Vol 37 (7) ◽  
pp. 2378-2380 ◽  
Author(s):  
Lee Berger ◽  
Kym Volp ◽  
Sarah Mathews ◽  
Rick Speare ◽  
Peter Timms
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
16S Rrna ◽  
Chlamydia Pneumoniae ◽  
Fluorescent Antibody ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Antibody Staining ◽  
Free Ranging ◽  
Fluorescent Antibody Staining

The koala biovar of Chlamydia pneumoniae was identified in lung tissue from a sick, free-ranging giant barred frog (Mixophyes iteratus) by using electron microscopy, C. pneumoniae-specific fluorescent-antibody staining, cell culture, and sequencing of the ompA, ompB and 16S rRNA genes. This is the first report of a chlamydial strain infecting both a homeotherm and a poikilotherm and only the fourth host (in addition to humans, koalas, and horses) to be naturally infected with this species of Chlamydia. The frog had severe, chronic, mononuclear pneumonia and nonregenerative anemia and pancytopenia.

scholarly journals Use of Fluorescent-Antibody Staining of Cytocentrifuge-Prepared Smears in Combination with Cell Culture for Direct Detection of Respiratory Viruses

1998 ◽  
Vol 36 (7) ◽  
pp. 2112-2114 ◽  
Author(s):  
Kirk M. Doing ◽  
Mary Ann Jerkofsky ◽  
Elaine G. Dow ◽  
Jo Ann Jellison
Keyword(s):  
Cell Culture ◽  
Direct Detection ◽  
Fluorescent Antibody ◽  
Respiratory Viruses ◽  
Antibody Staining ◽  
Fluorescent Antibody Staining

Over a 3-year period, 1,003 respiratory samples were collected and examined for selected respiratory viruses with cytocentrifuged prepared smears stained with fluorescently labeled antibodies (IFA) in conjunction with cell culture. IFA results were compared with results obtained by cell culture. Viruses were isolated or detected by direct means in 401 samples. Agreement between culture and IFA was 90%.

Ingestion and survival of Y. pseudotuberculosis in HeLa cells

1975 ◽  
Vol 21 (12) ◽  
pp. 1997-2007 ◽  
Author(s):  
Åke Bovallius ◽  
Gustaf Nilsson
Keyword(s):  
Cell Culture ◽  
Hela Cells ◽  
Yersinia Pseudotuberculosis ◽  
Fluorescent Antibody ◽  
Cell System ◽  
Viable Count ◽  
Linear Increase ◽  
Intracellular Bacteria ◽  
Antibody Staining ◽  
Upper Limit

HeLa cells were infected with Yersinia pseudotuberculosis for 0.5–3 h. Intracellular bacteria could then be demonstrated by three different techniques: viable count, fluorescent-antibody staining, and electron microscopy. Most of the bacteria seemed to be viable, since there was a good positive correlation (0.94) between viable and fluorescent bacteria. The bacterial uptake seemed to be mediated by a phagocytic-like procedure. The intracellular bacteria seemed to reside in vacuoles some of which increased in size as a function of time. The kinetics of infection was studied after addition of 107 or 109 bacteria per cell culture (2 × 106 cells). After a lag period of about 30 min there was a linear increase of intracellular bacteria, and this uptake proceeded for 1–2 h until most of the bacteria were ingested or an upper limit of ingested bacteria was reached. The upper limit was calculated to be a mean of 60 per infected cell in the cell culture. More than 90% of the cells could be infected and a reasonable number of the bacteria survive in the cells for at least 3 days, as demonstrated by the viable-count technique.The bacteria–cell system may be used to study, for example, the effect of antibiotics or antibodies on intracellular bacteria and pathogenicity of intracellular diseases.

Profilin is predominantly associated with monomeric actin in Acanthamoeba

1999 ◽  
Vol 112 (21) ◽  
pp. 3779-3790 ◽  
Author(s):  
D.A. Kaiser ◽  
V.K. Vinson ◽  
D.B. Murphy ◽  
T.D. Pollard
Keyword(s):  
Monoclonal Antibodies ◽  
Plasma Membrane ◽  
Fluorescent Antibody ◽  
Gel Filtration ◽  
Live Cells ◽  
A431 Cells ◽  
Antibody Staining ◽  
Large Particles ◽  
Biochemical Fractionation ◽  
Fluorescent Antibody Staining

We used biochemical fractionation, immunoassays and microscopy of live and fixed Acanthamoeba to determine how much profilin is bound to its known ligands: actin, membrane PIP(2), Arp2/3 complex and polyproline sequences. Virtually all profilin is soluble after gentle homogenization of cells. During gel filtration of extracts on Sephadex G75, approximately 60% of profilin chromatographs with monomeric actin, 40% is free and none voids with Arp2/3 complex or other large particles. Selective monoclonal antibodies confirm that most of the profilin is bound to actin: 65% in extract immunoadsorption assays and 74–89% by fluorescent antibody staining. Other than monomeric actin, no major profilin ligands are detected in crude extracts. Profilin-II labeled with rhodamine on cysteine at position 58 retains its affinity for actin, PIP(2) and poly-L-proline. When syringe-loaded into live cells, it distributes throughout the cytoplasm, is excluded from membrane-bounded organelles, and concentrates in lamellapodia and sites of endocytosis but not directly on the plasma membrane. Some profilin fluorescence appears punctate, but since no particulate profilin is detected biochemically, these spots may be soluble profilin between organelles that exclude profilin. The distribution of profilin in fixed human A431 cells is similar to that in amoebas. Our results show that the major pool of polymerizable actin monomers is complexed with profilin and spread throughout the cytoplasm.

scholarly journals Detection ofCryptosporidium molnariOocysts from Fish by Fluorescent-Antibody Staining Assays forCryptosporidiumspp. Affecting Humans

2011 ◽  
Vol 77 (5) ◽  
pp. 1878-1880 ◽  
Author(s):  
Rona Barugahare ◽  
Michelle M. Dennis ◽  
Joy A. Becker ◽  
Jan Šlapeta
Keyword(s):  
Ribosomal Dna ◽  
Fluorescent Antibody ◽  
Small Subunit ◽  
Rapid Diagnosis ◽  
Antibody Staining ◽  
Direct Fluorescent Antibody ◽  
Murray Cod ◽  
Diagnosis Of Infection ◽  
Formalin Fixed ◽  
Fluorescent Antibody Staining

ABSTRACTThree direct fluorescent-antibody staining assay kits for the detection of zoonoticCryptosporidiumspecies were used to detectCryptosporidium molnarifrom Murray cod, and the cryptosporidia were characterized by using small-subunit (SSU) ribosomal DNA (rDNA). To facilitate rapid diagnosis of infection, this study demonstrated that all three kits detected freshC. molnariand two kits detected formalin-fixed oocysts.

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