scholarly journals Inhibition of nuclear factor-kappa B activation attenuates hydrogen peroxide-induced cytotoxicity in human lens epithelial cells

2006 ◽  
Vol 91 (3) ◽  
pp. 369-371 ◽  
Author(s):  
X.-H. Jin ◽  
K. Ohgami ◽  
K. Shiratori ◽  
Y. Koyama ◽  
K. Yoshida ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Epithelial Cells ◽  
Nuclear Factor Kappa B ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Nuclear Factor ◽  
Human Lens Epithelial Cells ◽  
Nuclear Factor Kappa

scholarly journals The Role of Nuclear Factor of Activated T Cells 5 in Hyperosmotic Stress-Exposed Human Lens Epithelial Cells

2021 ◽  
Vol 22 (12) ◽  
pp. 6296
Author(s):  
Gyu-Nam Kim ◽  
Young-Sool Hah ◽  
Hyemin Seong ◽  
Woong-Sun Yoo ◽  
Mee-Young Choi ◽  
...  
Keyword(s):  
T Cells ◽  
Epithelial Cells ◽  
Hyperosmotic Stress ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Nuclear Factor ◽  
Activated T Cells ◽  
Interacting Protein ◽  
Human Lens Epithelial Cells

We investigated the role of nuclear factor of activated T cells 5 (NFAT5) under hyperosmotic conditions in human lens epithelial cells (HLECs). Hyperosmotic stress decreased the viability of human lens epithelial B-3 cells and significantly increased NFAT5 expression. Hyperosmotic stress-induced cell death occurred to a greater extent in NFAT5-knockout (KO) cells than in NFAT5 wild-type (NFAT5 WT) cells. Bcl-2 and Bcl-xl expression was down-regulated in NFAT5 WT cells and NFAT5 KO cells under hyperosmotic stress. Pre-treatment with a necroptosis inhibitor (necrostatin-1) significantly blocked hyperosmotic stress-induced death of NFAT5 KO cells, but not of NFAT5 WT cells. The phosphorylation levels of receptor-interacting protein kinase 1 (RIP1) and RIP3, which indicate the occurrence of necroptosis, were up-regulated in NFAT5 KO cells, suggesting that death of these cells is predominantly related to the necroptosis pathway. This finding is the first to report that necroptosis occurs when lens epithelial cells are exposed to hyperosmolar conditions, and that NFAT5 is involved in this process.

Ocimum sanctumextracts attenuate hydrogen peroxide induced cytotoxic ultrastructural changes in human lens epithelial cells

2009 ◽  
Vol 23 (12) ◽  
pp. 1734-1737 ◽  
Author(s):  
Nabanita Halder ◽  
Sujata Joshi ◽  
Tapas Chandra Nag ◽  
Radhika Tandon ◽  
Suresh Kumar Gupta
Keyword(s):  
Hydrogen Peroxide ◽  
Epithelial Cells ◽  
Ultrastructural Changes ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells
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