scholarly journals Assessment of the human corneal endothelium: in vivo Topcon SP2000P specular microscope versus ex vivo sambacornea eye bank analyser

2007 ◽  
Vol 91 (2) ◽  
pp. 265-266 ◽  
Author(s):  
G Thuret ◽  
N Deb-Joardar ◽  
C Manissolle ◽  
M. Zhao ◽  
M Peoch ◽  
...  
Keyword(s):  
Corneal Endothelium ◽  
Ex Vivo ◽  
Eye Bank ◽  
Human Corneal Endothelium ◽  
Specular Microscope

scholarly journals VISUAL PERCEPTION BASED AUTOMATIC RECOGNITION OF CELL MOSAICS IN HUMAN CORNEAL ENDOTHELIUMMICROSCOPY IMAGES

2011 ◽  
Vol 27 (1) ◽  
pp. 53 ◽  
Author(s):  
Yann Gavet ◽  
Jean-Charles Pinoli
Keyword(s):  
Visual Perception ◽  
Corneal Endothelium ◽  
Ex Vivo ◽  
Optical Microscope ◽  
Human Visual Perception ◽  
Human Corneal Endothelium ◽  
Automatic Methods ◽  
Ex Vivo Imaging

The human corneal endothelium can be observed with two types of microscopes: classical optical microscope for ex-vivo imaging, and specular optical microscope for in-vivo imaging. The quality of the cornea is correlated to the endothelial cell density and morphometry. Automatic methods to analyze the human corneal endothelium images are still not totally efficient. Image analysis methods that focus only on cell contours do not give good results in presence of noise and of bad conditions of acquisition. More elaborated methods introduce regional informations in order to performthe cell contours completion, thus implementing the duality contour-region. Their good performance can be explained by their connections with several basic principles of human visual perception (Gestalt Theory and Marr's computational theory).

scholarly journals Regenerative capacity of the corneal transition zone for endothelial cell therapy

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Nicole Ming Sie ◽  
Gary Hin-Fai Yam ◽  
Yu Qiang Soh ◽  
Matthew Lovatt ◽  
Deepinder Dhaliwal ◽  
...  
Keyword(s):  
Transition Zone ◽  
Corneal Endothelium ◽  
Ex Vivo ◽  
Corneal Transplantation ◽  
Corneal Stroma ◽  
Regenerative Capacity ◽  
In Vivo Models ◽  
Endothelial Progenitors ◽  
Donor Corneas

AbstractThe corneal endothelium located on the posterior corneal surface is responsible for regulating stromal hydration. This is contributed by a monolayer of corneal endothelial cells (CECs), which are metabolically active in a continuous fluid-coupled efflux of ions from the corneal stroma into the aqueous humor, preventing stromal over-hydration and preserving the orderly arrangement of stromal collagen fibrils, which is essential for corneal transparency. Mature CECs do not have regenerative capacity and cell loss due to aging and diseases results in irreversible stromal edema and a loss of corneal clarity. The current gold standard of treatment for this worldwide blindness caused by corneal endothelial failure is the corneal transplantation using cadaveric donor corneas. The top indication is Fuchs corneal endothelial dystrophy/degeneration, which represents 39% of all corneal transplants performed. However, the global shortage of transplantable donor corneas has restricted the treatment outcomes, hence instigating a need to research for alternative therapies. One such avenue is the CEC regeneration from endothelial progenitors, which have been identified in the peripheral endothelium and the adjacent transition zone. This review examines the evidence supporting the existence of endothelial progenitors in the posterior limbus and summarizes the existing knowledge on the microanatomy of the transitional zone. We give an overview of the isolation and ex vivo propagation of human endothelial progenitors in the transition zone, and their growth and differentiation capacity to the corneal endothelium. Transplanting these bioengineered constructs into in vivo models of corneal endothelial degeneration will prove the efficacy and viability, and the long-term maintenance of functional endothelium. This will develop a novel regenerative therapy for the management of corneal endothelial diseases.

Automated in vivo and online morphometry of human corneal endothelium

1993 ◽  
Vol 31 (4) ◽  
pp. 432-437 ◽  
Author(s):  
G. Corkidi ◽  
J. Marquez ◽  
R. Usisima ◽  
R. Toledo ◽  
J. Valdez ◽  
...  
Keyword(s):  
Corneal Endothelium ◽  
Human Corneal Endothelium

Transplantation of Adult Human Corneal Endothelium Ex Vivo

Cornea ◽  
2001 ◽  
Vol 20 (7) ◽  
pp. 731-737 ◽  
Author(s):  
Ko-Hua Chen ◽  
Dimitri Azar ◽  
Nancy C. Joyce
Keyword(s):  
Corneal Endothelium ◽  
Ex Vivo ◽  
Human Corneal Endothelium ◽  
Adult Human

Fabrication of optical mosaics mimicking human corneal endothelium for the training and assessment of eye bank technicians

2011 ◽  
Vol 37 (1) ◽  
pp. 22 ◽  
Author(s):  
Manuel Flury ◽  
Zhiguo He ◽  
Nelly Campolmi ◽  
Philippe Gain ◽  
Bernard Kress ◽  
...  
Keyword(s):  
Corneal Endothelium ◽  
Eye Bank ◽  
Human Corneal Endothelium

scholarly journals Evaluation of reconstructed human corneal endothelium sheets made with porcine Descemet's membrane in vitro and in vivo

2020 ◽  
Vol 197 ◽  
pp. 108125 ◽  
Author(s):  
Qing Lu ◽  
Rong-Mei Peng ◽  
Na Feng ◽  
Ming-Da Wen ◽  
Lin- Hui He ◽  
...  
Keyword(s):  
Corneal Endothelium ◽  
Human Corneal Endothelium ◽  
Descemet's Membrane ◽  
Descemet’S Membrane
Sign in / Sign up

Export Citation Format

Share Document