scholarly journals Increased Ki-67 proliferative index and absence of P16INK4 in CIN-HPV related pathogenic pathways different from cervical squamous intraepithelial lesion

2006 ◽  
Vol 90 (7) ◽  
pp. 894-899 ◽  
Author(s):  
K-T Kuo ◽  
H-C Chang ◽  
C-H Hsiao ◽  
M-C Lin
Keyword(s):  
Squamous Intraepithelial Lesion ◽  
Proliferative Index ◽  
Ki 67 ◽  
Intraepithelial Lesion ◽  
Cervical Squamous Intraepithelial Lesion

scholarly journals HPV genotyping and p16/Ki-67 test significantly improve detection rate of high-grade cervical squamous intraepithelial lesion

2020 ◽  
Vol 16 (1) ◽  
pp. 87-93
Author(s):  
Piotr Lewitowicz ◽  
Anna Nasierowska-Guttmejer ◽  
Wojciech Rokita ◽  
Olga Adamczyk-Gruszka ◽  
Stanisław Gluszek ◽  
...  
Keyword(s):  
Detection Rate ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Ki 67 ◽  
Hpv Genotyping ◽  
Intraepithelial Lesion ◽  
Cervical Squamous Intraepithelial Lesion

scholarly journals Separation of Normal and Premalignant Cervical Epithelial Cells Using Confocal Light Absorption and Scattering Spectroscopic MicroscopyEx Vivo

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Ling Yang ◽  
Wen-Tao Liu ◽  
Hao Wu ◽  
Cheng Wang ◽  
Bo Ping ◽  
...  
Keyword(s):  
Light Absorption ◽  
Pap Smear ◽  
Precancerous Lesions ◽  
Scattered Light ◽  
Low Grade ◽  
Squamous Intraepithelial Lesion ◽  
Cervical Lesions ◽  
Pap Smear Test ◽  
Intraepithelial Lesion ◽  
Cervical Squamous Intraepithelial Lesion

Confocal light absorption and scattering spectroscopic (CLASS) microscopy can detect changes in biochemicals and the morphology of cells. It is therefore used to detect high-grade cervical squamous intraepithelial lesion (HSIL) cells in the diagnosis of premalignant cervical lesions. Forty cervical samples from women with abnormal Pap smear test results were collected, and twenty cases were diagnosed as HSIL; the rest were normal or low-grade cervical squamous intraepithelial lesion (LSIL). The enlarged and condensed nuclei of HSIL cells as viewed under CLASS microscopy were much brighter and bigger than those of non-HSIL cells. Cytological elastic scattered light data was then collected at wavelengths between 400 and 1000 nm. Between 600 nm to 800 nm, the relative elastic scattered light intensity of HSIL cells was higher than that of the non-HSIL. Relative intensity peaks occurred at 700 nm and 800 nm. CLASS sensitivity and specificity results for HSIL and non-HSIL compared to cytology diagnoses were 80% and 90%, respectively. This study demonstrated that CLASS microscopy could effectively detect cervical precancerous lesions. Further study will verify this conclusion before the method is used in clinic for early detection of cervical cancer.

scholarly journals Biomarker Expression in Multifocal Vulvar High-Grade Squamous Intraepithelial Lesions

Cancers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 5646
Author(s):  
Nikki B. Thuijs ◽  
Willemijn A. M. Schonck ◽  
Linde L. J. Klaver ◽  
Guus Fons ◽  
Marc van Beurden ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Expression Profiles ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Predictive Diagnostics ◽  
Ki 67 ◽  
Intraepithelial Lesion ◽  
Prospective Longitudinal ◽  
Intraepithelial Lesions ◽  
Six Genes

In patients with high-grade squamous intraepithelial lesion (HSIL) of the vulva, the presence of multiple lesions, called multifocal HSIL, is common. The aim of this exploratory study was to investigate biomarker expression profiles in multifocal HSIL. In total, 27 lesions from 12 patients with high-risk human papillomavirus (HPV)-positive multifocal HSIL were tested for HPV genotype, expression of p16INK4a and Ki-67, and DNA methylation of six genes. HPV16 was found most commonly in 21 (77.8%) HSILs. In two (16.4%) patients, HPV genotype differed between the lesions. All lesions demonstrated diffuse p16INK4a staining, of which three (11.1%) were combined with patchy staining. One patient (8.3%) demonstrated markedly different DNA methylation levels between lesions. Generally, heterogeneity in methylation profiles was observed between different patients, even when other biomarkers showed similar expression. In conclusion, this study is the first to demonstrate heterogeneity of individual lesions in patients with multifocal HSIL. The studied biomarkers have the potential to refine prognostic and predictive diagnostics. Future prospective, longitudinal studies are needed to further explore the potential of a biomarker profile for management of patients with multifocal HSIL.

scholarly journals The utility of p16INK4aand Ki-67 to identify high-grade squamous intraepithelial lesion in adolescents and young women

2012 ◽  
Vol 55 (3) ◽  
pp. 339 ◽  
Author(s):  
José Eleutério ◽  
DianeM Cavalcante ◽  
IaraM Linhares ◽  
MargaridaM.L Pompeu ◽  
PauloC Giraldo
Keyword(s):  
Young Women ◽  
High Grade ◽  
Squamous Intraepithelial Lesion ◽  
Ki 67 ◽  
Intraepithelial Lesion
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