scholarly journals Sodium hyaluronate (hyaluronic acid) promotes migration of human corneal epithelial cells in vitro

2004 ◽  
Vol 88 (6) ◽  
pp. 821-825 ◽  
Author(s):  
J A P Gomes
Keyword(s):  
Hyaluronic Acid ◽  
Epithelial Cells ◽  
Sodium Hyaluronate ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells

The Effect of Sodium Hyaluronate on the Growth of Rabbit Corneal Epithelial Cells In Vitro

1996 ◽  
Vol 12 (4) ◽  
pp. 409-415 ◽  
Author(s):  
TATSUYA MIYAZAKI ◽  
SATOSHI MIYAUCHI ◽  
TAKEHIKO NAKAMURA ◽  
SAWAKO TAKESHITA ◽  
KATSUYUKI HORIE
Keyword(s):  
Epithelial Cells ◽  
Sodium Hyaluronate ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial

scholarly journals Biocompatibility of Nanocellulose-Reinforced PVA Hydrogel with Human Corneal Epithelial Cells for Ophthalmic Applications

2019 ◽  
Vol 10 (3) ◽  
pp. 35 ◽  
Author(s):  
Tummala ◽  
Lopes ◽  
Mihranyan ◽  
Ferraz
Keyword(s):  
Epithelial Cells ◽  
Cell Viability ◽  
Protein Adsorption ◽  
Direct Contact ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Hydrogel Surface

Transparent composite hydrogel in the form of a contact lens made from poly(vinyl alcohol) (PVA) and cellulose nanocrystals (CNCs) was subjected to in vitro biocompatibility evaluation with human corneal epithelial cells (HCE-2 cells). The cell response to direct contact with the hydrogels was investigated by placing the samples on top of confluent cell layers and evaluating cell viability, morphology, and cell layer integrity subsequent to 24 h culture and removal of the hydrogels. To further characterize the lens–cell interactions, HCE-2 cells were seeded on the hydrogels, with and without simulated tear fluid (STF) pre-conditioning, and cell viability and morphology were evaluated. Furthermore, protein adsorption on the hydrogel surface was investigated by incubating the materials with STF, followed by protein elution and quantification. The hydrogel material was found to have affinity towards protein adsorption, most probably due to the interactions between the positively charged lysozyme and the negatively charged CNCs embedded in the PVA matrix. The direct contact experiment demonstrated that the physical presence of the lenses did not affect corneal epithelial cell monolayers in terms of integrity nor cell metabolic activity. Moreover, it was found that viable corneal cells adhered to the hydrogel, showing the typical morphology of epithelial cells and that such response was not influenced by the STF pre-conditioning of the hydrogel surface. The results of the study confirm that PVA-CNC hydrogel is a promising ophthalmic biomaterial, motivating future in vitro and in vivo biocompatibility studies.

scholarly journals Internalization of Staphylococcus aureus by Human Corneal Epithelial Cells: Role of Bacterial Fibronectin-Binding Protein and Host Cell Factors

2002 ◽  
Vol 70 (8) ◽  
pp. 4697-4700 ◽  
Author(s):  
Bradley D. Jett ◽  
Michael S. Gilmore
Keyword(s):  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Host Cell ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Viable Bacteria ◽  
Host Cell Factors ◽  
Human Corneal Epithelial Cells ◽  
Fibronectin Binding

ABSTRACT Wild-type Staphylococcus aureus was observed to be capable of invading human corneal epithelial cells (HCEC) in vitro. Internalization of S. aureus required expression of fibronectin-binding proteins (FnBPs); the capacity of an FnBP-deficient isogenic strain to invade HCEC was reduced by more than 99%. The binding of S. aureus to HCEC did not require viable bacteria, since UV-killed cells were observed to adhere efficiently. Invasion of HCEC by S. aureus involved active host cell mechanisms; uptake was nearly completely eliminated by cytochalasin D and genistein. These data suggest that FnBPs play a key role in host-parasite interactions and may serve as an important adhesin or invasin in ulcerative keratitis caused by S. aureus.

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