scholarly journals Effect of sulphasalazine and sulphapyridine on neutrophil superoxide production: role of cytosolic free calcium.

1990 ◽  
Vol 49 (5) ◽  
pp. 296-300 ◽  
Author(s):  
L Kanerud ◽  
I Hafstrom ◽  
B Ringertz
Keyword(s):  
Superoxide Production ◽  
Free Calcium ◽  
Cytosolic Free Calcium ◽  
Neutrophil Superoxide

scholarly journals Regulation of cytosolic free calcium concentration by intrasynaptic mitochondria.

1992 ◽  
Vol 3 (2) ◽  
pp. 235-248 ◽  
Author(s):  
A Martínez-Serrano ◽  
J Satrústegui
Keyword(s):  
Calcium Concentration ◽  
Adenine Nucleotides ◽  
Free Calcium ◽  
Nerve Terminals ◽  
Cytosolic Free Calcium ◽  
Free Calcium Concentration ◽  
Cytosolic Free Calcium Concentration ◽  
Presynaptic Nerve Terminals

By the use of digitonin permeabilized presynaptic nerve terminals (synaptosomes), we have found that intrasynaptic mitochondria, when studied "in situ," i.e., surrounded by their cytosolic environment, are able to buffer calcium in a range of calcium concentrations close to those usually present in the cytosol of resting synaptosomes. Adenine nucleotides and polyamines, which are usually lost during isolation of mitochondria, greatly improve the calcium-sequestering activity of mitochondria in permeabilized synaptosomes. The hypothesis that the mitochondria contributes to calcium homeostasis at low resting cytosolic free calcium concentration ([Ca2+]i) in synaptosomes has been tested; it has been found that in fact this is the case. Intrasynaptic mitochondria actively accumulates calcium at [Ca2+]i around 10(-7) M, and this activity is necessary for the regulation of [Ca2+]i. When compared with other membrane-limited calcium pools, it was found that depending on external concentration the calcium pool mobilized from mitochondria is similar or even greater than the IP3- or caffeine-sensitive calcium pools. In summary, the results presented argue in favor of a more prominent role of mitochondria in regulating [Ca2+]i in presynaptic nerve terminals, a role that should be reconsidered for other cellular types in light of the present evidence.

The role of cytosolic free calcium in the regulation of pyruvate dehydrogenase in synaptosomes

1994 ◽  
Vol 19 (1) ◽  
pp. 89-95 ◽  
Author(s):  
H. M. Huang ◽  
L. Toral-Barza ◽  
K. F. R. Sheu ◽  
G. E. Gibson
Keyword(s):  
Pyruvate Dehydrogenase ◽  
Free Calcium ◽  
Cytosolic Free Calcium

Role of cytosolic free calcium in the reorientation of pollen tube growth

1994 ◽  
Vol 5 (3) ◽  
pp. 331-341 ◽  
Author(s):  
Rui Malho ◽  
Nick D. Read ◽  
M. Salome Pais ◽  
Anthony J. Trewavas
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Free Calcium ◽  
Cytosolic Free Calcium

Contribution of CD54 to human eosinophil and neutrophil superoxide production

2001 ◽  
Vol 91 (2) ◽  
pp. 613-622 ◽  
Author(s):  
Shuji Takashi ◽  
Yoshio Okubo ◽  
Shiro Horie
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometric Analysis ◽  
Superoxide Production ◽  
Human Eosinophil ◽  
Flow Cytometric ◽  
Leukocyte Aggregation ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Neutrophil Superoxide

We have reported that CD54 on eosinophils is involved in eosinophil degranulation. However, the role of CD54 in eosinophil and neutrophil superoxide production is still uncertain. We assessed the effect of CD54 on eosinophils and neutrophils in recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF)- or phorbol myristate acetate (PMA)-induced superoxide production through CD18. Anti-CD54 monoclonal antibody attenuated leukocyte aggregation and superoxide production of rGM-CSF- or PMA-stimulated neutrophils and PMA-stimulated eosinophils. Anti-CD18 monoclonal antibody or theophylline attenuated superoxide production of eosinophils and neutrophils stimulated by either stimuli. Flow cytometric analysis demonstrated CD54 expression on freshly isolated neutrophils but not on freshly isolated eosinophils. CD54 newly expressed on eosinophils reached its peak expression 30 min after PMA stimulation. The increase in CD18 and CD54 expression on neutrophils caused by rGM-CSF stimulation was partially inhibited by theophylline. These data demonstrated that CD54 and CD18 interaction of eosinophils or neutrophils is involved in superoxide production and that the inhibition of superoxide production by theophylline may be at least partly due to the inhibition of CD54 and CD18.

Cytosolic Free Calcium and the Cytoskeleton in the Control of Leukocyte Chemotaxis

1998 ◽  
Vol 78 (4) ◽  
pp. 949-967 ◽  
Author(s):  
ELIZABETH J. PETTIT ◽  
FREDRIC S. FAY†
Keyword(s):  
Rational Design ◽  
Free Calcium ◽  
Molecular Signaling ◽  
Cytosolic Free Calcium ◽  
Kinase Activation ◽  
Pathogen Invasion ◽  
Kinase C ◽  
Leukocyte Chemotaxis ◽  
Essential Function

Pettit, Elizabeth J., and Frederic S. Fay. Cytosolic Free Calcium and the Cytoskeleton in the Control of Leukocyte Chemotaxis. Physiol. Rev. 78: 949–967, 1998. — In response to a chemotactic gradient, leukocytes extravasate and chemotax toward the site of pathogen invasion. Although fundamental in the control of many leukocyte functions, the role of cytosolic free Ca2+in chemotaxis is unclear and has been the subject of debate. Before becoming motile, the cell assumes a polarized morphology, as a result of modulation of the cytoskeleton by G protein and kinase activation. This morphology may be reinforced during chemotaxis by the intracellular redistribution of Ca2+stores, cytoskeletal constituents, and chemoattractant receptors. Restricted subcellular distributions of signaling molecules, such as Ca2+, Ca2+/calmodulin, diacylglycerol, and protein kinase C, may also play a role in some types of leukocyte. Chemotaxis is an essential function of most cells at some stage during their development, and a deeper understanding of the molecular signaling and structural components involved will enable rational design of therapeutic strategies in a wide variety of diseases.

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