scholarly journals Differential influence of p38 mitogen activated protein kinase (MAPK) inhibition on acute phase protein synthesis in human hepatoma cell lines

2005 ◽  
Vol 65 (7) ◽  
pp. 929-935 ◽  
Author(s):  
J Westra
Keyword(s):  
Protein Synthesis ◽  
Protein Kinase ◽  
Acute Phase Protein ◽  
Hepatoma Cell ◽  
Mitogen Activated Protein Kinase ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines ◽  
Phase Protein ◽  
Mitogen Activated Protein

scholarly journals Knockdown of Mitogen-Activated Protein Kinase Kinase 3 Negatively Regulates Hepatitis A Virus Replication

2021 ◽  
Vol 22 (14) ◽  
pp. 7420
Author(s):  
Tatsuo Kanda ◽  
Reina Sasaki-Tanaka ◽  
Ryota Masuzaki ◽  
Naoki Matsumoto ◽  
Hiroaki Okamoto ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Zinc Chloride ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Hepatoma Cell ◽  
Mitogen Activated Protein Kinase ◽  
Human Hepatoma Cell ◽  
Tlr Signaling ◽  
Hepatoma Cell Lines ◽  
Mitogen Activated Protein

Zinc chloride is known to be effective in combatting hepatitis A virus (HAV) infection, and zinc ions seem to be especially involved in Toll-like receptor (TLR) signaling pathways. In the present study, we examined this involvement in human hepatoma cell lines using a human TLR signaling target RT-PCR array. We also observed that zinc chloride inhibited mitogen-activated protein kinase 3 (MAP2K3) expression, which could downregulate HAV replication in human hepatocytes. It is possible that zinc chloride may inhibit HAV replication in association with its inhibition of MAP2K3. In that regard, this study set out to determine whether MAP2K3 could be considered a modulating factor in the development of the HAV pathogen-associated molecular pattern (PAMP) and its triggering of interferon-β production. Because MAP2K3 seems to play a role in antiviral immunity against HAV infection, it is a promising target for drug development. The inhibition of MAP2K3 may also prevent HAV patients from developing a severe hepatitis A infection.

scholarly journals Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction

2018 ◽  
Vol 19 (9) ◽  
pp. 2687 ◽  
Author(s):  
Tong-Hong Wang ◽  
Chuen Hsueh ◽  
Chin-Chuan Chen ◽  
Wan-Syuan Li ◽  
Chau-Ting Yeh ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Lines ◽  
Hepatoma Cell ◽  
Mitogen Activated Protein Kinase ◽  
Migration And Invasion ◽  
Small Rna Sequencing ◽  
Human Hepatoma Cell ◽  
Melatonin Treatment ◽  
Hepatoma Cell Lines ◽  
Mitogen Activated Protein

Melatonin is the main pineal hormone that relays light/dark-cycle information to the circadian system. Recent studies have examined the intrinsic antitumor activity of melatonin in various cancers, including hepatocellular carcinoma (HCC), the primary life-threatening malignancy in both sexes in Taiwan. However, the detailed regulatory mechanisms underlying melatonin’s anti-HCC activity remain incompletely understood. Here, we investigated the mechanisms by which the anti-HCC activity of melatonin is regulated. Human hepatoma cell lines were treated with 1 and 2 mM melatonin, and functional assays were used to dissect melatonin’s antitumor effect in HCC; small-RNA sequencing was performed to identify the microRNAs (miRNAs) involved in the anti-HCC activity of melatonin; and quantitative RT-PCR and Western blotting were used to elucidate how miRNAs regulate melatonin-mediated HCC suppression. Melatonin treatment at both doses strongly inhibited the proliferation, migration and invasion capacities of Huh7 and HepG2 cell lines, and melatonin treatment markedly induced the expression of the miRNA let7i-3p in cells. Notably, transfection of cells with a let7i-3p mimic drastically reduced RAF1 expression and activation of mitogen-activated protein kinase signaling downstream from RAF1, and rescue-assay results demonstrated that melatonin inhibited HCC progression by modulating let7i-3p-mediated RAF1 suppression. Our findings support the view that melatonin treatment holds considerable promise as a therapy for HCC.

Insulin modulation of acute-phase protein production in a human hepatoma cell line

Cytokine ◽  
1991 ◽  
Vol 3 (6) ◽  
pp. 619-626 ◽  
Author(s):  
Douglas Thompson ◽  
Stephen P. Harrison ◽  
Stuart W. Evans ◽  
John T. Whicher
Keyword(s):  
Cell Line ◽  
Acute Phase ◽  
Protein Production ◽  
Acute Phase Protein ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
Phase Protein

scholarly journals The Limonoids TS3 and Rubescin E Induce Apoptosis in Human Hepatoma Cell Lines and Interfere with NF-κB Signaling

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0160843 ◽  
Author(s):  
Nicole Lange ◽  
Armelle Tsamo Tontsa ◽  
Claudia Wegscheid ◽  
Pierre Mkounga ◽  
Augustin Ephrem Nkengfack ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatoma Cell ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Hepatoma Cell Lines
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