Transduction of peptides, proteins and nucleotides into live cells by cell penetrating peptides

2011 ◽  
Author(s):  
Franziska Mussbach ◽  
Martin Franke ◽  
Ansgar Zoch ◽  
Buerk Schaefer ◽  
Siegmund Reissmann
Keyword(s):  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Cell Penetrating

Transduction of peptides and proteins into live cells by cell penetrating peptides

2011 ◽  
Vol 112 (12) ◽  
pp. 3824-3833 ◽  
Author(s):  
Franziska Mussbach ◽  
Martin Franke ◽  
Ansgar Zoch ◽  
Buerk Schaefer ◽  
Siegmund Reissmann
Keyword(s):  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Cell Penetrating

scholarly journals Generation of Endosomolytic Reagents by Branching of Cell-Penetrating Peptides: Tools for the Delivery of Bioactive Compounds to Live Cells in Cis or Trans

2010 ◽  
Vol 21 (12) ◽  
pp. 2164-2167 ◽  
Author(s):  
Alfredo M. Angeles-Boza ◽  
Alfredo Erazo-Oliveras ◽  
Ya-Jung Lee ◽  
Jean-Philippe Pellois
Keyword(s):  
Bioactive Compounds ◽  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Cell Penetrating ◽  
In Cis

scholarly journals On the mechanisms of the internalization of S413-PV cell-penetrating peptide

2005 ◽  
Vol 390 (2) ◽  
pp. 603-612 ◽  
Author(s):  
Miguel Mano ◽  
Cristina Teodósio ◽  
Artur Paiva ◽  
Sérgio Simões ◽  
Maria C. Pedroso de Lima
Keyword(s):  
Cellular Uptake ◽  
Cell Membranes ◽  
Heparan Sulphate ◽  
Cell Penetrating Peptides ◽  
Membrane Receptors ◽  
Live Cells ◽  
Cell Penetrating Peptide ◽  
Cell Penetrating ◽  
Pv Cell

Cell-penetrating peptides have been shown to translocate across eukaryotic cell membranes through a temperature-insensitive and energy-independent mechanism that does not involve membrane receptors or transporters. Although cell-penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest both in vitro and in vivo, the mechanisms by which cellular uptake occurs remain unclear. In the face of recent reports demonstrating that uptake of cell-penetrating peptides occurs through previously described endocytic pathways, or is a consequence of fixation artifacts, we conducted a critical re-evaluation of the mechanism responsible for the cellular uptake of the S413-PV karyophilic cell-penetrating peptide. We report that the S413-PV peptide is able to accumulate inside live cells very efficiently through a rapid, dose-dependent and non-toxic process, providing clear evidence that the cellular uptake of this peptide cannot be attributed to fixation artifacts. Comparative analysis of peptide uptake into mutant cells lacking heparan sulphate proteoglycans demonstrates that their presence at the cell surface facilitates the cellular uptake of the S413-PV peptide, particularly at low peptide concentrations. Most importantly, our results clearly demonstrate that, in addition to endocytosis, which is only evident at low peptide concentrations, the efficient cellular uptake of the S413-PV cell-penetrating peptide occurs mainly through an alternative, non-endocytic mechanism, most likely involving direct penetration across cell membranes.

scholarly journals Relationships between Cargo, Cell Penetrating Peptides and Cell Type for Uptake of Non-Covalent Complexes into Live Cells

2013 ◽  
Vol 6 (2) ◽  
pp. 184-203 ◽  
Author(s):  
Andrea-Anneliese Keller ◽  
Franziska Mussbach ◽  
Reinhard Breitling ◽  
Peter Hemmerich ◽  
Buerk Schaefer ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Cell Type ◽  
Cell Penetrating

scholarly journals Delivery of SiC-based nanoparticles into live cells driven by cell-penetrating peptides SAP and SAP-E

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20498-20502 ◽  
Author(s):  
T. Serdiuk ◽  
I. Bakanovich ◽  
V. Lysenko ◽  
S. A. Alekseev ◽  
V. A. Skryshevsky ◽  
...  
Keyword(s):  
Functional Groups ◽  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Nanoparticle Delivery ◽  
Cell Penetrating ◽  
Covalent Conjugates

Cell-penetrating peptides enhance nanoparticle delivery into cells most efficiently if surface and peptide functional groups “match” to form non-covalent conjugates.

scholarly journals Identification of efficient prokaryotic cell-penetrating peptides with applications in bacterial biotechnology

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Hyang-Mi Lee ◽  
Jun Ren ◽  
Kha Mong Tran ◽  
Byeong-Min Jeon ◽  
Won-Ung Park ◽  
...  
Keyword(s):  
Genetic Manipulation ◽  
Marker Gene ◽  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Proof Of Concept ◽  
Delivery Method ◽  
E Coli ◽  
Efficient Delivery ◽  
Cell Penetrating ◽  
Gene Excision

AbstractIn bacterial biotechnology, instead of producing functional proteins from plasmids, it is often necessary to deliver functional proteins directly into live cells for genetic manipulation or physiological modification. We constructed a library of cell-penetrating peptides (CPPs) capable of delivering protein cargo into bacteria and developed an efficient delivery method for CPP-conjugated proteins. We screened the library for highly efficient CPPs with no significant cytotoxicity in Escherichia coli and developed a model for predicting the penetration efficiency of a query peptide, enabling the design of new and efficient CPPs. As a proof-of-concept, we used the CPPs for plasmid curing in E. coli and marker gene excision in Methylomonas sp. DH-1. In summary, we demonstrated the utility of CPPs in bacterial engineering. The use of CPPs would facilitate bacterial biotechnology such as genetic engineering, synthetic biology, metabolic engineering, and physiology studies.

scholarly journals Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore

2018 ◽  
Vol 115 (47) ◽  
pp. 11923-11928 ◽  
Author(s):  
Christoph Allolio ◽  
Aniket Magarkar ◽  
Piotr Jurkiewicz ◽  
Katarína Baxová ◽  
Matti Javanainen ◽  
...  
Keyword(s):  
Lipid Membrane ◽  
Cell Penetrating Peptides ◽  
Live Cells ◽  
Fusion Pore ◽  
Cell Penetration ◽  
Cell Penetrating ◽  
Penetration Mechanism ◽  
Dynamics Simulations ◽  
Kinetics Of ◽  
Molecular Picture

Arginine-rich cell-penetrating peptides do not enter cells by directly passing through a lipid membrane; they instead passively enter vesicles and live cells by inducing membrane multilamellarity and fusion. The molecular picture of this penetration mode, which differs qualitatively from the previously proposed direct mechanism, is provided by molecular dynamics simulations. The kinetics of vesicle agglomeration and fusion by an iconic cell-penetrating peptide—nonaarginine—are documented via real-time fluorescence techniques, while the induction of multilamellar phases in vesicles and live cells is demonstrated by a combination of electron and fluorescence microscopies. This concert of experiments and simulations reveals that the identified passive cell penetration mechanism bears analogy to vesicle fusion induced by calcium ions, indicating that the two processes may share a common mechanistic origin.

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