Voltammetric determination of flutamide and its metabolite 4-nitro-3-trifluoromethylaniline at a hanging mercury drop minielectrode

2011 ◽  
Vol 76 (12) ◽  
pp. 1811-1823 ◽  
Author(s):  
Karolina Pecková ◽  
Markéta Průchová ◽  
Josino C. Moreira ◽  
Jiří Barek ◽  
Jan Fischer ◽  
...  
Keyword(s):  
Solid Phase ◽  
Pharmaceutical Formulations ◽  
Differential Pulse ◽  
Voltammetric Determination ◽  
Phase Extraction ◽  
Optimum Conditions ◽  
Peak Potential ◽  
Main Metabolite ◽  
Limit Of Quantitation

Optimum conditions were found for direct current and differential pulse voltammetric determination of anticancer drug Flutamide and its main metabolite 4-nitro-3-trifluoromethylaniline on a hanging mercury drop minielectrode in mixed Britton–Robinson buffer (pH 12.0)–methanol (9:1) or 0.01MNaOH–methanol (9:1) media with limit of quantitation ca. 10–7mol l–1. It was proved that the newly developed method is applicable for the determination of Flutamide in pharmaceutical formulations and for the determination of both Flutamide and 4-nitro-3-trifluoromethylaniline in urine either directly with limit of quantitation ca. 10–5mol l–1or after solid phase extraction with limit of quantitation in the 10–7mol l–1concentration range. The sufficient peak potential difference of the two substances suggests the possibility of the analysis of their mixtures.

A combination of β-cyclodextrin functionalized magnetic graphene oxide nanoparticles with β-cyclodextrin-based sensor for highly sensitive and selective voltammetric determination of tetracycline and doxycycline in milk samples

RSC Advances ◽  
2016 ◽  
Vol 6 (48) ◽  
pp. 41675-41686 ◽  
Author(s):  
Amr A. Yakout ◽  
Deia Abd El-Hady
Keyword(s):  
Solid Phase ◽  
Differential Pulse ◽  
Voltammetric Determination ◽  
Phase Extraction ◽  
Carbon Paste ◽  
Magnetic Graphene Oxide ◽  
Selective Determination ◽  
Milk Samples ◽  
Highly Sensitive

Highly sensitive and selective determination of tetracycline and doxycycline in milk samples using solid phase extraction followed by differential pulse voltammetric determination on a β-cyclodextrin modified carbon paste sensor.

The voltammetric determination of 4-nitrobiphenyl

1991 ◽  
Vol 56 (3) ◽  
pp. 595-601 ◽  
Author(s):  
Jiří Barek ◽  
Gulamustafa Malik ◽  
Jiří Zima
Keyword(s):  
Differential Pulse Voltammetry ◽  
Differential Pulse ◽  
Voltammetric Determination ◽  
Hanging Mercury Drop Electrode ◽  
Optimum Conditions ◽  
Mercury Drop Electrode ◽  
Working Electrode ◽  
Pulse Voltammetry ◽  
Unstirred Solution

Optimum conditions were found for the determination of 4-nitrobiphenyl by fast scan differential pulse voltammetry at a hanging mercury drop electrode in the concentration range 1 . 10-5 to 2 . 10-7 mol l-1. A further increase in sensitivity was attained by adsorptive accumulation of this substance on the surface of the working electrode, permitting determination in the concentration range (2 – 10) . 10-8 mol l-1 with one minute accumulation of the substance in unstirred solution or (2 – 10) . 10-9 mol l-1 with three-minute accumulation in stirred solution. Linear scan voltammetry can be used to determine 4-nitrobiphenyl in the concentration range (2 – 10) . 10-9 mol l-1 with five-minute accumulation in stirred solution, with the advantage of a smoother baseline and smaller interference from substances that yield only tensametric peaks.

Determination of Diclofenac in Urine Samples by Molecularly-Imprinted Solid-Phase Extraction and Adsorptive Differential Pulse Voltammetry

2007 ◽  
Vol 19 (15) ◽  
pp. 1555-1561 ◽  
Author(s):  
Laura Fernández-Llano ◽  
M. Carmen Blanco-López ◽  
M. Jesús Lobo-Castañón ◽  
Arturo J. Miranda-Ordieres ◽  
Paulino Tuñón-Blanco
Keyword(s):  
Solid Phase Extraction ◽  
Differential Pulse Voltammetry ◽  
Solid Phase ◽  
Differential Pulse ◽  
Urine Samples ◽  
Phase Extraction ◽  
Molecularly Imprinted ◽  
Pulse Voltammetry

scholarly journals Simultaneous Determination of 23 Mycotoxins in Broiler Tissues by Solid Phase Extraction UHPLC-Q/Orbitrap High Resolution Mass Spectrometry

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 236
Author(s):  
Youyou Yang ◽  
Zhuolin He ◽  
Lei Mu ◽  
Yunfeng Xie ◽  
Liang Wang
Keyword(s):  
Mass Spectrometry ◽  
Solid Phase Extraction ◽  
Simultaneous Determination ◽  
Solid Phase ◽  
Harmful Effect ◽  
Phase Extraction ◽  
External Standard Method ◽  
Relative Standard ◽  
Limit Of Quantitation

Mycotoxins are a type of toxins harmful for not only animal but also human health. Cooccurrence of multi-mycotoxins could occur for food infected by several molds, producing multi-mycotoxins. It is necessary to develop corresponding determination methods, among which current mass spectrometry (MS) dominates. Currently, the accurate identification and quantitation of mycotoxins in complex matrices by MS with low resolution is still a challenge since false-positive results are typically obtained. Here, a method for the simultaneous determination of 23 mycotoxins in broiler tissues using ultra-high performance liquid chromatography-quadrupole/orbitrap HRMS was established. After the extraction by acetonitrile-water-formic acid (80:18:2, v/v/v), the purification by multifunctional purification solid phase extraction cartridges and the chromatographic separation on a C18 column, representative mycotoxins were determined by HRMS in full scan/data-dependent MS/MS acquisition mode. The quantitation was based on the external standard method. An MS/MS database of 23 mycotoxins was established to achieve qualitative screening and simultaneous quantification. Mycotoxins had a good linear relationship within a certain concentration range with correlation coefficients (r2) larger than 0.991 as well as the limit of quantitation of 1.80–300 μg/kg. The average recoveries at three different levels of low, medium and high fortification were 61–111% with relative standard deviations less than 13.5%. The method was fast, accurate, and suitable for the precise qualification of multiple mycotoxins in broiler tissues. 15 μg/kg zearalenone (ZEN) was detected in one liver sample among 30 samples from markets including chicken breast meat, liver, and gizzards. The result illustrated that the pollution of ZEN should not be neglected considering its harmful effect on the target organ of liver.

scholarly journals Determination of Residues of Alachlor and Related Herbicides in Crops by Liquid Chromatography with Electrochemical Detection

1997 ◽  
Vol 80 (5) ◽  
pp. 1104-1110 ◽  
Author(s):  
David A Nortrup
Keyword(s):  
Liquid Chromatography ◽  
Steam Distillation ◽  
Solid Phase ◽  
Base Hydrolysis ◽  
Phase Extraction ◽  
Test Portion ◽  
Strong Base ◽  
Relative Standard ◽  
Limit Of Quantitation

Abstract A method is described for determining residues of 3 acetamide herbicidesalachlor [2-chloro-N(2,6- diethylphenyl)-N(methoxymethyl)acetamide], acetochlor [2-chloro-N(2-ethyl-6-methylphenyl)-N (ethoxymethyl)acetamide), and butachlor [2-chloro-N(2,6-diethylphenyl)-N(butoxymethyl)- acetamide)by liquid chromatography (LC). Currently no published method determines metabolites from all 3 herbicides in crops. Strong-base hydrolysis after extraction of a test portion with water-acetonitrile results in the formation of 2,6- diethylaniline (DEA) and 2-(1-hydroxyethyl)-6-ethylaniline from alachlor metabolites, 2-ethyl-6-methylaniline and 2-(1-hydroxyethyl)-6-methylaniline from acetochlor metabolites, and DEA from butachlor. The anilines are isolated by steam distillation, partitioned with Supelclean ENVI-Chrom P solid-phase extraction columns, and methylated prior to LC analysis. The limit of quantitation of aniline moieties is about 0.01 ppm. Recoveries of 0.01- 0.40 ppm aniline moieties are generally 74-103%, with relative standard deviations of 2.2 to 14.0%.

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