Raman Optical Activity of the Central Part of Hinge Peptide

2005 ◽  
Vol 70 (4) ◽  
pp. 403-409 ◽  
Author(s):  
Josef Kapitán ◽  
Vladimír Baumruk ◽  
Vladimír Gut ◽  
Jan Hlaváček ◽  
Helena Dlouhá ◽  
...  
Keyword(s):  
Optical Activity ◽  
Disulfide Bonds ◽  
Vibrational Circular Dichroism ◽  
Helical Conformation ◽  
Disulfide Bridges ◽  
Polyproline Ii ◽  
Raman Optical Activity ◽  
Stretching Vibration ◽  
Human Igg1 ◽  
Cyclic Part

Central cyclic part of the hinge peptide (a parallel dimer of the pentapeptide Boc-Cys-Pro-Pro-Cys-Pro-NHCH3 with two disulfide bonds) derived from the sequence of human IgG1 is a rather rigid structure having predominantly polyproline II helical conformation as shown by vibrational circular dichroism spectra. It exhibits significant Raman optical activity (ROA) signal due to vibrations associated with the disulfide bridges. We report positive ROA for the S-S stretching vibration at 510 cm-1 and for the C-S stretching vibration at 655 cm-1. These signals can provide means to assess the conformation of disulfide bridges in proteins, otherwise difficult to investigate.

scholarly journals Raman optical activity study of poly-L-proline chains of various lengths

2010 ◽  
Vol 24 (3-4) ◽  
pp. 213-217 ◽  
Author(s):  
V. Profant ◽  
M. Šafarík ◽  
P. Bour ◽  
V. Baumruk
Keyword(s):  
Optical Activity ◽  
Conformational Stability ◽  
High Sensitivity ◽  
Wide Frequency Range ◽  
Peptide Chain ◽  
Helical Conformation ◽  
Polyproline Ii ◽  
Raman Optical Activity ◽  
Spectral Peaks ◽  
Chain Lengths

Raman and Raman optical activity (ROA) spectra of several oligo- and poly-L-proline samples of various chain lengths were measured in a wide frequency range between 120 and 1800 cm−1and analysed with respect to the main peptide chain conformation. Specifically, formation of polyproline II (PPII) helical conformation was studied in dependence on the increasing chain lengthNof the (L-proline)Nsample. Due to high sensitivity of the ROA technique to the conformational stability and rigidity of peptide chain we were able to determine the characteristic spectral peaks associated with formation of stable PPII helical conformation in studied systems. The most relevant peaks are located at 405, 535 and 945 cm−1. Additionally, based on our data analysis, we were able to determine the minimal length of (L-proline)Nchain necessary for creation of the stable PPII conformation asN= 6.

A combined Raman optical activity and vibrational circular dichroism study on artemisinin-type products

2020 ◽  
Vol 22 (32) ◽  
pp. 18014-18024 ◽  
Author(s):  
Jonathan Bogaerts ◽  
Filip Desmet ◽  
Roy Aerts ◽  
Patrick Bultinck ◽  
Wouter Herrebout ◽  
...  
Keyword(s):  
Density Functional Theory ◽  
Circular Dichroism ◽  
Dft Calculations ◽  
Optical Activity ◽  
Density Functional ◽  
Vibrational Circular Dichroism ◽  
Front Line ◽  
Functional Theory ◽  
Raman Optical Activity ◽  
Circular Dichroïsm

Artemisinin and two of its derivatives, dihydroartemisinin and artesunate, front line drugs against malaria, were studied using Raman optical activity (ROA), vibrational circular dichroism (VCD) experiments and density functional theory (DFT) calculations.

scholarly journals Determination of the Absolute Stereochemistry of Limonene and α-Santalol by Raman Optical Activity Spectroscopy

2012 ◽  
Vol 7 (4) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Akira Sakamoto ◽  
Nao Ohya ◽  
Toshio Hasegawa ◽  
Hiroaki Izumi ◽  
Nakako Tokita ◽  
...  
Keyword(s):  
Organic Compounds ◽  
Optical Activity ◽  
Absolute Configuration ◽  
Natural Compounds ◽  
Vibrational Circular Dichroism ◽  
Raman Optical Activity ◽  
The Absolute ◽  
Circular Dichroïsm ◽  
Absolute Stereochemistry

Determining the absolute stereochemistry of organic compounds in solution remains a challenge. We investigated the use of Raman optical activity (ROA) spectroscopy to address this problem. The absolute configurations of (+)-( R)- and (-)-( S)-limonene were determined by ROA spectroscopy, which can be applied to smaller amounts of sample as compared with vibrational circular dichroism (VCD) spectroscopy. This ROA method was also applied to (+)-( E)-α-santalol and shown to be successful in the determination of the absolute configuration of this compound. ROA spectroscopy shows promise as a useful tool for determining the absolute stereochemistry of many natural compounds.

Raman Optical Activity on a Solid Sample: Identification of Atropisomers of Perfluoroalkyl Chains Having a Helical Conformation and No Chiral Center

2019 ◽  
Vol 123 (18) ◽  
pp. 3985-3991 ◽  
Author(s):  
Takafumi Shimoaka ◽  
Masashi Sonoyama ◽  
Hideki Amii ◽  
Toshiyuki Takagi ◽  
Toshiyuki Kanamori ◽  
...  
Keyword(s):  
Optical Activity ◽  
Solid Sample ◽  
Chiral Center ◽  
Helical Conformation ◽  
Raman Optical Activity ◽  
Sample Identification
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