Comparison of Procedures for Isolation of Monoterpene Hydrocarbons from Fresh Needles of Picea abies and Picea omorica

2000 ◽  
Vol 65 (7) ◽  
pp. 1073-1081 ◽  
Author(s):  
Valerie Holubová ◽  
Iva Chvílíčková ◽  
Vlastimil Kubáň
Keyword(s):  
Picea Abies ◽  
Liquid Nitrogen ◽  
Steam Distillation ◽  
Extraction Efficiency ◽  
Total Content ◽  
Storage Conditions ◽  
Fresh Weight ◽  
Hexane Extraction ◽  
Monoterpene Hydrocarbons ◽  
And Storage

Extraction procedures (steam distillation, supercritical fluid extraction and solvent extraction) for isolation of monoterpene hydrocarbons from fresh needles of Picea abies and Picea omorica were optimised. The procedures were compared with the aim of minimizing consumption of needles and improving the extraction efficiency and repeatability. An influence of homogenisation procedures and storage conditions (liquid nitrogen, -18 and 4 °C) on the total content and composition of essential oils was studied. Cryogenic grinding (liquid nitrogen) combined with the extraction with cold hexane (extraction time 2 h) and subsequent GC-MS determination in freshly homogenised needles gives the best results (1.5-4 times better extraction efficiency, RSD < 10% for P. abies and < 25% for P. omorica). Limits of detections (3 S/N) for individual monoterpene hydrocarbons from units to tens of ng/g and recoveries 97.2-101.4% were found in fresh needles (calculated to fresh weight). While cooling to 4 °C is unacceptable, freezing at -18 °C for the period of 18 days in the dark gives also good results.

scholarly journals Optimizing Viable CD34 + Enumeration in Cryopreserved HPC Samples for Implementation of an External QA Program - Relevance to the Current Pandemic

Blood ◽  
2021 ◽  
Vol 138 (Supplement 1) ◽  
pp. 4847-4847
Author(s):  
Scott J Ragg ◽  
Annabella Chang ◽  
David Ma
Keyword(s):  
Liquid Nitrogen ◽  
Conflicts Of Interest ◽  
Cost Effective ◽  
Storage Conditions ◽  
Dry Ice ◽  
Cd34 Cells ◽  
Significant Difference ◽  
Mode Of Transport ◽  
Cost Implications ◽  
And Storage

Abstract Background The current External Quality Assurance (EQA) programs for haemopoietic progenitor cell (HPC) involve enumeration of total CD34 + cells in fixed samples, which does not align with clinical practice where analysis of viable CD34 + cells (vCD34 +) is required. The COVID-19 pandemic forced a fundamental change in the global procurement of allogeneic HPC for transplantation. To better meet the emergent challenges of transporting cryopreserved allogeneic HPC during pandemics, there is an urgent need for EQA programs to evaluate reproducibility and harmonization of vCD34 + HPC enumeration between collection and transplant centres. A successful vCD34 + EQA program will require cost-effective distribution of cryopreserved reference samples (CRS) with acceptable reproducibility and specificity. This study aims to evaluate the feasibility of distribution of CRS to participating facilities for vCD34 + enumeration using dry ice, instead of liquid nitrogen which is not suitable for CRS transport due to logistical and cost implications. Method: A 15 ml sample was cryopreserved from each of 10 HPC harvests from consented transplant donors (SVH HREC approval #10/07). Cryopreserved HPC samples were either stored on dry ice for 1-4 days, or on dry ice for one day followed by liquid nitrogen (LN 2) storage for 1-3 days to assess optimal conditions for vCD34 + EQA. For viable CD34 + measurement by flow cytometry, the single platform assay was performed using Trucount tubes containing CD45-FITC/CD34-PE and 7-AAD viability exclusion dye. The optimum transportation condition was validated in pilot and multi-center national studies which involved transport of CRS on dry ice to 12 recipient centers in 5 of the 6 Australian states. Results: Dry ice and LN 2 transport and storage conditions were simulated at the central laboratory and the vCD34 + enumerated. It was found that a combination of one day on dry ice followed by LN 2 storage stabilized the viability compared to continuous storage on dry ice. A successful pilot study confirmed the effect on vCD34 + count of shipping two CRS from central Lab to two interstate laboratories. For the national multicenter study, the transportation distances ranged from 0.5 - 4,000 km (median 513 km) with transit times ranging from 1- 26 hours (median 22.5 hours). Eight of 12 centers (67%) returned comparable results that were within ±10% of the median. There was no significant difference between samples tested immediately upon arrival or after subsequent LN 2 storage (p=0.41). There was no significant relationship between comparability of vCD34 + counts and the sample transit time (R=0.67, p=0.07) nor distance travelled (R = 0.19, p=0.55), showing that laboratory outcome was unrelated to sample transport. Conclusion: Dry ice distribution of cryopreserved HPC for up to 26 hours results in a stable CRS. The estimated cost of safer and more convenient dry ice delivery is &gt;20-fold lower than LN 2. This feasibility study illustrates that an EQA utilizing this mode of transport and storage of CRS is suitable for inter-facility harmonization and standardization forming the basis of an EQA programs for vCD34 + HPC enumeration. Disclosures No relevant conflicts of interest to declare.

scholarly journals Profiles of S-alk(en)ylcysteine sulfoxides in various garlic genotypes

2010 ◽  
Vol 28 (No. 4) ◽  
pp. 298-308 ◽  
Author(s):  
J. Horníčková ◽  
R. Kubec ◽  
K. Cejpek ◽  
J. Velíšek ◽  
J. Ovesná ◽  
...  
Keyword(s):  
Total Content ◽  
Storage Conditions ◽  
Climatic Conditions ◽  
Flowering Plant ◽  
Fresh Weight ◽  
The Czech Republic ◽  
Linear Discrimination ◽  
Pharmaceutical Industries ◽  
The Mean ◽  
The Individual

The contents of major S-alk(en)ylcysteine sulfoxides (namely alliin, methiin and isoalliin) were determined in a set of 58 various garlic genotypes (22 flowering plant morphotypes, 14 semi bolting plants and 22 scape absent morphotype plants), representing the garlic collection of the Allium gene bank in the Czech Republic. The plants were cultivated in four successive years (2005&ndash;2008) and analysed immediately after harvest and subsequently after eight weeks of storage at 5&deg;C. The total content of the three cysteine derivatives in fresh samples varied considerably between 3.35 mg/g fresh weigh and 12.77 mg/g fresh weight, with the mean of 7.50 mg/g fresh weight and the average relative proportions of alliin/methiin/isoalliin of 83/16/1. Upon 8-week storage at 5&deg;C, the average total amount of S-alk(en)ylcysteine sulfoxides increased by 30% to 9.75 mg/g fresh weight, with the alliin/methiin/isoalliin ratio changing to 82/14/4. The data obtained were statistically evaluated using linear discrimination analysis to distinguish the differences between the years of harvest, between freshly harvested and stored samples, and between the individual morphotypes. While the year-to-year differences between the samples were statistically not very significant, the fresh and stored samples as well as the individual garlic morphotypes differed considerably in S-alk(en)ylcysteine sulfoxide content. Our results indicate that the content of S-alk(en)ylcysteine sulfoxides primarily depends on various genetic factors and post-harvest storage conditions, whereas the climatic conditions during the growth (e.g. temperature, irrigation) influence their level to a lesser extent. Various implications for the food and pharmaceutical industries are discussed. &nbsp;

Cultivar and storage effects on germination and hard seed content of alfalfa

1999 ◽  
Vol 79 (2) ◽  
pp. 201-208 ◽  
Author(s):  
S. N. Acharya ◽  
D. G. Stout ◽  
B. Brooke ◽  
D. Thompson
Keyword(s):  
Medicago Sativa ◽  
Liquid Nitrogen ◽  
Storage Conditions ◽  
Temperature Treatment ◽  
Short Exposure ◽  
High Temperature Treatment ◽  
Hard Seed ◽  
Seed Content ◽  
Hard Seeds ◽  
And Storage

The impermeable coat of alfalfa (Medicago sativa L.) seed can reduce germination to an extent unacceptable for commercial use. The usual method of increasing germination of lots with high proportion of impermeable or hard seeds, mechanical scarification, can damage seeds. Experiments were conducted to determine the effect of cultivar, year of production and storage conditions on germination and hard seed content in alfalfa. Experiments with four Canadian cultivars indicated a significant effect of cultivar on seed weight, germination and hard seed content in freshly harvested seed. Year of production had a greater influence on these seed traits than cultivar. Under uncontrolled storage conditions, germination of 35 alfalfa synthetics increased and hard seed content decreased with time, although not at the same rate for all synthetics. Storage at 20 °C for up to 64 mo did not significantly decrease hard seed content. At 35 °C, hard seed content decreased continuously for all cultivars (for one cultivar to <5%) after 64 mo. Over 80.5 mo storage at room temperature (22 ± 2 °C), germination increased in a nonlinear fashion. Storing seed in sealed plastic bags at 35 °C delayed seed dehydration and the loss of hard seeds. Levels of nonviable seed were low (<10%), even after storage at 35 °C for 64 mo. Brief (1 min) exposure to liquid nitrogen increased germination of freshly harvested alfalfa seed to >90%. The results indicate that the germination percent of alfalfa can be increased and hard seed content reduced by short exposure to liquid nitrogen or storage at nonlethal high temperatures (35 °C) such that mechanical scarification may be unnecessary. Key words: Medicago sativa, hard seed, stored seed, seed dehydration, high temperature treatment, liquid nitrogen treatment

Barley seed storage under controlled conditions

2019 ◽  
pp. 140-150 ◽  
Author(s):  
O. A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov
Keyword(s):  
Hordeum Vulgare ◽  
Moisture Content ◽  
Spring Barley ◽  
Seed Storage ◽  
Growing Season ◽  
Storage Conditions ◽  
Meteorological Conditions ◽  
Hordeum Vulgare L ◽  
The Relationship ◽  
And Storage

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.

scholarly journals Changes of Physicochemical Properties of Starch Syrups Recommended for Winter Feeding of Honeybees during Storage

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 374
Author(s):  
Teresa Szczęsna ◽  
Ewa Waś ◽  
Piotr Semkiw ◽  
Piotr Skubida ◽  
Katarzyna Jaśkiewicz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Physicochemical Properties ◽  
Physicochemical Parameters ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Different Temperatures ◽  
And Storage ◽  
Bee Colonies ◽  
Temperature Time ◽  
Winter Feeding

The aim of this study was to determine the influence of storage temperature and time on physicochemical parameters of starch syrups recommended for the winter feeding of bee colonies. The studies included commercially available three starch syrups and an inverted saccharose syrup that were stored at different temperatures: ca. 20 °C, 10–14 °C, and ca. 4 °C. Physicochemical parameters of fresh syrups (immediately after purchase) and syrups after 3, 6, 9, 12, 15, 18, 21, and 24 months of storage at the abovementioned temperatures were measured. It was observed that the rate of unfavorable changes in chemical composition of starch syrups and the inverted saccharose syrup, mainly the changes in the 5-hydroxymethylfurfural (HMF) content, depended on the type of a syrup and storage conditions (temperature, time). Properties of tested starch syrups intended for winter feeding of bees stored at ca. 20 °C maintained unchanged for up to 6 months, whereas the same syrups stored at lower temperatures (10–14 °C) maintained unchanged physicochemical parameters for about 12 months. In higher temperatures, the HMF content increased. To date, the influence of this compound on bees has not been thoroughly investigated.

scholarly journals Stabilizing Grain Yield and Nutrition Quality in Purple Rice Varieties by Management of Planting Elevation and Storage Conditions

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 83
Author(s):  
Supapohn Yamuangmorn ◽  
Suchada Jumrus ◽  
Sansanee Jamjod ◽  
Narit Yimyam ◽  
Chanakan Prom-u-Thai
Keyword(s):  
Grain Yield ◽  
Antioxidant Capacity ◽  
Nutritional Value ◽  
Brown Rice ◽  
Storage Conditions ◽  
Harvest Management ◽  
Rice Varieties ◽  
Opposite Pattern ◽  
Dpph Activity ◽  
And Storage

Purple rice has become an interesting source of nutritional value among healthy cereal grains. The appropriate cultivation together with post-harvest management would directly benefit farmers and consumers. This study aimed (i) to determine the yield, grain nutritional quality, and antioxidant capacity of purple rice varieties grown at lowland and highland elevations, and (ii) to evaluate the effects of storage conditions on the stability of the rice nutritional value during six months of storage. The high anthocyanin PES variety grown in the lowlands had a higher grain yield than the plants grown in the highlands, but grain anthocyanin concentration had the opposite pattern. In the high antioxidant capacity KAK variety, grain yield and DPPH activity were not significantly different between plants grown at the two elevations. The storage of brown rice and vacuum-sealed packages were both found to preserve greater anthocyanin concentrations in PES, but there was no effect on the DPPH activity of KAK. The grain properties were not significantly different between storage at 4 °C and room temperature. This study suggests that the optimal cultivation practices and storage conditions would result in the higher yield and grain quality of purple rice varieties.

scholarly journals Evaluation of the Storage Conditions and Type of Cork Stopper on the Quality of Bottled White Wines

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 232
Author(s):  
María Consuelo Díaz-Maroto ◽  
Manuel López Viñas ◽  
Lourdes Marchante ◽  
María Elena Alañón ◽  
Ignacio Javier Díaz-Maroto ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Light Exposure ◽  
Total Content ◽  
Storage Conditions ◽  
Sensory Profile ◽  
Cork Stopper ◽  
White Wines ◽  
White Leds ◽  
Retail Outlets

The effects of different storage conditions, light exposure, temperature and different commercially available cork stoppers on the phenolic, volatile and sensorial profile of Verdejo wines were studied. Two natural corks of different visual quality and a microgranulated cork stopper were investigated over one year at two different storage conditions. One simulating light exposure and temperature in retail outlets and the other simulating optimal cellar conditions (darkness and 12 °C). The wines stored under commercial conditions showed greater losses of total and free SO2 and higher levels of brown-yellowish tones, related to the oxidation of flavan-3-ols. Although these wines underwent a decrease in the total content of stilbenes, a significant increase in trans-piceid was observed. In addition, these wines suffered important changes in their volatile and sensory profile. Volatile compounds with fruity and floral aromas decreased significantly, while volatile compounds related to aged-type characters, as linalool oxides, vitispirane, TDN or furan derivatives increased. Wines stored in darkness at 12 °C underwent minor changes and their sensory profiles were similar to wine before bottling. The high-quality natural corks and microgranulated corks better preserved the quality of the white wines from a sensory point of view. These results showed that temperature and light exposure conditions (diffuse white LEDs and 24 ± 2 °C) in retail outlets considerably decrease the quality of bottled white wines and, consequently, their shelf life, due to the premature development of aged-type characters.

scholarly journals Volatile Constituents of Trifolium Pratense and T. Repens from N.E. Italian Alpine Pastures

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aldo Tava ◽  
Daniele Ramella ◽  
Maris Grecchi ◽  
Paolo Aceto ◽  
Renato Paoletti ◽  
...  
Keyword(s):  
Steam Distillation ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Volatile Oil ◽  
Volatile Fraction ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Trifolium Pratense L

The composition of the volatile fraction of two important forage legumes from Italian sub-alpine N.E. pastureland, namely Trifolium pratense L. subsp. pratense (red clover) and T. repens subsp. repens (white clover) were investigated. The volatile oil was obtained from the fresh aerial parts by steam distillation and analyzed by GC/FID and GC/MS. The oil yield was 0.018 and 0.021% (weight/fresh weight basis) for T. pratense and T. repens, respectively. Several classes of compounds were found in both the oils, including alcohols, aldehydes, ketones, terpenes, esters, hydrocarbons, phenolics and acids. Qualitative and quantitative differences were found.

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