Synthesis of methylene blue modified 2'-deoxyuridine-5'-triphosphate and its use in DNA labeling reactions

1996 ◽  
Vol 61 (s1) ◽  
pp. 164-166
Author(s):  
Uwe Moller ◽  
Frank Schubert ◽  
Dieter Cech
Keyword(s):  
Methylene Blue ◽  
Dna Labeling

A SEM and Light Microscope Study of the Epidermal Leaf Structures of the Carnivorous Plant, Sarracenia purpurea, L.

1971 ◽  
Vol 29 ◽  
pp. 358-359
Author(s):  
B. J. Panessa ◽  
J. F. Gennaro
Keyword(s):  
Methylene Blue ◽  
Toluidine Blue ◽  
Outer Surface ◽  
Microscope Study ◽  
Light Microscope ◽  
Carnivorous Plant ◽  
Sarracenia Purpurea ◽  
Inner Surface

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.

Methylene blue reduces myocardial contractility

2012 ◽  
Vol 60 (S 01) ◽  
Author(s):  
H Weiler ◽  
O Moeller ◽  
M Wohlhoefer ◽  
LO Conzelmann ◽  
J Albers ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Myocardial Contractility

Modulation of endothelial inflammation after Methylene Blue: role of circulating cells

2014 ◽  
Vol 62 (S 01) ◽  
Author(s):  
I. Kanzler ◽  
F. Guo ◽  
N. Bogert ◽  
A. Moritz ◽  
A. Beiras-Fernandez
Keyword(s):  
Methylene Blue ◽  
Circulating Cells ◽  
Endothelial Inflammation

METHYLENE BLUE-MMX FOR SCREENING COLONOSCOPY

2019 ◽  
Author(s):  
A Repici ◽  
C Hassan ◽  
R Bisschops ◽  
P Bhandari ◽  
E Dekker ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Screening Colonoscopy

Using of modified-bentonite as low-cost sorbent for removal of methylene blue dye from aqueous solution

2020 ◽  
Vol 27 (2) ◽  
pp. 45-54
Author(s):  
Saraa Muwafaq Ibrahim ◽  
Ziad T. Abd Ali
Keyword(s):  
Aqueous Solution ◽  
Methylene Blue ◽  
Silanol Group ◽  
Infrared Spectrometry ◽  
Blue Dye ◽  
Ammonium Bromide ◽  
Vibration Band ◽  
Order Kinetic ◽  
Methylene Blue Dye ◽  
Modified Bentonite

Batch experiments have been studied to remove methylene blue dye (MB) from aqueous solution using modified bentonite. The modified bentonite was synthesized by replacing exchangeable calcium cations in natural bentonite with cationic surfactant cetyl trimethyl ammonium bromide (CTAB). The characteristics of modified bentonite were studied using different analysis such as Scanning electronic microscopy (SEM), Fourier transform infrared spectrometry (FTIR) and surface area. Where SEM shows the natural bentonite has a porous structure, a rough and uneven appearance with scattered and different block structure sizes, while the modified bentonite surface morphology was smooth and supplemented by a limited number of holes. On other hand, (FTIR) analysis that proved NH group aliphatic and aromatic group of MB and silanol group are responsible for the sorption of contaminate. The organic matter peaks at 2848 and 2930 cm-1 in the spectra of modified bentonite which are sharper than those of the natural bentonite were assigned to the CH2 scissor vibration band and the symmetrical CH3 stretching absorption band, respectively, also the 2930 cm-1 peak is assigned to CH stretching band. The batch study was provided the maximum removal efficiency (99.99 % MB) with a sorption capacity of 129.87 mg/g at specified conditions (100 mg/L, 25℃, pH 11 and 250rpm). The sorption isotherm data fitted well with the Freundlich isotherm model. The kinetic studies were revealed that the sorption follows a pseudo-second-order kinetic model which indicates chemisorption between sorbent and sorbate molecules.

Photocatalytic degradation of methylene blue from aqueous solutions using nanopillars-TiO2 thin films: Batch reactor studies

2018 ◽  
Vol 18 (3) ◽  
pp. 81-91 ◽  
Author(s):  
C. Lalhriatpuia
Keyword(s):  
Thin Films ◽  
Methylene Blue ◽  
Photocatalytic Activity ◽  
Organic Carbon ◽  
Photocatalytic Degradation ◽  
Batch Reactor ◽  
Tio2 Thin Films ◽  
Initial Concentration ◽  
Bet Specific Surface Area ◽  
Interfering Ions

Nanopillars-TiO2 thin films was obtained on a borosilicate glass substrate with (S1) and without (S2) polyethylene glycol as template. The photocatalytic behaviour of S1 and S2 thin films was assessed inthe degradation of methylene blue (MB) dye from aqueous solution under batch reactor operations. The thin films were characterized by the SEM, XRD, FTIR and AFM analytical methods. BET specific surface area and pore sizes were also obtained. The XRD data confirmed that the TiO2 particles are in its anatase mineral phase. The SEM and AFM images indicated the catalyst is composed with nanosized pillars of TiO2, evenly distributed on the surface of the substrate. The BET specific surface area and pore sizes of S1 and S2 catalyst were found to be 5.217 and 1.420 m2/g and 7.77 and 4.16 nm respectively. The photocatalytic degradation of MB was well studied at wide range of physico-chemical parameters. The effect of solution pH (pH 4.0 to 10.0) and MB initial concentration (1.0 to 10.0 mg/L) was extensively studied and the effect of several interfering ions, i.e., cadmium nitrate, copper sulfate, zinc chloride, sodium chloride, sodium nitrate, sodium nitrite, glycine, oxalic acid and EDTA in the photocatalytic degradation of MB was demonstrated. The maximum percent removal of MB was observed at pH 8.0 beyond which it started decreasing and a low initial concentration of the pollutant highly favoured the photocatalytic degradation using thin films and the presence of several interfering ions diminished the photocatalytic activity of thin films to some extent. The overall photocatalytic activity was in the order: S2 > S1 > UV. The photocatalytic degradation of MB was followed the pseudo-first-order rate kinetics. The mineralization of MB was studied with total organic carbon measurement using the TOC (total organic carbon) analysis.

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