Extracellular Polysaccharides of Candida mucifera Strain CCY 29-170-1

1996 ◽  
Vol 61 (3) ◽  
pp. 413-422
Author(s):  
Alžbeta Kardošová ◽  
Vladimír Pätoprstý
Keyword(s):  
Linkage Analysis ◽  
The Other ◽  
Extracellular Polysaccharides ◽  
Spectroscopic Methods ◽  
Malt Extract ◽  
Mild Acid Hydrolysis ◽  
Chain Unit ◽  
Polymeric Residue ◽  
Hydrolysis Of ◽  
Mild Acid

Extracellular polysaccharides produced by Candida mucifera strain CCY 29-170-1 in a liquid medium containing malt extract have been investigated by chemical, enzymic, and NMR spectroscopic methods. Mild acid hydrolysis of the native polysaccharide, followed by enzymic treatments of the polymeric residue, afforded two low-molecular neutral homopolysaccharides. The dominant one was a (1→4)-α-D-glucan with approximately each fifth chain unit branched on O-6. The other polysaccharide was a linear (1→4)-β-D-xylan. The results of linkage analysis evidenced that, originally, some xylan-chain units bore on O-3 substituents, which were split off during the hydrolytic steps.

scholarly journals Studies of the polysaccharide fraction from the lipopolysaccharide of Pseudomonas alcaligenes

1974 ◽  
Vol 139 (3) ◽  
pp. 633-643 ◽  
Author(s):  
James A. Lomax ◽  
George W. Gray ◽  
Stephen G. Wilkinson
Keyword(s):  
Gel Filtration ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Methylation Analysis ◽  
Mild Acid Hydrolysis ◽  
Pseudomonas Alcaligenes ◽  
Crude Polysaccharide ◽  
Hydrolysis Of ◽  
Mild Acid

Studies of the lipopolysaccharide of Pseudomonas alcaligenes strain BR 1/2 were extended to the polysaccharide moiety. The crude polysaccharide, obtained by mild acid hydrolysis of the lipopolysaccharide, was fractionated by gel filtration. The major fraction was the phosphorylated polysaccharide, for which the approximate proportions of residues were; glucose (2), rhamnose (0.7), heptose (2–3), galactosamine (1), alanine (1), 3-deoxy-2-octulonic acid (1), phosphorus (5–6). The heptose was l-glycero-d-manno-heptose. The minor fractions from gel filtration contained free 3-deoxy-2-octulonic acid, Pi and PPi. The purified polysaccharide was studied by periodate oxidation, methylation analysis, partial hydrolysis, and dephosphorylation. All the rhamnose and part of the glucose and heptose occur as non-reducing terminal residues. Other glucose residues are 3-substituted, and most heptose residues are esterified with condensed phosphate residues, possibly in the C-4 position. Free heptose and a heptosylglucose were isolated from a partial hydrolysate of the polysaccharide. The location of galactosamine in the polysaccharide was not established, but either the C-3 or C-4 position appears to be substituted and a linkage to alanine was indicated. In its composition, the polysaccharide from Ps. alcaligenes resembles core polysaccharides from other pseudomonads: no possible side-chain polysaccharide was detected.

Sequential Enzymatic and Mild-Acid Hydrolysis of By-Product of Carrageenan Process from Kappaphycus alvarezii

2019 ◽  
Vol 12 (2) ◽  
pp. 419-432 ◽  
Author(s):  
Fernando Roberto Paz-Cedeno ◽  
Eddyn Gabriel Solórzano-Chávez ◽  
Levi Ezequiel de Oliveira ◽  
Valéria Cress Gelli ◽  
Rubens Monti ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Kappaphycus Alvarezii ◽  
Mild Acid Hydrolysis ◽  
Hydrolysis Of ◽  
Mild Acid

scholarly journals IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS

1971 ◽  
Vol 133 (1) ◽  
pp. 39-52 ◽  
Author(s):  
Ten Feizi ◽  
Elvin A. Kabat ◽  
Giuseppe Vicari ◽  
Byron Anderson ◽  
W. Laurence Marsh
Keyword(s):  
Acid Hydrolysis ◽  
Blood Group ◽  
Ovarian Cyst ◽  
Periodate Oxidation ◽  
Human Saliva ◽  
Mild Acid Hydrolysis ◽  
Group B ◽  
Hydrolysis Of ◽  
Substantial Heterogeneity ◽  
Mild Acid

A partially purified blood group-like substance obtained from milk showed I activity with 2 of 21 anti-I sera. With these antisera, certain human ovarian cyst substances considered to be precursors of the A, B, H, Lea, and Leb substances also showed I activity comparable to the milk material. Strong I activity could be produced by one-stage periodate oxidation and Smith degradation of human ovarian cyst A and B substances, or of hog mucin A + H substance, or by mild acid hydrolysis of human saliva or ovarian cyst blood group B substance. The two sera indicate that I specificity appears at intermediate stages in the biosynthesis of the A, B, H, Lea, and Lea substances. Anti-I sera differ strikingly in their specificities, indicating substantial heterogeneity of the I determinants.

The Synthesis of Trisubstituted Enol Ethers and of Methyl Ketones from Ketones

1973 ◽  
Vol 51 (6) ◽  
pp. 981-983 ◽  
Author(s):  
Gilles Caron ◽  
Jean Lessard
Keyword(s):  
Acid Hydrolysis ◽  
Hydroxy Acid ◽  
Reliable Method ◽  
Acid Salt ◽  
Methyl Ketones ◽  
Enol Ether ◽  
Mild Acid Hydrolysis ◽  
Enol Ethers ◽  
Hydrolysis Of ◽  
Mild Acid

A reliable method for the synthesis of trisubstituted enol ethers (and of the corresponding methyl ketones) is described involving the condensation of the α-lithiated 2-methoxypropionic acid salt with a ketone to give a β-hydroxy acid, the cyclization to a β-lactone which is then decarboxylated (and mild acid hydrolysis of the enol ether).

Cis- and trans-2,4-dimethoxytetrahydropyran. Models for the study of the anomeric effect

1968 ◽  
Vol 46 (9) ◽  
pp. 1543-1548 ◽  
Author(s):  
F. Sweet ◽  
R. K. Brown
Keyword(s):  
Good Yield ◽  
Methoxy Group ◽  
Equilibrium Mixture ◽  
Anomeric Effect ◽  
Kcal Mole ◽  
Mild Acid Hydrolysis ◽  
Acid Catalyzed ◽  
Cis And Trans ◽  
Hydrolysis Of ◽  
Mild Acid

Acid-catalyzed methanolysis of 2-methoxy-5,6-dihydro-2H-pyran gave, in good yield, a 4.0:1.0 mixture of trans- and cis-2,4-dimethoxytetrahydropyran. Mild acid hydrolysis of 2-methoxy-5,6-dihydro-2H-pyran followed by acid-catalyzed reaction with methanol gave a cis-trans mixture of 4-hydroxy-2-methoxytetrahydropyran in very poor yield.From the equilibrium mixture of trans- and cis-2,4-dimethoxytetrahydropyran (4.0:1.0), the magnitude of the anomeric effect of the 2-methoxy group was calculated to be 1.4 kcal/mole.

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