Reverse-Phase High Performance Liquid Chromatography of Metal Chelates of 4-(2-Pyridylazo)resorcinol and 4-(2-Thiazolylazo)resorcinol. Simultaneous Determination of Low Concentrations of Co, Ni and Fe

1994 ◽  
Vol 59 (10) ◽  
pp. 2209-2226 ◽  
Author(s):  
Josef Doležal ◽  
Lumír Sommer
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Cetyltrimethylammonium Bromide ◽  
Ion Pair ◽  
Metal Chelates ◽  
Reverse Phase ◽  
Rp Hplc ◽  
Low Concentrations ◽  
Methanol Solutions

The behaviour of stable and inert metal chelates of 4-(2-pyridylazo)resorcinol and 4-(2-thiazolylazo)resorcinol in RP HPLC and in its ion-pair modification (IP RP HPLC) on Separon SGX RPS silica gel was studied. Good results were obtained by the ion-pair variant in water methanol solutions at pH 7 in the presence of cetyltrimethylammonium bromide. The technique proved to be convenient for the preconcentration, separation and quantitation of low concentrations of Fe, Co, and Ni in waters.

Development of reverse phase-high performance liquid chromatography (RP-HPLC) method for determination of selected antihypertensive active flavonoids (rutin, myricetin, quercetin, and kaempferol) in medicinal plants found in Botswana

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Katso Binang ◽  
David T. Takuwa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
High Performance ◽  
Zingiber Officinale ◽  
Hplc Method ◽  
Reverse Phase ◽  
Rp Hplc ◽  
Lippia Javanica

Abstract The aim of the study was to develop a rapid, efficient, and cheap chromatographic method for determining four selected antihypertensive active flavonoid compounds in medicinal plants in Botswana. The determination of rutin, quercetin, and kaempferol in selected medicinal plants was conducted in less than 6 min using the developed reverse phase-high performance liquid chromatography (RP-HPLC) method with a 2.7 µm Ascentis C18 express column (150 × 4.60 mm i.d) at 340, 360, and 368 nm detection wavelengths and mobile phase of methanol and 0.068% of formic acid solution in isocratic elution. Validation results showed good selectivity, linearity (r 2 > 0.99), high percentage recoveries (90.2–104.7%), and precision (% RSD < 2) for n = 3, confirming suitability of the method for determination of the investigated flavonoids in Zingiber officinale (ginger). Application of the developed RP-HPLC method was performed in selected medicinal plants (Lippia javanica ) (mosukujane), Myrothanmus flabellious (galalatshwene), and Elephantorrhiza elephantina (mositsana) used to manage hypertension by herbalists in Botswana. M. flabellious a very commonly used plant for managing hypertension was found to contain highest amounts of rutin and myricetin, whereas nothing was detected for E. elephantina.

Determination of simple bromophenols in marine fishes by reverse-phase high performance liquid chromatography (RP-HPLC)

Talanta ◽  
2005 ◽  
Vol 68 (2) ◽  
pp. 323-328 ◽  
Author(s):  
V DASILVA ◽  
M DACUNHAVELOSO ◽  
A DEOLIVEIRA ◽  
G SANTOS ◽  
P DEPPEREIRA ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Marine Fishes ◽  
Reverse Phase ◽  
Rp Hplc
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