On the Nature of the So-Called Methoxychelidonine

1994 ◽  
Vol 59 (2) ◽  
pp. 429-434 ◽  
Author(s):  
Jiří Slavík ◽  
Eva Táborská ◽  
Hana Bochořáková
Keyword(s):  
High Resolution ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Individual Compound ◽  
Chelidonium Majus ◽  
Mass Spectral ◽  
Hplc Technique ◽  
Liquid Chromatographic ◽  
Hplc Analyses ◽  
Resolution Mass

The so-called methoxychelidonine, an alkaloid isolated in 1924 from Chelidonium majus L. (Papaveraceae), is not an individual compound, but a mixture of three related alkaloids (+)-chelamine (III), (+)-homochelidonine (V) and (+)-chelamidine (IV) as it was deduced from high resolution mass spectral, thin-layer chromatographic and high-performance liquid chromatographic (HPLC) analyses of the authentic Gadamer's sample. The content of III, V and IV in "methoxychelidonine" found by HPLC technique was 67, 28, and 5 wt.%, respectively.

Liquid-chromatographic measurement of p-aminobenzoic acid and its metabolites in serum.

1988 ◽  
Vol 34 (11) ◽  
pp. 2235-2238 ◽  
Author(s):  
L L Yung-Jato ◽  
P R Durie ◽  
S J Soldin
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Peak Height ◽  
Individual Compound ◽  
Aminobenzoic Acid ◽  
Serum Samples ◽  
The Individual ◽  
A Minor ◽  
Liquid Chromatographic

Abstract This is a high-performance liquid-chromatographic method for measuring p-aminobenzoic acid (PABA) and its metabolites in plasma or serum. Samples are deproteinized, then extracted with organic solvents before chromatography. For quantification, the peak height of the individual compound is compared with that of the internal standard. Analytical recoveries ranged from 41% to 100%, depending on the compound studied. Comparison of patients' samples after oral administration of either N-benzoyl-L-tyrosyl-p-aminobenzoic acid or free PABA revealed that PABA is extensively metabolized and conjugated to either p-acetamidobenzoic acid, p-aminohippuric acid, or p-acetamidohippuric acid. PABA concentrations in serum as measured with the Bratton-Marshall ultraviolet spectrophotometric procedure would appear predominantly to reflect measurements of metabolites, with only a minor contribution from PABA itself.

scholarly journals Determination of Ketoconazole in tablets by using three different methods

2012 ◽  
Vol 4 ◽  
Author(s):  
Biljana Gjorgjeska
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Pharmaceutical Preparations ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Control Analysis ◽  
Analytical Work ◽  
Liquid Chromatographic ◽  
Hplc Analyses

Ketoconazole has been widely used as an antifungal drug  that is formulated as tablets, cream and over­the­counter ketoconazole shampoo. The aim of this research was to study and to standardize an ultraviolet spectrophotometric (UVS) method, potentiometric  and a high  performance  liquid  chromatographic  (HPLC) method  for  the determination  of ketoconazole in commercially available Oromycosal® tablets. These three methods were compared and discussed with respect to  their  sensitivity,  selectivity  and ready­applicability  in  routine  analytical  work.   Absorption  spectra and spectrophotometric determinations were carried out on the UVS spectrophotometer. Investigated concentrations were in range from  0.003 to  0.02mg?dm­3. The absorbance was measured at 224  nm.  In potentiometric  titrations  glass  and saturated (KCl) calomel electrode were used. HPLC analyses of ketoconazole were carried in the presence of econazole as internal standard. It can be concluded that the described methods are simple, fast and reliable for determination of ketoconazole in pharmaceutical preparations. The preparation of the samples is easy, the excipients do not  interfere in the methods, so they can be used in routine quality control analysis.

scholarly journals FLAVONOIDS AND ANTI-OXIDANT ACTIVITY MEDIATED GASTROPROTECTIVE ACTION OF LEATHERY MURDAH, TERMINALIA CORIACEA (ROXB.) WIGHT & ARN. LEAF METHANOLIC EXTRACT IN RATS

2017 ◽  
Vol 54 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Mohammed Safwan ALI KHAN ◽  
Shaaz NAZAN ◽  
Abdul Manan MAT JAIS
Keyword(s):  
Superoxide Dismutase ◽  
High Performance ◽  
Methanolic Extract ◽  
Ultra Violet ◽  
High Performance Liquid Chromatographic ◽  
Ulcer Index ◽  
Mass Spectral Analysis ◽  
Mass Spectral ◽  
Anti Oxidant ◽  
Liquid Chromatographic

ABSTRACT BACKGROUND Leathery Murdah, Terminalia coriacea (Roxb.) Wight & Arn. from family Combretaceae is used in Ayurveda and Siddha traditional systems of medicine to heal ulcers. OBJECTIVE The present study was conducted to assess the gastroprotective effect and understand the fundamental mechanism of action of Leathery Murdah, Terminalia coriacea (Roxb.) Wight & Arn. Leaf Methanolic Extract. METHODS The test extract was screened for anti-ulcer activity by Aspirin induced ulcerogenesis in pyloric ligation and ethanol induced gastric ulcers at three doses - 125, 250, and 500 mg/kg, p.o. using Ranitidine 50 mg/kg and Misoprostol 100 μg/kg as standard drug in respective models. Seven parameters were carefully examined, that is, ulcer index, total protein, mucin, catalase, malondialdehyde, and superoxide dismutase levels and histopathology. High Performance Liquid Chromatographic - Ultra Violet profiling and Liquid Chromatography - Mass Spectral analysis of crude Terminalia coriacea leaves methanolic extract were carried out as a part of chemical characterization to identify bioactive compounds. RESULTS All the test doses exhibited significant gastroprotective function, particularly the higher doses demonstrated improved action. The results revealed a significant increase in the levels of catalase, superoxide dismutase, and Mucin with reduction in ulcer index, the levels of total protein, and malondialdehyde. Histopathological observations also illustrated the gastroprotective effect of Terminalia coriacea leaves methanolic extract. CONCLUSION Terminalia coriacea leaves methanolic extract exhibited strong anti-oxidant and anti-secretory activities mediated gastroprotection besides inducing the gastric mucosal production. The observed pharmacological response can be attributed to the flavonoidal compounds namely - Quercetin-3-O-rutinoside, Luteolin-7-O-glucoside, Myricetin hexoside, Quercetin-3-O-glucoside, Isorhamnetin-3-O-rhamnosylglucoside and Isorhamnetin-3-O-glucoside identified in the extract for the first time with High Performance Liquid Chromatographic - Ultra Violet and Liquid Chromatography - Mass Spectral analysis.

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