Behaviour of Human Blood Serum Orosomucoid (Acid α1-Glycoprotein) in the Presence of Small Aliphatic Alcohols

1993 ◽  
Vol 58 (11) ◽  
pp. 2701-2714 ◽  
Author(s):  
Vladimír Karpenko ◽  
Ludmila Šinkorová ◽  
Ludmila Janáčková
Keyword(s):  
Volume Fraction ◽  
Structural Features ◽  
Uv Spectra ◽  
Optical Methods ◽  
Mathematical Basis ◽  
Helix Formation ◽  
Spectral Peaks ◽  
Electrostatic Contribution ◽  
The Stability ◽  
Α Helix

The influence of both methanol and ethanol on the stability of orosomucoid molecule was studied over a broad range of alcohol concentrations (volume fraction 0 - 70%) and pH between 7 and 12. The data obtained by optical methods were compared with information from prediction studies which were focused on the structural features and physicochemical parameters of amino acid in the orosomucoid molecule. From this analysis the following conclusions can be drawn: (i) ethanol exerts a more pronounced effect on α-helix formation than methanol does, (ii) at higher pH the electrostatic contribution is the crucial effect in the destabilization of the orosomucoid molecule, (iii) out of 12 phenylalanines in the orosomucoid molecule only 1 - 2 are exposed to the solvent; of the residues which are not subjected to substitution appears to be Phe 141, (iv) its neighbor, Tyr 142, appears to be a key residue, the dissociation of which destabilizes one of two longer helical segments of the orosomucoid molecule, (v) the limits of applicability of the four-derivatives of UV spectra with respect to phenylalanines were examined; analysis of the data is recommended on a more precise mathematical basis, taking into consideration the bandwidth of the spectral peaks.

scholarly journals Analysis of insertion mutants in distal α9 portion of C-terminal heptad repeat (CHR) of HIV-1 gp41 subunit

2020 ◽  
Author(s):  
Hongyun Wang ◽  
Jiping Song ◽  
Yasushi Kawaguchi ◽  
Jun-ichiro Inoue ◽  
Zene Matsuda
Keyword(s):  
Membrane Fusion ◽  
Underlying Mechanism ◽  
Heptad Repeat ◽  
Target Cells ◽  
Wild Type ◽  
Helix Formation ◽  
Fusion Inhibition ◽  
The Stability ◽  
Α Helix ◽  
Insertion Mutants

AbstractWe have made insertion mutants in α9 of HXB2 gp41 and observed similar phenotypes like recent JRFL mutants: insertion of alanine (653+A), but not glutamine (653+Q), severely attenuated membrane fusion. To understand the underlying mechanism, we performed the fusion inhibition assay by corresponding mutant C34 peptides. Both mutant C34 peptides added at the beginning of the coculture of the effector and target cells showed less efficient inhibition of membrane fusion, which was similar to wildtype C34 added after 30 min of coculture, indicating slow association of mutant C34 peptides with the N-terminal heptad region of gp41. Due to uninterpretable CD profiles of C34 and N36, we tested the longer peptide pairs (N46 and C42) and observed CD profiles indicative of weak α-helix formation. The melting temperatures for N46-C42 pairs of 653+A, 653+Q, and wild type were 56.8 °C, 59.8°C, and 96°C, respectively. Taken together, our data suggested that the phenotypic difference in membrane fusion between 653+A and 653+Q (or wild type) was not based on the stability of the six-helix bundle (6HB), but due to differences in the kinetics of 6HB formation. Further, we examined additional insertions (E, R, I, and L) at position 653, for which only I and L showed fusion recovery similar to Q, suggesting that the polar nature of glutamine was not a phenotypic determinant.

scholarly journals Quantifying the Effects of Vibration on Medicines in Transit Caused by Fixed-Wing and Multi-Copter Drones

Drones ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 22
Author(s):  
Andrew Oakey ◽  
Tim Waters ◽  
Wanqing Zhu ◽  
Paul G. Royall ◽  
Tom Cherrett ◽  
...  
Keyword(s):  
Road Transport ◽  
Uv Spectra ◽  
Frequency Spectra ◽  
Vibration Measurements ◽  
British Pharmacopoeia ◽  
Medical Products ◽  
Industry Standard ◽  
The Stability ◽  
In Transit

The concept of transporting medical products by drone is gaining a lot of interest amongst the medical and logistics communities. Such innovation has generated several questions, a key one being the potential effects of flight on the stability of medical products. The aims of this study were to quantify the vibration present within drone flight, study its effect on the quality of the medical insulin through live flight trials, and compare the effects of vibration from drone flight with traditional road transport. Three trials took place in which insulin ampoules and mock blood stocks were transported to site and flown using industry standard packaging by a fixed-wing or a multi-copter drone. Triaxial vibration measurements were acquired, both in-flight and during road transit, from which overall levels and frequency spectra were derived. British Pharmacopeia quality tests were undertaken in which the UV spectra of the flown insulin samples were compared to controls of known turbidity. In-flight vibration levels in both the drone types exceeded road induced levels by up to a factor of three, and predominant vibration occurred at significantly higher frequencies. Flown samples gave clear insulin solutions that met the British Pharmacopoeia specification, and no aggregation of insulin was detected.

ALPHA-HELIX FORMATION IN C-PEPTIDE RNASE-A INVESTIGATED BY PARALLEL TEMPERING SIMULATIONS

2007 ◽  
Vol 18 (01) ◽  
pp. 91-98 ◽  
Author(s):  
GÖKHAN GÖKOĞLU ◽  
TARIK ÇELİK
Keyword(s):  
Energy State ◽  
Minimum Energy ◽  
Alpha Helix ◽  
Salt Bridge ◽  
Rnase A ◽  
Parallel Tempering ◽  
Implicit Solvent ◽  
C Peptide ◽  
Helix Formation ◽  
Α Helix

We have performed parallel tempering simulations of a 13-residue peptide fragment of ribonuclease-A, c-peptide, in implicit solvent with constant dielectric permittivity. This peptide has a strong tendency to form α-helical conformations in solvent as suggested by circular dichroism (CD) and nuclear magnetic resonance (NMR) experiments. Our results demonstrate that 5th and 8–12 residues are in the α-helical region of the Ramachandran map for global minimum energy state in solvent environment. Effects of salt bridge formation on stability of α-helix structure are discussed.

FABRICATION OF NANOPOROUS CHITOSAN MEMBRANES

NANO ◽  
2010 ◽  
Vol 05 (01) ◽  
pp. 53-60 ◽  
Author(s):  
XIAOLIANG WANG ◽  
XIANG LI ◽  
ELEANOR STRIDE ◽  
MOHAN EDIRISINGHE
Keyword(s):  
Low Frequency ◽  
Drug Carriers ◽  
Structural Features ◽  
Wound Dressings ◽  
Ftir Analysis ◽  
Tissue Engineering Scaffolds ◽  
Nanoscale Structures ◽  
Low Frequency Ultrasound ◽  
Chitosan Membranes ◽  
The Stability

Naturally derived biopolymers have been widely used for biomedical applications such as drug carriers, wound dressings, and tissue engineering scaffolds. Chitosan is a typical polysaccharide of great interest due to its biocompatibility and film-formability. Chitosan membranes with controllable porous structures also have significant potential in membrane chromatography. Thus, the processing of membranes with porous nanoscale structures is of great importance, but it is also challenging and this has limited the application of these membranes to date. In this study, with the aid of a carefully selected surfactant, polyethyleneglycol stearate-40, chitosan membranes with a well controlled nanoscale structure were successfully prepared. Additional control over the membrane structure was obtained by exposing the suspension to high intensity, low frequency ultrasound. It was found that the concentration of chitosan/surfactant ratio and the ultrasound exposure conditions affect the structural features of the membranes. The stability of nanopores in the membrane was improved by intensive ultrasonication. Furthermore, the stability of the blended suspensions and the intermolecular interactions between chitosan and the surfactant were investigated using scanning electron microscope and Fourier transform infrared spectroscopy (FTIR) analysis, respectively. Hydrogen bonds and possible reaction sites for molecular interactions in the two polymers were also confirmed by FTIR analysis.

scholarly journals Novel phthalonitrile derivatives as potential compounds for extraction and complexation of metal cations

2016 ◽  
Vol 11 (10) ◽  
pp. 3870-3874
Author(s):  
Lassaad Baklouti
Keyword(s):  
Heavy Metal ◽  
Transition Metal ◽  
Metal Cations ◽  
Uv Spectra ◽  
Transition Metal Cations ◽  
Binding Properties ◽  
Heavy Metal Cations ◽  
Uv Visible ◽  
The Stability ◽  
Ligand Species

The synthesis and the binding properties of novel phthalonitrile derivatives 1-3 towards metal cations have been described in this paper. The complexation and extraction of some transition and heavy metal cations have been followed by UV-visible spectrophotometry absorption in methanol. The conductivity studies have been used in order to confirm complex’s stoichiometries. The treatment of UV spectra by digital program showed the formation of ML (with ML2 in some cases) (M=metal, L=ligand) species. Beyond the discussion of the stability profiles of complexes particular attention is paid to the selectivity towards Cu2+ in the 1st sequence of transition metal cations and towards Hg2+ in the sequence of heavy metal cations.

THE INFLUENCES OF TEMPERATURE AND CHAIN–CHAIN INTERACTION ON FEATURES OF SOLITONS EXCITED IN α-HELIX PROTEIN MOLECULES WITH THREE CHANNELS

2009 ◽  
Vol 23 (10) ◽  
pp. 2303-2322 ◽  
Author(s):  
XIAO-FENG PANG ◽  
MEI-JIE LIU
Keyword(s):  
Energy Transport ◽  
Initial Conditions ◽  
Dynamic Features ◽  
New Model ◽  
Protein Molecules ◽  
Long Time ◽  
The Stability ◽  
Α Helix ◽  
Increasing Temperature ◽  
Chain Interaction

The dynamic features of soliton transporting the bio-energy in the α-helix protein molecules with three channels under influences of temperature of systems and chain–chain interaction among these channels have been numerically studied by using the dynamic equations in a new model and the fourth-order Runge–Kutta method. This result obtained shows that the chain–chain interaction depresses the stability of the soliton due to the dispersed effect, but the stability of the soliton in the case of simultaneous motivation of three channels by an initial conditions is better than that in another initial condition. We also find from this investigation that the new soliton can transport steadily over 1000 amino acid residues in the cases of motion of long time of 120 ps, and retain their shapes and energies to travel towards the protein molecules after mutual collision of the solitons at the biological temperatures of 300 K. Therefore the soliton is very robust against the thermal perturbation of the α-helix protein molecules at 300 K. From the investigation of changes of features of the soliton with increasing temperature, we find that the amplitudes and velocities of the solitons decrease with increasing temperature of proteins, but the soliton disperses in the cases of higher temperature of 325 K and larger structure disorders. Thus we find that the critical temperature of the soliton occurring in the α-helix protein molecules is about 320 K. Therefore we can conclude that the soliton in the new model can play an important role in the bio-energy transport in the α-helix protein molecules with three channels at biological temperature, and the new model is possibly a candidate for the mechanism of this transport.

scholarly journals On the Stability of 3C-SiC Single Crystals at High Temperatures

2012 ◽  
Vol 717-720 ◽  
pp. 493-496
Author(s):  
Deborah Dompoint ◽  
Irina G. Galben-Sandulache ◽  
Alexandre Boulle ◽  
Didier Chaussende ◽  
Dominique Eyidi ◽  
...  
Keyword(s):  
Single Crystals ◽  
Volume Fraction ◽  
Diffuse Intensity ◽  
X Ray ◽  
Partial Dislocations ◽  
X Ray Scattering ◽  
Transmission Electron ◽  
The Stability ◽  
Kinetics Of ◽  
Evolution With Time

The 3C-6H polytypic transition in 3C-SiC single crystals is studied by means of diffuse X-ray scattering (DXS) coupled with transmission electron microscopy (TEM). TEM reveals that the partially transformed SiC crystals contain regions of significantly transformed SiC (characterized by a high density of stacking faults) co-existing with regions of pure 3C-SiC. The simulation of the diffuse intensity allows to determine both the volume fraction of transformed material and the transformation level within these regions. It is further shown that the evolution with time and temperature of the transition implies the multiplication and glide of partial dislocations, the kinetics of which are quantified by means of DXS.

A Variable Viscosity Approach for the Analysis of Steady State and Dynamic Characteristics of Two-Lobe Journal Bearing With TiO2 Based Nanolubricant

2017 ◽  
Author(s):  
Ashutosh Kumar ◽  
Sashindra Kumar Kakoty
Keyword(s):  
Steady State ◽  
Dynamic Characteristics ◽  
Journal Bearing ◽  
Volume Fraction ◽  
Variable Viscosity ◽  
Flow Coefficient ◽  
Viscosity Model ◽  
Nano Lubricant ◽  
The Stability ◽  
Stiffness And Damping

Steady-state and dynamic characteristics of two-lobe journal bearing, operating on TiO2 based Nano-lubricant has been obtained. The effective viscosity is obtained by using Krieger-Dougherty viscosity model for a given volume fraction of nanoparticle in the base fluid. Various bearing performance characteristics are then obtained by solving modified Reynolds equation for variable viscosity model and couple stress model. The stiffness and damping coefficients are also determined for various values of the volume fraction of the nanoparticle in the nanofluid. Results reveal that load carrying capacity and flow coefficient increase whereas friction variable decreases without affecting the stability condition of two-lobe journal bearing operating on TiO2 based nanolubricant. On the other hand attitude angle and dynamic coefficients remains constant for all the values of volume fraction of nanoparticle.

DEVELOPMENT OF A METHOD FOR QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN TRIPLEUROSPERMUM INODORUM’S FLOWERS

2021 ◽  
pp. 157-166
Author(s):  
Blinova O.L. ◽  
Gileva A.A. ◽  
Hlebnikov A.V. ◽  
Belonogova V.D. ◽  
Turyshev A.Y.
Keyword(s):  
Quantitative Determination ◽  
Aluminum Chloride ◽  
Chloride Solution ◽  
Uv Spectra ◽  
Raw Material ◽  
Spectral Studies ◽  
Scientific Medicine ◽  
Differential Spectrophotometry ◽  
The Stability

Chamomilla recutita is used in scientific medicine. Tripleurospermum inodorum (L.) Sch. Bip. is widely spread among possible impurities of Chamomilla recutita (L.) Rauschert. This plant is perspective for establishment into scientific medicine but it can’t change Chamomilla recutita (L.) Rauschert. Purpose of research: development of a method for the quantitative determination of the amount of flavonoids in terms of rutin in Tripleurospermum inodorum’s flowers using differential spectrophotometry. Materials and methods. The samples prepared in different parts of Russia were used as objects of research. (2017 – 2020). Spectral studies were carried out in the wavelength range of 350-430 nm with a step of 1 nm using a spectrophotometer SF-2000. Results. To determine the analytical wavelength, the UV spectra of alcohol extraction of Tripleurospermum inodorum’s flowers were studied. Maximum of absorption was noticed at wavelength 370 nm. The differential spectrum of the same extraction with an aluminum chloride solution of 2% in 96% alcohol has a maximum at a length of 410±2 nm, which coincides with the maximum of the Standard Sample (SS) of rutin. The largest number of flavonoids is extracted by 70% alcohol. The maximum optical density and the highest output of the number of flavonoids from the raw material is observed at a degree of grinding of 2 mm with a single extraction for 60 minutes. In the conditions of complexation, the optimal ratio of the volume of the test solution and aluminum chloride with a solution of 2% in 96% alcohol is the ratio of 1:1. The stability of the complex with an aluminum chloride solution of 2% in 96% alcohol is observed in 40 minutes after the start of the reaction and retains it for 30 minutes. Findings. The method is developed and the parameters of the quantitative determination of the amount of flavonoids in Tripleurospermum inodorum flores are determined in terms of rutin using differential spectrophotometry.

Reversible Electrochemical Triggering and Optical Interrogation of Polylysine α-helix Formation

2021 ◽  
pp. 108007
Author(s):  
Eloise Masquelier ◽  
Sheng-Ping Liang ◽  
Lior Sepunaru ◽  
Daniel E. Morse ◽  
Michael J. Gordon
Keyword(s):  
Helix Formation ◽  
Α Helix
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