Antifungal Antibiotic from the Mushroom Agrocybe aegerita (BRIG.) SING.

1992 ◽  
Vol 57 (3) ◽  
pp. 590-603 ◽  
Author(s):  
Karel Stránský ◽  
Marta Semerdžieva ◽  
Miroslav Otmar ◽  
Želimír Procházka ◽  
Miloš Buděšínský ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance ◽  
Preparative Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Agrocybe Aegerita ◽  
Antifungal Antibiotic ◽  
Antibiotic Compounds ◽  
Structural Formulae ◽  
Layer Chromatography

An extract from a submersed culture of the mushroom Agrocybe aegerita (BRIG.) SING., containing antifungal antibiotic compounds was chromatographed on a silica gel column. Compounds from fractions which displayed the highest biological activity were concentrated and isolated by means of preparative thin-layer chromatography and preparative high-performance liquid chromatography, and were further characterized by means of gas chromatography, mass spectrometry and nuclear magnetic resonance. They are sesquiterpenic diols predominantly with an illudine skeleton. Structural formulae are proposed for some of them.

scholarly journals N-[4-(1-Methyl-1H-imidazol-2-yl)-2,4′-bipyridin-2′-yl]benzene-1,4-diamine

Molbank ◽  
10.3390/m1030 ◽  
2018 ◽  
Vol 2018 (4) ◽  
pp. M1030 ◽  
Author(s):  
Dhafer Zinad ◽  
Dunya AL-Duhaidahaw ◽  
Ahmed Al-Amiery ◽  
Abdul Kadhum
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance ◽  
Nucleophilic Aromatic Substitution ◽  
Gas Chromatography Mass Spectrometry ◽  
Aromatic Substitution ◽  
Target Compound ◽  
Chromatography Mass Spectrometry ◽  
Snar Reaction ◽  
Layer Chromatography

N-[4-(1-Methyl-1H-imidazol-2-yl)-2,4′-bipyridin-2′-yl]benzene-1,4-diamine was synthesized with a good yield by the reaction of 2′-chloro-4-(1-methyl-1H-imidazol-2-yl)-2,4′-bipyridine with 4-phenylenediamine. The functionalization of the pyridine was accomplished by a nucleophilic aromatic substitution (SNAr) reaction that afforded the target compound. The synthesized compound was characterized by chemical analysis, which includes nuclear magnetic resonance (NMR) (1H-NMR and 13C-NMR), Thin Layer Chromatography-Mass Spectrometry (TLC-MS), high- performance liquid chromatography (HPLC), Gas Chromatography-Mass Spectrometry (GC-MS), and elemental analysis.

Preparation, isolation, and characterization of permethylated galactosides and fucosides

1999 ◽  
Vol 77 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Daniel D Asres ◽  
Hélène Perreault
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Characterization Methods ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Layer Chromatography ◽  
Structural Aspects

Sugar analysis involving permethylation followed by methanolysis can lead to significant results, given that permethylated monosaccharide standards are available for comparison of spectral and chromatographic data. Such standards are not readily commercially available, especially the furanoside forms. This note describes the isolation and characterization of permethylated pyranoside and furanoside species of D(+)-galactose and L(-)-fucose. Separation of the isomers was optimized using a combination of column chromatography and continuous elution thin-layer chromatography (TLC). TLC, gas chromatography - mass spectrometry, and 1H nuclear magnetic resonance (NMR) spectroscopy were used as the characterization methods. The isolation of furanosides is emphasized, since no specific NMR data have been reported on those to date, while several reports have already discussed the structural aspects of pyranosides.Key words: permethylation, monosaccharide, GC-MS, TLC, NMR.

Separation and Identification of Phencyclidine and Some of Its Analogs

1983 ◽  
Vol 66 (5) ◽  
pp. 1186-1195
Author(s):  
K Gurudath Rao ◽  
Shiv K Soni
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Mass Spectra ◽  
Major Ions ◽  
Gas Chromatography Mass Spectrometry ◽  
Structural Variations ◽  
Layer Chromatography

Abstract l-(l-Phenylcyclohexyl)piperidine (PCP) and 9 of its analogs have been separated and identified by using thin layer chromatography, high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and infrared spectroscopy (IR). Spectral and chromatographic characteristics are discussed in terms of the structural variations observed in these compounds. Some compounds decomposed on the GC column; the HPLC procedure is a helpful substitute. A tentative scheme is proposed for the fragmentation of the major ions observed in the mass spectra. The effect of substitution on the IR spectra of the analogs was investigated.

Tetrodotoxin in Gastropods (Snails) Implicated in Food Poisoning in Northern Taiwan

2002 ◽  
Vol 65 (8) ◽  
pp. 1341-1344 ◽  
Author(s):  
DENG-FWU HWANG ◽  
YU-CHIUNG SHIU ◽  
PAI-AN HWANG ◽  
YA-HUI LU
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Food Poisoning ◽  
Gas Chromatography Mass Spectrometry ◽  
Cellulose Acetate Membrane ◽  
Layer Chromatography ◽  
Northern Taiwan

The toxin in the gastropods (snails) Zeuxis sufflatus and Niotha clathrata implicated in a food poisoning incident in northern Taiwan in April 2001 was studied. The symptoms exhibited by four victims were general paresthesia, paralysis of the phalanges and the extremities, paralysis, coma, vomiting, and aphasia. The remaining gastropods were assayed for toxicity in the form of tetrodotoxin (TTX). The ranges of specimen toxicity were 345 to 1,640 mouse units (MU) for Z. sufflatus and 190 to 643 MU for N. clathrata. The toxicities of the digestive gland and for other parts of the gastropod were 1,120 ± 477 MU and 497 ± 238 MU, respectively, for Z. sufflatus and 683 ± 113 MU and 289 ± 169 MU, respectively, for N. clathrata. The toxin from the methanolic extract of the gastropods was partially purified by ultrafiltration and Bio-Gel P-2 column chromatography. Cellulose acetate membrane electrophoresis, thin-layer chromatography, high-performance liquid chromatography, and gas chromatography–mass spectrometry analyses demonstrated that the toxin consisted of TTX. It was concluded that the causative agent of the food poisoning in question was TTX.

scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY, HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHANOLIC SEED EXTRACT OF FICUS BENGHALENSIS LINN

2019 ◽  
Vol 12 (1) ◽  
pp. 205
Author(s):  
Vimala G ◽  
Gricilda Shoba F
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Antimicrobial Agent ◽  
Thin Layer ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Ficus Benghalensis ◽  
Layer Chromatography

Objective: In the traditional medicine, Ficus benghalensis is used for the treatment of ulcer, diabetes, inflammation, liver disorders, urinary disorders, vomiting, vaginal complains, and fever and as a bitter tonic. However, despite its traditional usage as an antimicrobial agent, there is no information regarding its effectiveness in infections caused by pathogenic microbes. Hence, we evaluated the ethanolic extract of the seeds of F. benghalensis for its antimicrobial activity.Methods: The antimicrobial activity of the extract at five different concentrations was tested against few common human pathogenic microorganisms by agar disc diffusion assay. Streptomycin (10 μg/ml) and Amphotericin B (10 μg/ml) were used as standards for antibacterial and antifungal studies, respectively. Few phenolic compounds were identified by standard high-performance thin-layer chromatography (HPTLC) and gas chromatography-mass spectrometry (GC-MS) techniques.Results: The zone of inhibition was extremely great for Pseudomonas aeruginosa (19 mm), Enterococcus faecalis (18 mm), and Aspergillus niger (13 mm). Moreover, through HPTLC analysis, few phenolic compounds such as quercetin (Rf value - 0.54), gallic acid (Rf value - 0.45), and tannic acid (Rf value - 0.45) were identified. A total of seven compounds were identified in the extract using GC-MS analysis.Conclusions: The results, therefore, clearly indicate that the crude extract from F. benghalensis seeds could be used as a potential source of natural antimicrobial agent due to the presence of the phytoconstituent quercetin, gallic acid, and tannic acid in abundance along with other active compounds and support the traditional use of the plant in the treatment of infections.

scholarly journals Flavonoids From the Flowers and Leaves of the Himalayan Megacodon stylophorus (Gentianaceae)

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199226
Author(s):  
Tsukasa Iwashina ◽  
Rinchen Yangzom ◽  
Hari Prasad Devkota ◽  
Takayuki Mizuno
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
The Other ◽  
Liquid Chromatography Mass Spectrometry ◽  
The Family ◽  
First Time ◽  
Layer Chromatography

Five flavonol O-glycosides and 4 C-glycosylflavones were isolated from the flowers of the Himalayan Megacodon stylophorus (Gentianaceae). They were characterized as quercetin 3- O-glucoside (1), quercetin 3- O-rutinoside (2), kaempferol 3- O-glucoside (3), isorhamnetin 3- O-glucoside (4) and kaempferol 3,7-di- O-glucoside (5) (flavonols), and isovitexin (6), isoorientin (7), isovitexin X″- O-arabinoside (8) and isovitexin 4′- O-glucoside (9) ( C-glycosylflavones) by ultraviolet, liquid chromatography-mass spectrometry, acid hydrolysis, nuclear magnetic resonance, and/or high-performance liquid chromatography and thin-layer chromatography comparisons with authentic samples. On the other hand, 5 C-glycosylflavones were isolated from the leaves and identified as 6, 7, 9, vitexin (10), and orientin (11). Although many C-glycosylflavones and xanthones have been reported from Gentianaceae species, flavonols are minor occurrence in the family. Flavonoids were reported from the Megacodon species for the first time.

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