A spectrophotometric assay of Indulin C - A potential new substrate for determination of lignin-degrading abilities of microorganisms

1990 ◽  
Vol 55 (7) ◽  
pp. 1866-1872
Author(s):  
Jiří Gabriel ◽  
Ladislav Homolka ◽  
Roman Krejčí
Keyword(s):  
Spectrophotometric Study ◽  
Spectrophotometric Assay ◽  
Uv Spectrophotometry ◽  
Theoretical Studies ◽  
Model Compounds ◽  
Spectral Behaviour ◽  
Lignin Model ◽  
Electronic Spectral Behaviour ◽  
Derivative Uv Spectrophotometry

A basic spectrophotometric study of a commercially produced sodium salt of Kraft lignin (Indulin C) was performed. The method of quantitative determination of Indulin C based on its absorbance at 276 nm has been found to be applicable. The spectra were also studied by means of derivative UV-spectrophotometry, which is recommended for theoretical studies of electronic spectral behaviour of lignins and lignin model compounds. Linear dependences were found between Indulin C concentration and the second derivative of spectrum at 250 nm and 276 nm. Both methods were used for determining the lignin degrading ability of the basidiomycete Phanerochaete chrysosporium and their suitability is discussed.

scholarly journals Determination of Fleroxacin in Pure and Tablet Forms by Liquid Chromatography and Derivative UV-Spectrophotometry

2003 ◽  
Vol 86 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Dorota Kowalczuk ◽  
Hanna Hopkała
Keyword(s):  
Mobile Phase ◽  
Internal Standard ◽  
Spectrophotometric Assay ◽  
Uv Spectrophotometry ◽  
Aminobenzoic Acid ◽  
Tetrabutylammonium Hydroxide ◽  
Relative Standard ◽  
Liquid Chromatographic ◽  
Derivative Uv Spectrophotometry

Abstract Derivative UV-spectrophotometric and liquid chromatographic (LC) methods for fleroxacin determination were validated. In the spectrophotometric assay, first-, second-, third-, and fourth-order measurements were applied with the use of peak–zero and peak–peak techniques. The linear correlation between amplitude of the peak and concentration of the examined drug ranged from 2.0 to 12.0 μg/mL. An isocratic LC analysis was performed on a Purospher ODS column with an acidic mobile phase containing tetrabutylammonium hydroxide. Measurements were made at a wavelength of 285 nm with 4-aminobenzoic acid (PABA) as internal standard. The calibration curve was linear (r = 0.9999) in the studied range of concentration (1.0–10.0 μg/mL). The accuracy (mean recovery, about 100%), precision (relative standard deviation <1%), selectivity, and sensitivity of the elaborated methods were satisfactory.

Determination of chemical shifts in 6-condensed syringylic lignin model compounds

Holzforschung ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Lucas Lagerquist ◽  
Jani Rahkila ◽  
Patrik Eklund
Keyword(s):  
Chemical Shift ◽  
Methoxy Group ◽  
Chemical Shifts ◽  
Model Compounds ◽  
Correlation Peak ◽  
Lignin Model Compounds ◽  
Benzylic Alcohols ◽  
Lignin Model ◽  
C Nmr

Abstract A small library of 6-substituted syringyl model compounds with aliphatic, carboxylic, phenylic, benzylic alcohols and brominated substituents were prepared. The influence of the substituents on the chemical shifts of the compounds was analyzed. All of model compounds showed a characteristic increase in the 13C NMR chemical shift of the methoxy group vicinal to the substitution. This 13C NMR peak and its corresponding correlation peak in HSQC could potentially be used to identify 6-condensation in syringylic lignin samples.

Determination of fleroxacin in human serum and in dosage forms by derivative UV spectrophotometry

1998 ◽  
Vol 18 (1-2) ◽  
pp. 145-150 ◽  
Author(s):  
Milena Jelikic-Stankov ◽  
Jadranka Odovic ◽  
Dejan Stankov ◽  
Predrag Djurdjevic
Keyword(s):  
Human Serum ◽  
Dosage Forms ◽  
Uv Spectrophotometry ◽  
Derivative Uv Spectrophotometry
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