3'-O-Formyl-(N-acyl)-2'-deoxyribonucleosides as building units in the synthesis of oligodeoxyribonucleotides

1982 ◽  
Vol 47 (8) ◽  
pp. 2157-2169 ◽  
Author(s):  
Jiří Smrt
Keyword(s):  
Acetic Acid ◽  
Acetic Anhydride ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Subsequent Treatment ◽  
Aqueous Acetic Acid ◽  
Formyl Group ◽  
Layer Chromatography ◽  
Methanolic Ammonia

5'-O-Dimethoxytrityl-(N-acyl)-2'-deoxyribonucleosides afford 3'-O-formyl-(N-acyl)-2'-deoxyribonucleosides Ia-Id by the action of formic acetic anhydride followed by the action of 80% aqueous acetic acid. The formyl group is removed from Ia-Id by treatment with 1 mol l-1 triethylamine 3'-O-Formyl-2'-deoxythymidine (Ia) gives 3'-O-dimethoxytrityl-2'-deoxythymidine (V) by subsequent treatment with acetic anhydride, triethylamine, dimethoxytrityl chloride and methanolic ammonia. The use of compounds I for the synthesis of d-GGAGG (XIX) and d-T16 (XXXII) is described. Systems for thin-layer chromatography of 5'-O-dimethyltrityl-oligodeoxyribonucleotides on silica gel are described.

Detection of Cashew Nut Shells in Coffee, Tea, and Chicory

1974 ◽  
Vol 57 (3) ◽  
pp. 761-762
Author(s):  
Pratima Sengupta ◽  
Asit R Sen ◽  
Nomita Ghoshdastidar ◽  
Bidhan R Roy
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Ether Extract ◽  
Cashew Nut ◽  
The Common ◽  
Cashew Nut Shell ◽  
Layer Chromatography ◽  
Ground Coffee

Abstract Thin layer chromatography has been investigated for the identification of cashew nut shell, one of the common adulterants in ground coffee, tea, and chicory. The ether extract of the sample is applied to silica gel C plates and developed with benzene-dioxane-acetic acid (90+25+4) . Cashew nut shell shows 3 distinctive spots (Rf 0.7, 0.54, and 0.34) with diazotized benzidine which are totally absent in tea, coffee, and chicory. The spots have been identified as anacardic acid, cardol, and anacardol, respectively.

Identifikasi Senyawa Jamu Pegal Linu yang Beredar di Kabupaten Bantul dengan Metode Kromatografi Lapis Tipis

2019 ◽  
Vol 14 (2) ◽  
pp. 80
Author(s):  
Furijika Fitriana Mosy ◽  
Kuswandani Kuswandani
Keyword(s):  
Acetic Acid ◽  
Herbal Medicine ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Random Sampling ◽  
Uv Light ◽  
Physicochemical Method ◽  
Layer Chromatography

Background: Traditional herbal is not permitted to contain chemicals drug. It is possible allowed such as paracetamol and phenylbutazone.Objective: This study is aimed to identify the compounds of paracetamol and phenylbutazone in the traditional herbal of Jamu Pegal Linu (Herbal Medicine).Methods: This type of research was descriptive and the sample was determined by random sampling. The method used Thin Layer Chromatography (TLC) which is a physicochemical method. There were eight samples were extracted by the soxhletation method until a thick extract was obtained to be spotted in the quite phase of TLC silica gel F254. The motion phase used for the analysis of paracetamol was chloroform: acetone: toluene (65:25:10) and the motion phase for phenylbutazone analysis was benzene: chloroform: 96% acetic acid (50:40:10). Spots detection was done by observing under UV light of 254 nm and the spots that appeared were calculated for Rf value and compared with the standard Rf value of paracetamol and phenylbutazone.Results: The results obtained in this study were positive E samples containing paracetamol and phenylbutazone with Rf value of sample 0.57 and a standard Rf of paracetamol 0.57 and an Rf value of sample 0.82 and a standard Rf of phenylbutazone 0.86. The positive G sample contained paracetamol with a sample Rf value of 0.61 and a standard Rf value of paracetamol 0.68.Conclusion: From the eight samples of ‘Jamu Pegal Linu’, two of them were positive containing chemical drugs paracetamol and phenylbutazone.Keywords: Jamu Pegal Linu (Herbal Medicine), Paracetamol, Phenylbutazone, TLC

scholarly journals Determination of Oxycodone Hydrochloride in Oral Solutions by High-Performance Thin-Layer Chromatography/Densitometry

2000 ◽  
Vol 83 (6) ◽  
pp. 1497-1501
Author(s):  
Hannele E M Salomies ◽  
Piia K Salo
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Mobile Phase ◽  
High Performance ◽  
Acetic Acid Water ◽  
Oxycodone Hydrochloride ◽  
Layer Chromatography

Abstract A thin-layer chromatography/densitometry method was developed and validated for the determination of oxycodone hydrochloride in oral solutions by using silica gel plates. A horizontal technique was used for development of the plates. The optimum composition for the mobile phase, which provided a suitable Rf value of 0.6 for oxycodone, was propanol–acetic acid–water–25% ammonia–methanol (20 + 1 + 1 + 3 + 10). Detection was at 234 nm. Oxycodone hydrochloride was stable on the sorbent and was precisely and accurately measured in the range of 0.3–1.5 μg/band.

scholarly journals A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner

2021 ◽  
Author(s):  
Barbara Anders ◽  
Sabrina Doll ◽  
Bernd Spangenberg
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Detection System ◽  
Flatbed Scanner ◽  
Butyl Ether ◽  
Quantification Method ◽  
Direct Measurements ◽  
Layer Chromatography

AbstractWe present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The RF value of triclosan is hRF = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

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