Degradation of the peach gum polysaccharide by the mixture of extracellular glycanohydrolases of aspergillus flavus

1980 ◽  
Vol 45 (8) ◽  
pp. 2260-2265 ◽  
Author(s):  
Alžbeta Kardošová ◽  
Jozef Rosík ◽  
Jozef Kubala
Keyword(s):  
Acid Hydrolysis ◽  
Aspergillus Flavus ◽  
Side Chains ◽  
Lower Content ◽  
Partial Acid Hydrolysis ◽  
Glycosidic Bonds ◽  
Peach Gum

The polysaccharide of peach gum, prepared by a partial acid hydrolysis, was degraded by a mixture of extracellular glacanohydrolases of Aspergillus flavus. This way obtained polysaccharide differed from the original one especially by a higher content of D-mannose and a lower content of D-galactose bound by (1 3) glycosidic bonds. The obtained results are discussed from the standpoint of composition of the main and side chains of the native polysaccharide.

Structure of an acidic polysaccharide elaborated by Bacilluspolymyxa-1231-ATCC 842

1990 ◽  
Vol 68 (2) ◽  
pp. 323-328 ◽  
Author(s):  
Salah M. Abdel-Kader ◽  
Mohamady A. Issa ◽  
Mohamed A. El-Shafei
Keyword(s):  
Acid Hydrolysis ◽  
Glucuronic Acid ◽  
Molar Ratio ◽  
Side Chains ◽  
Partial Acid Hydrolysis ◽  
Acidic Polysaccharide ◽  
Backbone Chain ◽  
Branched Structure

An extracellular acidic polysaccharide, elaborated by Bacilluspolymyxa, was composed of D-glucose, D-mannose, D-galactose, and D-glucuronic acid (approximate molar ratio of 4:3:1:1). Methylation and fragmentation analysis by partial acid hydrolysis indicated that the polysaccharide had a complicated, highly branched structure, consisting mainly of β(1 → 3) and (1 → 4) D-glycosidic linkages. The backbone chain containing D-glucose, D-mannose, and D-glucuronic acid residues is attached at the C-4, C-3, and C-4 positions, respectively, one out of every three glucose residues being substituted at the C-2 position to form two kinds of branches at the C-2 and C-4 positions, with side chains of single or a few carbohydrate units that are terminated by D-glucose and (or) D-mannose residues. Keywords: acidic polysaccharide, Bacilluspolymyxa.

CONSTITUTION OF A GLUCOMANNAN FROM THE WOOD OF EASTERN WHITE PINE (PINUS STROBUS L.)

1960 ◽  
Vol 38 (10) ◽  
pp. 1957-1966 ◽  
Author(s):  
M. O. Gyaw ◽  
T. E. Timell
Keyword(s):  
Acid Hydrolysis ◽  
Pinus Strobus ◽  
Partial Hydrolysis ◽  
White Pine ◽  
Eastern White Pine ◽  
Partial Acid Hydrolysis ◽  
Glycosidic Bonds ◽  
Relative Composition ◽  
Linear Macromolecules

Glucomannans have been isolated from the wood of eastern white pine (Pinusstrobus L.) by two different methods, the ratios of mannose to glucose being 3.8:1 and 2.7:1, respectively. The yields, based on the mannose content of the wood, were 70 and 50%. Partial acid hydrolysis gave 4-O-β-D-mannopyranosyl-D-mannose, 4-O-β-D-mannopyranosyl-D-glucose, 4-O-β-D-glucopyranosyl-D-mannose, 4-O-β-D-glucopyranosyl-D-glucose, O-β-D-mannopyranosyl-(1 → 4)-O-β-D-mannopyranosyl-(l → 4)-D-mannose, and O-β-D-mannopyranosyl-(l → 4)-O-β-D-mannopyranosyl-(l → 4)-O-β-D-mannopyranosyl-(l → 4)-D-mannose. The fully methylated polysaccharide on hydrolysis gave a mixture of di-O-methylhexoses, 2,3,6-tri-O-methyl-D-mannose, 2,3,6-tri-O-methyl-D-glucose, and 2,3,4,6-tetra-O-methylhexose (mostly mannose) in a mole ratio of 4:61:21:1. The number-average degrees of polymerization of the methylated and the nitrated glucomannans were 96 and 93. On the basis of this and other evidence it is concluded that the glucomannan contains at least 90 mannose and glucose residues linked together by (1 → 4)-β-glycosidic bonds to linear macromolecules. The nature and relative composition of the oligosaccharides obtained on partial hydrolysis indicate that very few of the glucose residues are contiguous. The glucomannan is compared with similar polysaccharides from the wood of other gymnosperms.

STRUCTURE OF AN ARABINOGALACTAN FROM TAMARACK (LARIX LARICINA)

1961 ◽  
Vol 39 (8) ◽  
pp. 1563-1573 ◽  
Author(s):  
S. Haq ◽  
G. A. Adams
Keyword(s):  
Acid Hydrolysis ◽  
Larix Laricina ◽  
Side Chains ◽  
Main Body ◽  
Partial Acid Hydrolysis ◽  
Hydrolysis Of

Partial acid hydrolysis of an arabinogalactan from tamarack wood yielded:(I) L-arabinose,(II) D-galactose,(III) 3-O-β-L-arabinopyranosyl-L-arabinose,(IV) 6-O-β-D-galactopyranosyl-D-galactose,(V) 3-O-β-D-galactopyranosyl-D-galactose,(VI) O-D-galactopyranosyl(1 → 6)-O-D-galactopyranosyl(1 → 6)-D-galactose,(VII) 3,6-di-O-(galactopyranosyl)-D-galactopyranose,(VIII) O-D-galactopyranosyl(1 → 3)-O-D-galactopyranosyl(1 → 3)-D-galactose,(IX) O-D-galactopyranosyl(1 → 6)-O-D-galactopyranosyl(1 → 3)-D-galactose,(X) O-D-galactopyranosyl(1 → 6)-O-D-galactopyranosyl(1 → 6)-O-D-galactopyranosyl(1 → 3)-D-galactose,(XI) O-D-galactopyranosyl(1 → 6)-O-D-galactopyranosyl(1 → 3)-O-D-galactopyranosyl(1 → 3)-D-galactose.A previously published structure for the tamarack polysaccharide was revised to show that not more than two consecutive 1 → 3 or 1 → 6 linkages respectively were present in the main core of the repeating unit. Some of the 6-O-β-D-galactopyranosyl-D-galactose units occurred as side chains as well as in the main body of the molecule.

Galactomannan from the seed of Indigofera spicata

1969 ◽  
Vol 22 (9) ◽  
pp. 2005 ◽  
Author(s):  
CW Ford
Keyword(s):  
Acid Hydrolysis ◽  
Excellent Agreement ◽  
Side Chains ◽  
Partial Acid Hydrolysis ◽  
End Group ◽  
Indigofera Spicata

A galactomannan, isolated in 3.2% yield from the seeds of Indigofera spicata (Leguminosae), has been shown to consist of D-galactose and D- mannose in the ratio of 1 : 1.82. End group determination by methylation showed 35.5%, in excellent agreement with the result revealed by the periodate method. Partial acid hydrolysis, indicated that the galactose was linked a to the 6-position of the mannose units. The polysaccharide would appear to contain a linear backbone of (1→4) β-D-manno-pyranose units with side-chains of α-D-galactopyranose units attached to C6 of approximately five out of every nine D-mannopyranose units.

Acid Hydrolysis of Glycosidic Bonds in Polysaccharides: Modelling and Stochastic Simulation

1993 ◽  
pp. 1583-1597 ◽  
Author(s):  
José-Henrique Q. Pinto ◽  
Zin-Eddine Dadach ◽  
Alain Lemoyne ◽  
Serge Kaliaguine
Keyword(s):  
Acid Hydrolysis ◽  
Stochastic Simulation ◽  
Glycosidic Bonds ◽  
Hydrolysis Of

Phosphopeptides Obtained by Partial Acid Hydrolysis of α-Casein2

1957 ◽  
Vol 79 (10) ◽  
pp. 2559-2565 ◽  
Author(s):  
N. J. Hipp ◽  
M. L. Groves ◽  
T. L. McMeekin
Keyword(s):  
Acid Hydrolysis ◽  
Partial Acid Hydrolysis ◽  
Hydrolysis Of

scholarly journals Preparation of a Novel Glucuronomannan from Auricularia Auricala and its Immunological Activity

2012 ◽  
Vol 7 (11) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Zonghong Li ◽  
Dan Jiang ◽  
Hongtao Bi ◽  
Dazheng Liu ◽  
Sungju Jang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Acid Hydrolysis ◽  
Lymphocyte Proliferation ◽  
B Lymphocyte ◽  
Glucuronic Acid ◽  
Hot Water ◽  
Immunological Activity ◽  
Partial Acid Hydrolysis ◽  
Ft Ir

A glucuronomannan (AA-4-H, Mw around 4 KDa) was prepared from the fruit bodies of Auricularia auricala by extraction with hot water, deproteination by Sevag reagent, stepwise precipitation with ethanol and partial acid hydrolysis. Monosaccharides analysis revealed that AA-4-H consisted of 91% mannose (Man) and 9% glucuronic acid (GlcA). FT-IR, NMR and methylation analyses indicated that AA-4-H is a branched glucuronomannan. Its main chains are composed of 1, 3-linked α-Man p, side chains are single α-Man p or α-GlcA residues attached to the O-2 and O-6 of Man residues of the main chains. Bioassay indicated that AA-4-H remarkably enhanced B lymphocyte proliferation and increased the production of nitric oxide of macrophages in vitro. Thus, glucuronomannan AA-4-H could be explored as a potential immunostimulation agent.

Structural studies on cross-linked peptides containing lysinonorleucine from elastin of porcine aorta

1977 ◽  
Vol 55 (3) ◽  
pp. 244-248
Author(s):  
Maonique Davril ◽  
Kia-Ki Han
Keyword(s):  
Acid Hydrolysis ◽  
Structural Studies ◽  
Cross Link ◽  
Structural Investigations ◽  
Partial Acid Hydrolysis

Three lysinonorleucine peptides were isolated from mature porcine aorta elastin after enzymic digestions and partial acid hydrolysis. The structural investigations which were performed suggest that different regions containing lysinonorleucine may exist. The role of this cross-link in mature elastin is discussed.

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