Comparison of spectrophotometric methods of determining Fe(III) using a computer-controlled SuperScanR 3 UV-VIS spectrophotometer

1980 ◽  
Vol 45 (6) ◽  
pp. 1793-1804 ◽  
Author(s):  
Dagmar Gotzmannová ◽  
Vlastimil Kubáň
Keyword(s):  
Blood Serum ◽  
Calibration Curve ◽  
Direct Determination ◽  
Spectrophotometric Methods ◽  
Chromazurol S ◽  
Direct Recording ◽  
Basic Parameters ◽  
Computer Controlled

Methods of the direct determination of Fe(III) with Eriochromazurol B, Chromazurol S, Eriochromcyanine R, 4-(2-pyridylazo)resorcinol and 4-(2-thiazolylazo)resorcinol were compared using basic parameters for the spectrophotometric methods. The most useful reagents for determining Fe(III) in blood serum are Chromazurol S and PAR. The possibility of direct recording and treatment of the calibration curve using the SuperScanR 3 spectrophotometer controlled by an HP 9815A desk-top calculator is demonstrated.

Direct Fluorometric Determination of Total Cholesterol in Serum Using Derivatized Cholesterol Oxidase

2000 ◽  
Vol 54 (8) ◽  
pp. 1157-1162 ◽  
Author(s):  
Javier Galbán ◽  
José F. Sierra ◽  
José M. López Sebastian ◽  
Susana de Marcos ◽  
Juan R. Castillo
Keyword(s):  
Blood Serum ◽  
Total Cholesterol ◽  
Cholesterol Oxidase ◽  
Enzymatic Reaction ◽  
Direct Determination ◽  
Analytical Signal ◽  
Cholesterol Concentration ◽  
Serum Samples ◽  
Response Range

In this paper the use of cholesterol oxidase derivatized with a fluorescein derivative is proposed for the direct determination of total cholesterol in blood serum. The method is based on the changes in the fluorescence of the solution during the enzymatic reaction (λexe = 498 nm and λem 519 nm). A mathematical model which relates the analytical signal to the total cholesterol concentration was developed, and the model can also be used to obtain some of the thermodynamic constants of the process. The method has a linear response range up to 70 mg/L of cholesterol, a detection limit of 2.5 mg/L, and the precision was 1.0% (40 mg/L cholesterol, n = 10). The method was applied to total cholesterol determination in blood serum samples. The results were compared to those obtained by a commercial analyzer, and statistically similar results were obtained. The use of derivatized cholesterol oxidase makes it possible to simplify the methodology normally used in this type of determination (the indicator reaction is avoided and the number of reagents reduced), with the added advantage that the analytical signal is independent of the concentrations of O2 and cholesterol oxidase.

Comparison of the reactions of ferric ions with Chromazurol S, Eriochromazurol B and Eriochromcyanine R in the presence of surface active substances and spectrophotometric determination of Fe(III)-ions with Chromazurol S-cetylpyridinium bromide system

1980 ◽  
Vol 45 (10) ◽  
pp. 2656-2669 ◽  
Author(s):  
Vlastimil Kubáň ◽  
Dagmar Gotzmannová
Keyword(s):  
Ferric Ions ◽  
Cetylpyridinium Bromide ◽  
Absorption Coefficients ◽  
Statistical Parameters ◽  
Spectrophotometric Methods ◽  
Chromazurol S ◽  
Surface Active ◽  
Active Substances ◽  
Serum Components

The spectrophotometric methods of determining Fe(III) ions with Chromazurol S, Eriochromazurol B and Eriochromcyanine R in the presence of cationic, anionic and nonionic tensides were compared using the basic optical and statistical parameters. A method was proposed and developed for the determination of Fe(III) ions using Chromazurol S in blood serum and plasma at pH 4.7-5.0 in a medium of 2 . 10-4M cetylpyridinium bromide and 0.1M pyridine buffer and at pH 6.0-6.5 in a medium of 2 . 10-3M cetylpyridinium bromide and 0.1M hexamethylenetetramine buffer with molar absorption coefficients of 1.31 . 105 and 1.40 . 105, respectively. The determination is sufficiently specific even at pathological concentrations of the other serum components.

scholarly journals Properties of naproxen ion-selective electrodes

2008 ◽  
Vol 6 (4) ◽  
pp. 513-519 ◽  
Author(s):  
Joanna Lenik ◽  
Cecylia Wardak ◽  
Barbara Marczewska
Keyword(s):  
Calibration Curve ◽  
Limit Of Detection ◽  
Standard Addition ◽  
Measurement Range ◽  
Ion Selective Electrodes ◽  
Membrane Electrodes ◽  
Basic Parameters ◽  
Curve Method ◽  
Tetraoctylammonium Chloride

AbstractNaproxen membrane electrodes based on different plasticizers and the quaternary ammonium salts (QASs) dimethyldidecylammonium bromide, methyltrioctylammonium chloride, or tetraoctylammonium chloride, were prepared. The following basic parameters were investigated for the optimal electrode: measurement range (10−4 − 10−1 mol L−1), slope of the linear range of the calibration curve (−58.3 mV decade−1), limit of detection (6.0 × 10−5 mol L−1), lifetime (2.5 months), dependence of the electrode potential on pH (5.5 − 9.0), reproducibility of potential (1.2 mV) and selectivity coefficients in relation to selected organic and inorganic anions. The electrode was utilized for determination of naproxen in tablets by the calibration curve method and the standard addition method.

Direct Determination of Glucose in Blood Serum Using Trinder's Reaction

1984 ◽  
Vol 17 (5) ◽  
pp. 385-396 ◽  
Author(s):  
M. J. Medina ◽  
J. Bartroli ◽  
J. Alonso ◽  
M. Blanco ◽  
J. Fuentes
Keyword(s):  
Blood Serum ◽  
Direct Determination

Direct Determination of Potassium in Human Blood Serum by Flow Injection Flame Photometry with Automatic Dilution

1996 ◽  
Vol 29 (10) ◽  
pp. 1719-1727 ◽  
Author(s):  
Ana Cláudia de Paiva ◽  
Graciliano de Oliveira Neto ◽  
Matthieu Tubino ◽  
Nelci F. Hoehr
Keyword(s):  
Blood Serum ◽  
Flow Injection ◽  
Human Blood ◽  
Direct Determination ◽  
Human Blood Serum ◽  
Flame Photometry

scholarly journals Masking is the Effective Alternative to the Separation during Osmium Determination by Means of Azo Dyes in Complex Samples

2010 ◽  
Vol 4 (2) ◽  
pp. 115-124
Author(s):  
Mariana Rydchuk ◽  
◽  
Teodoziya Vrublevska ◽  
Mariya Boyko ◽  
Olha Korkuna ◽  
...  
Keyword(s):  
Direct Determination ◽  
Complex Samples ◽  
Spectrophotometric Methods ◽  
Masking Agents ◽  
Platinum Group ◽  
Monoazo Dyes ◽  
Interfering Ions ◽  
Osmium Determination ◽  
Better Than

The selectivity of the spectrophotometric methods for determination of microgram quantities of osmium (IV) with acidic monoazo dyes Tropaeolin O (TpO) and Tropaeolin OOO-I (TpOOOI) in the presence of concomitant metals, particularly platinum group, heavy and rare earths (RE) has been studied. The tolerance ratios of interfering elements have been established. The selectivity of the interaction of TpOOOI with osmium (IV) relatively to platinum group elements (PGEs) is much better than TpO. It has been established that the influence of interfering ions can be easily eliminated by means of masking agents viz. EDTA, tartrate, citrate, pyrophosphate and fluoride, and thereby the selectivity of the methods of osmium determination with TpO and TpOOOI has been sufficiently improved. The methods have been approved during the analyses of various model solutions containing ruthenium, platinum, palladium, rhodium and iridium and the masking reagents. Also osmium content has been determined in the intermetallic alloy Nd20Os15Sі65. The standard deviations did not exceed typical values for spectrophotometric methods (RSDs < 5 %). Thus the elaborated methods with various masking agents may be used for direct determination of osmium in complex samples without any preliminary separation of osmium in the form of OsO4 as well as without the extraction of matrices elements.

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