Chromatography of anions on silica gel thin layer

1979 ◽  
Vol 44 (5) ◽  
pp. 1406-1412 ◽  
Author(s):  
Jaroslav Beneš
Keyword(s):  
Dielectric Constant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Aqueous Phase ◽  
Aqueous Ammonia ◽  
Phase Ratio ◽  
Ammonium Ions ◽  
Layer Chromatography

The paper gives results of investigation of behaviour of the anions PO43-, MoO42-, TcO4-, RuO42-, TeO32- and (in part) I- during thin-layer chromatography with silica gel adsorbent and mixtures of alcohols with aqueous ammonia as eluent systems. For migrating anions the RF changes due to changes of the organic to aqueous phase ratio, analytical concentration of ammonium ions, the alcohol used in the eluent system, and concentration of the anions in solutions of samples have been determined. Correlation between RF of the anions and dielectric constant of the eluent systems have been followed; relation between the both variables can be expressed by approximative equation RF = k log ε + log q.

Behaviour of iodide, iodate and periodate in TLC with alcohol-aqueous ammonia eluent

1979 ◽  
Vol 44 (4) ◽  
pp. 1034-1039 ◽  
Author(s):  
Jaroslav Beneš
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Aqueous Phase ◽  
Aqueous Ammonia ◽  
Ammonium Ions ◽  
Open Chain ◽  
Alcohol Water ◽  
Layer Chromatography ◽  
Kinetics Of

The behaviour of I-, IO3- and IO4- in silica gel thin layer chromatography and their separation with ammonia-alcohol eluents (saturated open-chain C1 to C5 alcohols being used) have been studied with the help of radioisotopes. Changes in RF values of the studied anions caused by changes of the alcohol, ratio of the organic to aqueous phase in the mixture, and overall analytical concentration of ammonium ions have been determined. Influence of alcohol on kinetics of development of the chromatograms with the alcohol-water mixtures has been determined, too.

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

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