Amino acid sequence of cyanogen bromide fragment CB3(Cys) of human serum albumin

1977 ◽  
Vol 42 (4) ◽  
pp. 1248-1261 ◽  
Author(s):  
B. Meloun ◽  
L. Morávek
Keyword(s):  
Amino Acid ◽  
Human Serum Albumin ◽  
Amino Acid Sequence ◽  
Serum Albumin ◽  
Human Serum ◽  
Cyanogen Bromide ◽  
Cyanogen Bromide Fragment

Amino Acid Sequence ofN-Terminal Peptide of Normal Human Serum Albumin

1971 ◽  
Vol 18 (3) ◽  
pp. 137-144 ◽  
Author(s):  
C.S. Liu ◽  
T. B. Shih ◽  
M.H. Hsin ◽  
R. Q. Blackwell
Keyword(s):  
Amino Acid ◽  
Human Serum Albumin ◽  
Amino Acid Sequence ◽  
Serum Albumin ◽  
Human Serum ◽  
Normal Human Serum ◽  
Normal Human

Steric accessibility of tyrosine residues in human serum albumin

1979 ◽  
Vol 44 (5) ◽  
pp. 1657-1670 ◽  
Author(s):  
Ladislav Morávek ◽  
Mohamed Ali Saber ◽  
Bedřich Meloun
Keyword(s):  
Amino Acid ◽  
Secondary Structure ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Amino Acid Analysis ◽  
Cyanogen Bromide ◽  
Tyrosine Residues ◽  
Steric Accessibility ◽  
The Individual

Human serum albumin was nitrated by an excess of tetranitromethane at pH 8.0. As shown by amino acid analysis, of the 18 tyrosine residues present in albumin about 7-7.5 residues remain unaltered, 9 residues are converted into 3-nitrotyrosine, and 1.2 residue into 3,5-dinitrotyrosine. The nitrated albumin was digested with cyanogen bromide to three fragments which comprise the whole original molecule. The individual fragments were converted into their S-sulfo derivatives and the latter digested with chymotrypsin or stepwise with trypsin and thermolysin. The yellow, nitrotyrosine-containing peptides were isolated from the digest and the positions of nitrated tyrosine residues in albumin thus located. Residues No 30, 148, 150, 161, 334, 341, 401, and 411 were identified as strongly nitrated and residues No 84, 138, 452, and 497 as medium nitrated. Residues No 140, 263, 319, 332, 353, and 367 either react weakly or were not found in nitrated form. Residue No 411 and partly also 161 were converted into 3,5-dinitrotyrosine. The accessibility of the individual tyrosine residues to the nitrating agent is discussed with respect to their positions in disulfide loops and hypothetic parts of the secondary structure of albumin.

scholarly journals Complete amino acid sequence of human serum albumin

FEBS Letters ◽  
1975 ◽  
Vol 58 (1-2) ◽  
pp. 134-137 ◽  
Author(s):  
B. Meloun ◽  
L. Morávek ◽  
V. Kostka
Keyword(s):  
Amino Acid ◽  
Human Serum Albumin ◽  
Amino Acid Sequence ◽  
Serum Albumin ◽  
Human Serum ◽  
Complete Amino Acid Sequence

Amino acid sequence of cyanogen bromide fragment CB3 of hog pepsin

1981 ◽  
Vol 46 (3) ◽  
pp. 655-666
Author(s):  
Ladislav Morávek ◽  
Vladimír Kostka
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyanogen Bromide ◽  
Methionine Residues ◽  
Cyanogen Bromide Fragment

On the basis of the knowlidge of thermolytic, chymotryptic and substilisin peptides the amino acid sequence was determined of cyanogen bromide fragment CB3 representing the region between methionine residues I and II of pepsin: Thr-Gly-Ile-Leu-Gly-Tyr-Asp-Thr-Val-Gln-Val-Gly-Gly-Ile-Ser-Asp-Thr-Asn-Gln-Ile-Phe-Gly-Leu-Ser-Glu-Thr-Glu-Pro-Gly-Ser-Phe-Leu-Tyr-Tyr-Ala-Pro-Phe-Asp-Gly-Ile-Leu-Gly-Leu-Ala-Tyr-Pro-Ser-Ile-Ser-Ala-Ser-Gly-Ala-Thr-Pro-Val-Phe-Asp-Asn-Leu-Trp-Asp-Gln-Gly-Leu-Val-Ser-Gln-Asp-Leu-Phe-Ser-Val-Tyr-Leu-Ser-Ser-Asn-Asp-Asp-Ser-Gly-Ser-Val-Val-Leu-Leu-Gly-Gly-Ile-Asp-Ser-Ser-Tyr-Tyr-Thr-Gly-Ser-Leu-Asn-Trp-Val-Pro-Val-Ser-Val-Glu-Gly-Tyr-Trp-Gln-Ile-Thr-Leu-Asp-Ser-Ile-Thr-Met.

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