Activity of covalently bound trypsin in partly nonaqueous media

1973 ◽  
Vol 38 (7) ◽  
pp. 2011-2015 ◽  
Author(s):  
T. Barth ◽  
K. Jošt ◽  
I. Rychlík
Keyword(s):  
Nonaqueous Media ◽  
Covalently Bound

Oxygen Sensing in Nonaqueous Media Using Porous Glass with Covalently Bound Luminescent Ru(II) Complexes

1998 ◽  
Vol 70 (24) ◽  
pp. 5184-5189 ◽  
Author(s):  
María P. Xavier ◽  
David García-Fresnadillo ◽  
María C. Moreno-Bondi ◽  
Guillermo Orellana
Keyword(s):  
Porous Glass ◽  
Oxygen Sensing ◽  
Nonaqueous Media ◽  
Covalently Bound

Adsorption of Fibrinogen and Fragment D of Fibrinogen onto the Insolubilized αChain of Fibrin

1979 ◽  
Vol 41 (04) ◽  
pp. 687-690
Author(s):  
F R Matthias
Keyword(s):  
Disulfide Bridges ◽  
Soluble Fibrin ◽  
Immobilized Proteins ◽  
Covalently Bound

SummaryAfter thrombin treatment insolubilized fibrinmonomer, which is obtained from insolubilized fibrinogen covalently bound to agarose, adsorbs soluble fibrin and its derivatives from solutions. The immobilized proteins are attached to the agarose by the ‘A’ αchain. After reduction of the disulfide bridges the β and γchains can be removed from the agarose.After thrombin treatment the immobilized αchain adsorbs fibrinogen and fragment D. To some extent the β and γchain do not seem necessary for the adsorption. The amount adsorbed increases, when thrombin treatment of the insolubilized protein follows the reduction process.This may indicate that the fibrinopeptides ‘A’ of the insolubilized αchain are better accessible after the removal of the β and γchains.

INTERFERENCE BY SERUM PROTEINS WITH LH RADIOIMMUNOASSAY USING IMMUNOSORBENT

1973 ◽  
Vol 72 (2) ◽  
pp. 235-242 ◽  
Author(s):  
A. M. Reuter ◽  
J. C. Hendrick ◽  
J. Sulon ◽  
P. Franchimont
Keyword(s):  
Molecular Weight ◽  
Human Serum ◽  
High Molecular Weight ◽  
Serum Proteins ◽  
Void Volume ◽  
High Molecular Weight Proteins ◽  
Serum Fractionation ◽  
Covalently Bound

ABSTRACT The percentage of LH* bound to antibodies that have been covalently bound to cellulose is diminished in the presence of LH-free human serum and sera from various species of animals. Serum fractionation studies on Sephadex G 200 show that the greatest interference comes from the proteins eluted in the void volume i. e. the high molecular weight proteins. Specifically, the gamma M globulins and the α2-macroglobulins appear to play an important role, as demonstrated by tests in which these proteins were neutralized by gamma M and α2-macroglobulin antisera.

COMPARISON BETWEEN COVALENTLY BOUND AND FREE ANTIBODIES, USED FOR RADIOIMMUNOASSAY

1971 ◽  
Vol 68 (3) ◽  
pp. 425-430 ◽  
Author(s):  
J. Arends
Keyword(s):  
Covalently Bound

ABSTRACT Radioimmunoassays using either free or covalently bound antibodies under otherwise identical conditions have been compared. A lowered utilization of antibodies and a loss in sensitivity protential have been observed with the last mentioned method.

Influence of medium on alkaline hydrolysis of succinic acid monomethyl and monopropyl esters

1980 ◽  
Vol 45 (11) ◽  
pp. 2873-2882
Author(s):  
Vladislav Holba ◽  
Ján Benko
Keyword(s):  
Succinic Acid ◽  
Kinetic Parameters ◽  
Alkaline Hydrolysis ◽  
Specific Interactions ◽  
Nonaqueous Media ◽  
The Media ◽  
Kinetics Of ◽  
Hydrolysis Of

The kinetics of alkaline hydrolysis of succinic acid monomethyl and monopropyl esters were studied in mixed aqueous-nonaqueous media at various temperatures and ionic strengths. The results of measurements are discussed in terms of electrostatic and specific interactions between the reactants and other components of the reaction mixture. The kinetic parameters in the media under study are related to the influence of the cosolvent on the solvation sphere of the reactants.

scholarly journals Mechanochemical Release of Non‐Covalently Bound Guests from a Polymer‐Decorated Supramolecular Cage

2021 ◽  
Author(s):  
Robin Küng ◽  
Tobias Pausch ◽  
Dustin Rasch ◽  
Robert Göstl ◽  
Bernd M. Schmidt
Keyword(s):  
Covalently Bound
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