Toxin-binding proteins isolated from yellow mealworm Tenebrio molitor and wax moth Galleria mellonella

2011 ◽  
Vol 76 (2) ◽  
pp. 202-208 ◽  
Author(s):  
N. V. Bulushova ◽  
D. P. Zhuzhikov ◽  
L. I. Lyutikova ◽  
N. E. Kirillova ◽  
I. A. Zalunin ◽  
...  
Keyword(s):  
Binding Proteins ◽  
Galleria Mellonella ◽  
Tenebrio Molitor ◽  
Yellow Mealworm ◽  
Toxin Binding ◽  
Wax Moth

scholarly journals Biodegradation of Polystyrene by Tenebrio molitor, Galleria mellonella, and Zophobas atratus Larvae and Comparison of Their Degradation Effects

Polymers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 3539
Author(s):  
Shan Jiang ◽  
Tingting Su ◽  
Jingjing Zhao ◽  
Zhanyong Wang
Keyword(s):  
Gut Microbiota ◽  
Galleria Mellonella ◽  
Degradation Process ◽  
Tenebrio Molitor ◽  
Community Diversity ◽  
Intestinal Microbiome ◽  
Yellow Mealworm ◽  
Greater Wax Moth ◽  
Zophobas Atratus ◽  
Wax Moth

Plastic waste pollution and its difficult degradation process have aroused widespread concern. Research has demonstrated that the larvae of Tenebrio molitor (yellow mealworm), Galleria mellonella (greater wax moth), and Zophobas atratus (superworm) possess a biodegradation ability for polystyrene (PS) within the gut microbiota of these organisms. In this study, the difference in PS degradation and the changes of the gut microbiota were compared before and after feeding PS. The results showed that superworm had the strongest PS consumption capacity and the highest survival rate during the 30 d experiment period. They all could degrade PS to different degrees. Superworm showed the highest ability to degrade PS into low-molecular-weight substances, while yellow mealworm depolymerized PS strongly by destroying the benzene ring. The changes of the intestinal microbiome caused by feeding PS showed that after ingesting PS, there was a decrease in community diversity in superworm and yellow mealworm, but an increase in greater wax moth. Meanwhile, Enterococcus and Enterobacteriaceae, found in all three species’ larvae upon 20 d of PS feeding, might play an important role in PS degradation. The results will provide more accurate PS degradation comparative data of the three species’ larvae and theoretical guidance for further research on the efficient PS biodegradations.

Biosynthesis and Secretion of Serine Peptidase SerP38 from Tenebrio molitor in the Yeast Komagataella kurtzmanii

2020 ◽  
Vol 36 (6) ◽  
pp. 78-86
Author(s):  
P.I. Akentyev ◽  
I.I. Gubaidullin ◽  
N.I. Zhiganov ◽  
V.F. Tereshchenkova ◽  
E.N. Elpidina ◽  
...  
Keyword(s):  
Culture Medium ◽  
Polypeptide Chain ◽  
Tenebrio Molitor ◽  
Enzyme Secretion ◽  
Trypsin Treatment ◽  
Yellow Mealworm ◽  
Serine Peptidase

A strain of the Komagataella kurtzmanii yeast, a producer of recombinant peptidase SerP38 from the yellow mealworm Tenebrio molitor, has been obtained. The level of the pro-enzyme secretion was 20-50 mg/L. It was shown that, during secretion in yeast, the target His6-tagged protein was produced in two forms. One of them was a monomer that was efficiently purified by Ni-NTA chromatography and then activated with trypsin. Another form accumulated in the culture medium as oligomers prone to aggregation in the presence of Ni2+ ions and was not activated by trypsin treatment. Aggregation is likely the result of incorrect folding of the polypeptide chain. Tenebrio molitor, S1 family serine peptidase, SerP38, yeast, Komagataella kurtzmanii, ion-dependent aggregation

scholarly journals Graded Incorporation of Defatted Yellow Mealworm (Tenebrio molitor) in Rainbow Trout (Oncorhynchus mykiss) Diet Improves Growth Performance and Nutrient Retention

Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 187 ◽  
Author(s):  
Paulo Rema ◽  
Subramanian Saravanan ◽  
Benjamin Armenjon ◽  
Constant Motte ◽  
Jorge Dias
Keyword(s):  
Body Composition ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Growth Performance ◽  
Tenebrio Molitor ◽  
Nutrient Digestibility ◽  
Protein Meal ◽  
Yellow Mealworm ◽  
Juvenile Rainbow Trout ◽  
Rainbow Trout Oncorhynchus Mykiss

Insects are emerging as a sustainable alternative to fishmeal and fish oil in aquafeeds. This study assessed the effect of graded incorporation levels of defatted yellow mealworm (Tenebrio molitor) protein meal on juvenile rainbow trout (Oncorhynchus mykiss) growth performance, body composition, and apparent nutrient digestibility. The trial comprised five dietary treatments: control diet with 25% fishmeal, and four experimental diets with yellow mealworm protein meal at 5%, 7.5%, 15%, or 25%, which corresponded to a fishmeal replacement of 20%, 30%, 60%, or 100%, respectively. After 90 days, the graded incorporation of insect protein meal led to a significant stepwise increase in final body weight, and a significant improvement of specific growth rate, feed conversion ratio, and protein efficiency ratio compared to the control treatment. Regardless of the incorporation level, the insect protein meal had no effects on fish whole-body composition and apparent digestibility coefficients of dry matter, protein, fat, phosphorus, and energy. Protein, phosphorus, and energy retention significantly increased in fish fed the diets with an insect protein meal. In conclusion, the yellow mealworm protein meal could effectively replace 100% of fishmeal in the diet of juvenile rainbow trout with positive effects on its overall zootechnical performance.

scholarly journals Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1900-1920
Author(s):  
Aiste Dijokaite ◽  
Maria Victoria Humbert ◽  
Emma Borkowski ◽  
Roberto M La Ragione ◽  
Myron Christodoulides
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Wax Moth

Upregulation of hemolymph and tissue ferritin by dietary HgCl2in the wax moth Galleria mellonella

2006 ◽  
Vol 36 (4) ◽  
pp. 185-190 ◽  
Author(s):  
Chi-Won CHOI ◽  
Dong-Hwan SEO ◽  
Ji-Eun YUK ◽  
Jong-Bae PARK ◽  
Seock-Yeon HWANG ◽  
...  
Keyword(s):  
Galleria Mellonella ◽  
Wax Moth

Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco

2006 ◽  
Vol 387 (5) ◽  
pp. 549-557 ◽  
Author(s):  
Gregor Langen ◽  
Jafargholi Imani ◽  
Boran Altincicek ◽  
Gernot Kieseritzky ◽  
Karl-Heinz Kogel ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Fungal Pathogens ◽  
Galleria Mellonella ◽  
Ectopic Expression ◽  
Pathogenic Fungi ◽  
Transgenic Expression ◽  
Greater Wax Moth ◽  
Insect Defensin ◽  
Wax Moth

Abstract A cDNA encoding gallerimycin, a novel antifungal peptide from the greater wax moth Galleria mellonella, was isolated from a cDNA library of genes expressed during innate immune response in the caterpillars. Upon ectopic expression of gallerimycin in tobacco, using Agrobacterium tumefaciens as a vector, gallerimycin conferred resistance to the fungal pathogens Erysiphe cichoracearum and Sclerotinia minor. Quantification of gallerimycin mRNA in transgenic tobacco by real-time PCR confirmed transgenic expression under control of the inducible mannopine synthase promoter. Leaf sap and intercellular washing fluid from transgenic tobacco inhibited in vitro germination and growth of the fungal pathogens, demonstrating that gallerimycin is secreted into intercellular spaces. The feasibility of the use of gallerimycin to counteract fungal diseases in crop plants is discussed.

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