Spectral Characteristics of Indocyanine Green upon Its Interaction with Biological Tissues

2005 ◽  
Vol 99 (4) ◽  
pp. 560 ◽  
Author(s):  
V. I. Kochubey
Keyword(s):  
Indocyanine Green ◽  
Spectral Characteristics ◽  
Biological Tissues

scholarly journals The study of the spectral characteristics of biological tissues for optimization of surgical lamp parameters

2019 ◽  
Vol 1400 ◽  
pp. 066024 ◽  
Author(s):  
A V Mamoshin ◽  
E S Seryogina ◽  
E V Potapova ◽  
A I Shepeleva ◽  
V V Shupletsov ◽  
...  
Keyword(s):  
Spectral Characteristics ◽  
Biological Tissues

Terahertz spectral characteristics of biological tissues

2015 ◽  
Author(s):  
Wei Liu ◽  
Cheng-zhen Lu ◽  
Zhao-feng Jiang ◽  
Ping Sun
Keyword(s):  
Spectral Characteristics ◽  
Biological Tissues

A systematic study on fluorescence contrast in near infrared diffuse transmittance imaging with indocyanine green

2019 ◽  
Vol 27 (5) ◽  
pp. 333-344
Author(s):  
María V Waks Serra ◽  
Dirk Grosenick ◽  
Rainer Macdonald ◽  
Juan A Pomarico ◽  
Daniela I Iriarte
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Experimental Studies ◽  
Biological Tissues ◽  
High Ratio ◽  
Infrared Light ◽  
Noninvasive Technique ◽  
Indocyanine Green Fluorescence ◽  
Fluorescence Contrast

Near infrared fluorescence imaging is a sensitive, noninvasive technique for diagnostic applications in biomedical optics. The main purpose of this work is thus to explore how to improve the contrast of images obtained by near infrared light using a fluorescent extrinsic agent. Among different fluorophores, indocyanine green has been mostly studied because it is approved for use in humans. In this work, simulations and experimental studies on phantoms (systems that optically emulate biological tissues) are used to systematically investigate the influence of the increased intrinsic tissue absorption by adding indocyanine green. The experiments reproduce the situation of fluorescence imaging of carcinomas in the human breast, where the natural absorption due to neovascularization is increased by the injection of this fluorophore. Assuming measurements in transmission geometry, the breast is modeled by a homogeneous background medium containing a tumor-like inclusion (or lesion) with two- or threefold increased absorption. Fluorescence contrast is simulated over a broad range of dye concentrations using diffusion theory. Selected concentrations ratios are applied in experimental studies with laser excitation of indocyanine green fluorescence and with a charge-coupled device camera for fluorescence detection. Both simulations and experiments show that the intrinsic absorption of the inclusion strongly reduces the number of detected fluorescence photons and that the fluorescence contrast can be canceled or become even negative. It was found that for typical optical properties and geometrical conditions, in fluorescence imaging of breast cancer, a dye ratio of about 10:1 (lesion:background) is required to turn from negative to positive fluorescence contrast. Since such a high ratio is difficult to attain, raw fluorescence images need to be normalized by the intrinsic lesion absorption (without indocyanine green (ICG)) to enhance the presence of the dye in the lesion.

Temperature-stimulated changes in the spectral characteristics of biological tissues

2020 ◽  
Author(s):  
Irina Yu . Yanina ◽  
Alexander A. Skaptsov ◽  
Julia G. Konyukhova ◽  
Natalia I. Kazadaeva ◽  
Elena A. Sagaidachnaya ◽  
...  
Keyword(s):  
Spectral Characteristics ◽  
Biological Tissues

Visualisation of the distributions of melanin and indocyanine green in biological tissues

2008 ◽  
Vol 38 (3) ◽  
pp. 263-268 ◽  
Author(s):  
E A Genina ◽  
I V Fedosov ◽  
A N Bashkatov ◽  
D A Zimnyakov ◽  
G B Altshuler ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Biological Tissues

scholarly journals Laser-initiated decomposition products of indocyanine green (ICG) and carbon black sensitized biological tissues

1997 ◽  
Author(s):  
John M. Kokosa ◽  
Andrzej Przyjazny ◽  
Kenneth E. Bartels ◽  
Massoud Motamedi ◽  
Donald J. Hayes ◽  
...  
Keyword(s):  
Carbon Black ◽  
Indocyanine Green ◽  
Biological Tissues ◽  
Decomposition Products

scholarly journals Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Regina Mačianskienė ◽  
Mantė Almanaitytė ◽  
Rimantas Treinys ◽  
Antanas Navalinskas ◽  
Rimantas Benetis ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Spectral Characteristics ◽  
Green Fluorescence ◽  
Indocyanine Green Fluorescence

Three-Dimensional Visualization of the T-System of Frog Muscle Using High Voltage Electron Microscopy and a Lanthanum Stain

1977 ◽  
Vol 35 ◽  
pp. 570-571 ◽  
Author(s):  
Lee D. Peachey ◽  
Clara Franzini-Armstrong
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Three Dimensional ◽  
Biological Tissues ◽  
High Voltage Electron Microscopy ◽  
Transverse Tubular System ◽  
Peroxidase Reaction ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
T System

The effective study of biological tissues in thick slices of embedded material by high voltage electron microscopy (HVEM) requires highly selective staining of those structures to be visualized so that they are not hidden or obscured by other structures in the image. A tilt pair of micrographs with subsequent stereoscopic viewing can be an important aid in three-dimensional visualization of these images, once an appropriate stain has been found. The peroxidase reaction has been used for this purpose in visualizing the T-system (transverse tubular system) of frog skeletal muscle by HVEM (1). We have found infiltration with lanthanum hydroxide to be particularly useful for three-dimensional visualization of certain aspects of the structure of the T- system in skeletal muscles of the frog. Specifically, lanthanum more completely fills the lumen of the tubules and is denser than the peroxidase reaction product.

On the High Voltage Electron Microscopy (1 MeV) of Biological Thick Sections

1972 ◽  
Vol 30 ◽  
pp. 464-465
Author(s):  
William H. Massover
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Present Report ◽  
Biological Tissues ◽  
Specimen Preparation ◽  
Technical Problems ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron

Stereoscopic examination of thick sections of fixed and embedded biological tissues by high voltage electron microscopy has been shown to allow direct visualization of three-dimensional fine structure. The present report will consider the occurrence of some new technical problems in specimen preparation and Image interpretation that are not common during lower voltage studies of thin sections.Thick Sectioning and Tissue Coloration - Epon sections of 0.5 μm or more that are cut with glass knives do not have a uniform thickness as Judged by their interference colors; these colors change with time during their flotation on the knife bath, and again when drying onto the specimen support. Quoted thicknesses thus must be considered only as rough estimates unless measured in specific regions by other methods. Chloroform vapors do not always result in good spreading of thick sections; however, they will spread spontaneously to large degrees after resting on the flotation bath for several minutes. Ribbons of thick sections have been almost impossible to obtain.

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