scholarly journals A double-stranded RNA-inducible fish gene homologous to the murine influenza virus resistance gene Mx.

1989 ◽  
Vol 9 (7) ◽  
pp. 3117-3121 ◽  
Author(s):  
P Staeheli ◽  
Y X Yu ◽  
R Grob ◽  
O Haller
Keyword(s):  
Influenza Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Genomic Dna ◽  
Sequence Similarity ◽  
Perca Fluviatilis ◽  
High Sequence Similarity ◽  
Double Stranded Rna ◽  
Virus Resistance Gene

We cloned and sequenced a 2.35-kilobase EcoRI fragment of genomic DNA from a local freshwater fish (Perca fluviatilis) that strongly hybridized to probes derived from the murine influenza virus resistance gene Mx. The cloned fish DNA contained blocks of sequences related to Mx gene exons 3 to 8, which appeared to represent exons of a bona fide fish gene because they were separated by intron sequences flanked by consensus splice acceptor and donor sites. Injection of double-stranded RNA into the peritoneal cavity of trouts resulted in 5- to 10-fold elevated levels of two liver mRNAs of about 2.0 to 2.5 kilobases in length that hybridized to the cloned genomic DNA. High sequence similarity between this fish gene and the murine Mx gene, identical exon lengths, and similar inducibilities in vivo by double-stranded RNA indicate that we isolated a fragment of a fish Mx gene.

A double-stranded RNA-inducible fish gene homologous to the murine influenza virus resistance gene Mx

1989 ◽  
Vol 9 (7) ◽  
pp. 3117-3121
Author(s):  
P Staeheli ◽  
Y X Yu ◽  
R Grob ◽  
O Haller
Keyword(s):  
Influenza Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Genomic Dna ◽  
Sequence Similarity ◽  
Perca Fluviatilis ◽  
High Sequence Similarity ◽  
Double Stranded Rna ◽  
Virus Resistance Gene

We cloned and sequenced a 2.35-kilobase EcoRI fragment of genomic DNA from a local freshwater fish (Perca fluviatilis) that strongly hybridized to probes derived from the murine influenza virus resistance gene Mx. The cloned fish DNA contained blocks of sequences related to Mx gene exons 3 to 8, which appeared to represent exons of a bona fide fish gene because they were separated by intron sequences flanked by consensus splice acceptor and donor sites. Injection of double-stranded RNA into the peritoneal cavity of trouts resulted in 5- to 10-fold elevated levels of two liver mRNAs of about 2.0 to 2.5 kilobases in length that hybridized to the cloned genomic DNA. High sequence similarity between this fish gene and the murine Mx gene, identical exon lengths, and similar inducibilities in vivo by double-stranded RNA indicate that we isolated a fragment of a fish Mx gene.

scholarly journals Interferon-regulated influenza virus resistance gene Mx is localized on mouse chromosome 16.

1986 ◽  
Vol 58 (3) ◽  
pp. 967-969 ◽  
Author(s):  
P Staeheli ◽  
D Pravtcheva ◽  
L G Lundin ◽  
M Acklin ◽  
F Ruddle ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Resistance Gene ◽  
Mouse Chromosome ◽  
Virus Resistance ◽  
Chromosome 16 ◽  
Virus Resistance Gene

scholarly journals Characterization of a Polytropic Murine Leukemia Virus Proviral Sequence Associated with the Virus Resistance Gene Rmcf of DBA/2 Mice

2002 ◽  
Vol 76 (16) ◽  
pp. 8218-8224 ◽  
Author(s):  
Yong Tae Jung ◽  
Myung Soo Lyu ◽  
Alicia Buckler-White ◽  
Christine A. Kozak
Keyword(s):  
Resistance Gene ◽  
Virus Resistance ◽  
Backcross Progeny ◽  
Viral Glycoprotein ◽  
Proviral Sequence ◽  
Resistance To Infection ◽  
Murine Leukemia ◽  
Virus Resistance Gene

ABSTRACT The DBA/2 mouse Rmcf gene is responsible for in vivo and in vitro resistance to infection by the polytropic mink cell focus-forming (MCF) virus subgroup of murine leukemia viruses (MLVs). Previous studies suggested that Rmcf resistance is mediated by expression of an interfering MCF MLV envelope (Env) gene. To characterize this env gene, we examined resistance in crosses between Rmcf r DBA/2 mice and Mus castaneus, a species that lacks endogenous MCF env sequences. In backcross progeny, inheritance of Rmcf resistance correlated with inheritance of a specific endogenous MCF virus env-containing 4.6-kb EcoRI fragment. This fragment was present in the DBA/2N substrain with Rmcf-mediated resistance but not in virus-susceptible DBA/2J substrain mice. This fragment contains a provirus with a 5′ long terminal repeat and the 5′ half of env; the gag and pol genes have been partially deleted. The Env sequence is identical to that of a highly immunogenic viral glycoprotein expressed in the DBA/2 cell line L5178Y and closely resembles the env genes of modified polytropic proviruses. The coding sequence for the full-length Rmcf Env surface subunit was amplified from DNAs from virus-resistant backcross mice and was cloned into an expression vector. NIH 3T3 and BALB 3T3 cells stably transfected with this construct showed significant resistance to infection by MCF MLV but not by amphotropic MLV. This study identifies an Rmcf-linked MCF provirus and indicates that, like the ecotropic virus resistance gene Fv4, Rmcf may mediate resistance through an interference mechanism.

Cloning and functional expression of the influenza virus resistance gene MX

1985 ◽  
Vol 3 ◽  
pp. 41
Author(s):  
P. Staehell ◽  
W. Boll ◽  
J. Lindenmann ◽  
C. Weissmann ◽  
O. Haller
Keyword(s):  
Influenza Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Functional Expression ◽  
Virus Resistance Gene

Genetic mapping of the novel Turnip mosaic virus resistance gene TuRB03 in Brassica napus

2003 ◽  
Vol 107 (7) ◽  
pp. 1169-1173 ◽  
Author(s):  
S. L. Hughes ◽  
P. J. Hunter ◽  
A. G. Sharpe ◽  
M. J. Kearsey ◽  
D. J. Lydiate ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Mapping ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Turnip Mosaic Virus ◽  
The Novel ◽  
Virus Resistance Gene

scholarly journals Fine Mapping and Candidate Gene Discovery of the Soybean Mosaic Virus Resistance Gene, Rsv4

2010 ◽  
Vol 3 (1) ◽  
Author(s):  
M. A. Saghai Maroof ◽  
Dominic M. Tucker ◽  
Jeffrey A. Skoneczka ◽  
Brian C. Bowman ◽  
Sucheta Tripathy ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Fine Mapping ◽  
Virus Resistance ◽  
Soybean Mosaic Virus ◽  
Gene Discovery ◽  
Virus Resistance Gene

The tobacco mosaic virus resistance gene, N

2002 ◽  
Vol 3 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Rajendra Marathe ◽  
Radhamani Anandalakshmi ◽  
Yule Liu ◽  
S. P. Dinesh-Kumar
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Virus Resistance Gene
Sign in / Sign up

Export Citation Format

Share Document