scholarly journals Genes that modify expression of major urinary proteins in mice.

1988 ◽  
Vol 8 (7) ◽  
pp. 2705-2712 ◽  
Author(s):  
R Duncan ◽  
R Matthai ◽  
K Huppi ◽  
T Roderick ◽  
M Potter
Keyword(s):  
Restriction Fragment ◽  
Chromosomal Location ◽  
Cdna Probe ◽  
Chromosomal Marker ◽  
Chromosome 15 ◽  
Chromosome 4 ◽  
Mus Musculus Domesticus ◽  
Urinary Proteins ◽  
Major Urinary Proteins ◽  
Restriction Fragment Polymorphism

A survey of major urinary proteins (MUPs) from eight BALB/c mouse substrains by isoelectric focusing identified a common pattern with about 10 protein bands in males. One substrain, BALB/cJPt, differed in that it expressed two variant MUP patterns, designated 4.1lo and null. To find the chromosomal location of the gene which determines the 4.1lo phenotype, BALB/cJPt-MUP-4.1lo was crossed with a wild-derived Mus musculus domesticus inbred strain (CLA) that expresses the common BALB/c MUP pattern. The F1 phenotype revealed that the gene(s) controlling the MUP-4.1lo trait was recessive. A restriction fragment polymorphism between these strains found with a MUP cDNA probe allowed us to establish that a gene determining the MUP-4.1lo trait was not linked to the MUP structural genes on chromosome 4. Assays for other chromosomal marker loci revealed that a gene determining the MUP-4.1lo trait, designated Mupm-1, was closely linked to Myc-1 on chromosome 15. To determine the genetic basis of the null trait, BALB/cJPt-MUP-null mice were crossed with BALB/cJPt-MUP-4.1lo mice. A MUP restriction fragment polymorphism between these two lines was tightly linked to a gene or genes involved in determining the MUP-null phenotype. The two variant MUP phenotypes in BALB/cJ mice are determined by separate genes, one of which is located on chromosome 4 and the other on chromosome 15. The chromosomal location of Mupm-1 suggests that it produces a trans-acting factor which regulates MUP expression.

Genes that modify expression of major urinary proteins in mice

1988 ◽  
Vol 8 (7) ◽  
pp. 2705-2712
Author(s):  
R Duncan ◽  
R Matthai ◽  
K Huppi ◽  
T Roderick ◽  
M Potter
Keyword(s):  
Restriction Fragment ◽  
Chromosomal Location ◽  
Cdna Probe ◽  
Chromosomal Marker ◽  
Chromosome 15 ◽  
Chromosome 4 ◽  
Mus Musculus Domesticus ◽  
Urinary Proteins ◽  
Major Urinary Proteins ◽  
Restriction Fragment Polymorphism

A survey of major urinary proteins (MUPs) from eight BALB/c mouse substrains by isoelectric focusing identified a common pattern with about 10 protein bands in males. One substrain, BALB/cJPt, differed in that it expressed two variant MUP patterns, designated 4.1lo and null. To find the chromosomal location of the gene which determines the 4.1lo phenotype, BALB/cJPt-MUP-4.1lo was crossed with a wild-derived Mus musculus domesticus inbred strain (CLA) that expresses the common BALB/c MUP pattern. The F1 phenotype revealed that the gene(s) controlling the MUP-4.1lo trait was recessive. A restriction fragment polymorphism between these strains found with a MUP cDNA probe allowed us to establish that a gene determining the MUP-4.1lo trait was not linked to the MUP structural genes on chromosome 4. Assays for other chromosomal marker loci revealed that a gene determining the MUP-4.1lo trait, designated Mupm-1, was closely linked to Myc-1 on chromosome 15. To determine the genetic basis of the null trait, BALB/cJPt-MUP-null mice were crossed with BALB/cJPt-MUP-4.1lo mice. A MUP restriction fragment polymorphism between these two lines was tightly linked to a gene or genes involved in determining the MUP-null phenotype. The two variant MUP phenotypes in BALB/cJ mice are determined by separate genes, one of which is located on chromosome 4 and the other on chromosome 15. The chromosomal location of Mupm-1 suggests that it produces a trans-acting factor which regulates MUP expression.

scholarly journals Structural genes of the mouse major urinary protein are on chromosome 4.

1982 ◽  
Vol 94 (2) ◽  
pp. 414-417 ◽  
Author(s):  
K Krauter ◽  
L Leinwand ◽  
P D'Eustachio ◽  
F Ruddle ◽  
J E Darnell
Keyword(s):  
Protein Synthesis ◽  
Sequence Analysis ◽  
Chromosome 4 ◽  
Structural Genes ◽  
Urinary Proteins ◽  
Coding Region ◽  
Somatic Cell Genetics ◽  
Major Urinary Protein ◽  
Major Urinary Proteins ◽  
Trans Regulation

The major urinary proteins (MUPs) of mouse are a family of at least three major proteins which are synthesized in the liver of all strains of mice. The relative levels of synthesis of these proteins with respect to each other in the presence of testosterone is regulated by the Mup-a locus located on chromosome 4. In an effort to determine the mechanism of this regulation in molecular terms, a cDNA clone containing most of the coding region of a MUP protein has been isolated and identified by partial DNA sequence analysis. Using a combination of hybridization analysis and somatic cell genetics, the structural gene family has been unambiguously mapped to mouse chromosome 4. These data suggest that Mup-a regulation operates in a cis fashion and that models proposing trans regulation of MUP protein synthesis are unlikely.

scholarly journals Linkage between sperm abnormality level and major urinary protein phenotype in mice

1991 ◽  
Vol 57 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Jozefa Styrna
Keyword(s):  
Inbred Strains ◽  
Chromosome 4 ◽  
Urinary Proteins ◽  
Major Urinary Protein ◽  
Major Urinary Proteins ◽  
Abnormal Sperm ◽  
Inbred Strains Of Mice ◽  
Sperm Abnormality ◽  
High Level ◽  
Very High

SummarySegregation of sperm abnormality level and the pattern of major urinary proteins (MUPs) were investigated in F2 and B1 hybrid males obtained from crosses involving two contrasting inbred strains of mice: CBA/Kw (Mup-1a1a, 3·3% abnormal sperm) and C57BL/Kw (Mup-1b1b, 21·9% abnormal sperm). In the progeny of both crosses mean levels of abnormal spermatozoa were significantly higher for males typed as Mup-1b1b than for heterozygous Mup-1a1b males. Moreover, all F2 hybrid males showing very high percentages of abnormal sperm were Mup-1b1bhomozygotes. Similarly, among B1 males with a high level of deformed spermatozoa, a statistically significant majority were Mup-1b1b genotypes. Our results suggest that at least two genes which influence sperm abnormality level are segregating in these crosses. Both appear to be recessive for high sperm abnormality level, and one shows weak linkage to Mup-1 on chromosome 4.

Characterization and Comparison of Major Urinary Proteins from the House Mouse, Mus musculus domesticus, and the Aboriginal Mouse, Mus macedonicus

2007 ◽  
Vol 33 (3) ◽  
pp. 613-630 ◽  
Author(s):  
Duncan H. L. Robertson ◽  
Jane L. Hurst ◽  
Jeremy B. Searle ◽  
İslam Gündüz ◽  
Robert J. Beynon
Keyword(s):  
House Mouse ◽  
Mus Musculus ◽  
Mus Musculus Domesticus ◽  
Urinary Proteins ◽  
Major Urinary Proteins

scholarly journals cDNA sequence and chromosomal localization of the mouse parvalbumin gene, Pva

1989 ◽  
Vol 54 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Ch. Zühlke ◽  
F. Schöffl ◽  
H. Jockusch ◽  
D. Simon ◽  
J.-L. Guénet
Keyword(s):  
Restriction Fragment ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Single Copy ◽  
Chromosome 15 ◽  
Mus Musculus Domesticus ◽  
Mus Spretus ◽  
Protein Coding ◽  
Righting Response ◽  
Mouse Cdna

SummaryIn the homozygous condition, the mutation adr (arrested development of righting response) of the mouse causes a myotonia and a drastic reduction of the Ca2+-binding protein parvalbumin (PV) in fast muscles. Using a rat PV probe, a mouse cDNA clone was isolated from a λgt11 wild-type fast-muscle library and its nucleotide sequence was determined. The protein coding and the 3′ non-translated regions of the mouse gene show extensive homology with the rat PV gene. The result of Southern blot hybridization is consistent with a single copy gene for parvalbumin. Restriction fragment length polymorphisms (RFLPs) between Mus musculus domesticus (e.g. C57BL/6) and Mus spretus (SPE) were detected with the enzymes Eco RI, Pst I, and Sst I. The restriction fragment patterns of DNA samples from 65 individual offspring of (C57BL/6 × SPE)F1 × C57BL/6 backcrosses were tested with the PV probe and matched, for linkage detection, to pre-existing patterns established with various RFLP probes on the same samples. A co-distribution of PV-RFLPs with Pvt-1 and Mlvi-2, which had been localized on chromosome 15, was detected. Thus, the structural gene for PV, designated Pva, maps to chromosome 15 of the mouse whereas the adr mutation shows no linkage with markers on this chromosome. Gene locus homology between chromsome 15 of the mouse and chromosome 22 of man (which carries the human PV gene) is discussed.

scholarly journals Linkage analysis of the murine interferon-alpha locus on chromosome 4.

1984 ◽  
Vol 160 (1) ◽  
pp. 294-302 ◽  
Author(s):  
F Dandoy ◽  
K A Kelley ◽  
J DeMaeyer-Guignard ◽  
E DeMaeyer ◽  
P M Pitha
Keyword(s):  
Southern Blot ◽  
Blot Analysis ◽  
Southern Blot Analysis ◽  
Cdna Probe ◽  
Inbred Strains ◽  
Chromosome 4 ◽  
Strain Distribution Pattern ◽  
Ifn Alpha ◽  
Restriction Fragment Polymorphism ◽  
Histocompatibility Locus

Southern blot analysis with a murine interferon-alpha2 (MuIFN-alpha2) cDNA probe revealed restriction fragment polymorphism of EcoRI- and HindIII-digested C57BL/6 and BALB/cDNA. The inheritance pattern of this polymorphism was examined using DNA from each of the seven recombinant inbred strains derived from C57BL/6 and BALB/c; the strain distribution pattern suggests linkage of INF-alpha genes to two histocompatibility loci on chromosome 4. Southern blot analysis of DNA from six bilinear congenic strains carrying different fragments of the BALB/c chromosome 4 on a C57BL/6 background showed linkage of IFN-alpha genes to the histocompatibility locus H-15. It can therefore be concluded that the IFN-alpha gene cluster is situated on chromosome 4 near the H-15 locus, between loci Mup-1 and b.

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