scholarly journals Transfection of an immunoglobulin kappa gene into mature human B lymphocytes.

1988 ◽  
Vol 8 (1) ◽  
pp. 511-513 ◽  
Author(s):  
L T Bich-Thuy ◽  
C Queen
Keyword(s):  
B Lymphocytes ◽  
Light Chain ◽  
Interleukin 2 ◽  
Myeloma Cell ◽  
Chain Gene ◽  
Start Site ◽  
Immunoglobulin Light Chain ◽  
Base Pairs ◽  
Light Chain Gene ◽  
Immunoglobulin Kappa

We show in this report that the transcription induced by interleukin-2 or pokeweed mitogens of the kappa MOPC 41 immunoglobulin light-chain gene transfected into primary human or murine B lymphocytes initiates from a previously unobserved start site about 26 base pairs upstream of the start site used in myeloma cell lines.

Transfection of an immunoglobulin kappa gene into mature human B lymphocytes

1988 ◽  
Vol 8 (1) ◽  
pp. 511-513
Author(s):  
L T Bich-Thuy ◽  
C Queen
Keyword(s):  
B Lymphocytes ◽  
Light Chain ◽  
Interleukin 2 ◽  
Myeloma Cell ◽  
Chain Gene ◽  
Start Site ◽  
Immunoglobulin Light Chain ◽  
Base Pairs ◽  
Light Chain Gene ◽  
Immunoglobulin Kappa

We show in this report that the transcription induced by interleukin-2 or pokeweed mitogens of the kappa MOPC 41 immunoglobulin light-chain gene transfected into primary human or murine B lymphocytes initiates from a previously unobserved start site about 26 base pairs upstream of the start site used in myeloma cell lines.

Structure of extrachromosomal circular DNAs generated by immunoglobulin light chain gene rearrangements

1991 ◽  
Vol 27 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Toshiyasu Hirama ◽  
Sunao Takeshita ◽  
Yataroh Yoshida ◽  
Hideo Yamagishi
Keyword(s):  
Light Chain ◽  
Chain Gene ◽  
Gene Rearrangements ◽  
Immunoglobulin Light Chain ◽  
Light Chain Gene ◽  
Circular Dnas

Rearrangement and diversification of immunoglobulin light-chain genes in lymphoid cells transformed by reticuloendotheliosis virus

1989 ◽  
Vol 9 (11) ◽  
pp. 4970-4976
Author(s):  
J Y Zhang ◽  
W Bargmann ◽  
H R Bose
Keyword(s):  
Cell Lines ◽  
Light Chain ◽  
Lymphoid Cells ◽  
Chain Gene ◽  
Gene Rearrangements ◽  
B Cell Differentiation ◽  
Immunoglobulin Light Chain ◽  
Light Chain Gene ◽  
Transformed Cell Lines

Avian lymphoid cells transformed by reticuloendotheliosis virus (REV-T) serve as a model to analyze the mechanism by which B-cell differentiation and antibody diversification occur in birds. Immunoglobulin light-chain gene rearrangements, diversification, and expression were analyzed in 72 independently derived REV-T-transformed cell lines. Lymphoid cells transformed as the result of expression of the v-rel oncogene were divided into two distinct groups based on light-chain gene rearrangements. The status of the light-chain gene loci in these REV-T-transformed cell lines was determined in part by the ages of the chickens whose spleen cells were transformed. In embryonic spleen cell lines transformed by the v-rel oncogene, rearrangements were not detected, even after prolonged culture in vitro, indicating that these cells are arrested in B-cell differentiation. REV-T transformants derived from spleens obtained from chickens 2 weeks old or older, however, had at least one light-chain allele rearranged. All of the cell lines analyzed which exhibited rearranged light-chain genes contained light-chain transcripts, and most of the REV-T-transformed cells which displayed light-chain rearrangements expressed immunoglobulin protein. REV-T, therefore, transforms B-lymphoid cells at phenotypically different stages of development. Many REV-T-transformed cells undergo immunoglobulin chain gene rearrangements during prolonged propagation in vitro. Most of the cell lines which rearrange their light-chain alleles also undergo diversification during cultivation in vitro. Light-chain diversification occurs during or after the rearrangement event.

scholarly journals Immunoglobulin light chain gene rearrangements display hierarchy in absence of selection for functionality in precursor-B-ALL

Leukemia ◽  
2002 ◽  
Vol 16 (8) ◽  
pp. 1448-1453 ◽  
Author(s):  
M van der Burg ◽  
BH Barendregt ◽  
T Szczepañski ◽  
ER van Wering ◽  
AW Langerak ◽  
...  
Keyword(s):  
Light Chain ◽  
Chain Gene ◽  
Gene Rearrangements ◽  
Immunoglobulin Light Chain ◽  
Light Chain Gene ◽  
Selection For

Ongoing diversification of the rearranged immunoglobulin light-chain gene in a bursal lymphoma cell line

1990 ◽  
Vol 10 (6) ◽  
pp. 3224-3231
Author(s):  
S Kim ◽  
E H Humphries ◽  
L Tjoelker ◽  
L Carlson ◽  
C B Thompson
Keyword(s):  
B Cell ◽  
Light Chain ◽  
Gene Segment ◽  
B Cell Development ◽  
Bursa Of Fabricius ◽  
Chain Gene ◽  
Lymphoma Cell Line ◽  
Immunoglobulin Light Chain ◽  
Light Chain Gene

The chicken immunoglobulin light-chain gene (IgL) encodes only a single variable gene segment capable of recombination. To generate an immune repertoire, chickens diversify this unique rearranged VL gene segment during B-cell development in the bursa of Fabricius. Sequence analysis of IgL cDNAs suggests that both gene conversion events derived from VL segment pseudogene templates (psi VL) and non-template-derived single-base-pair substitutions contribute to this diversity. To facilitate the study of postrecombinational mechanisms of immunoglobulin gene diversification, avian B-cell lines were examined for the ability to diversify their rearranged IgL gene during in vitro passage. One line that retains this ability, the avian leukosis virus-induced bursal lymphoma cell line DT40, has been identified. After passage for 1 year in culture, 39 of 51 randomly sequenced rearranged V-J segments from a DT40 population defined novel subclones of the parental tumor. All cloned V-J segments displayed the same V-J joint, confirming that the observed diversity arose after V-J rearrangement. Most sequence variations that we observed (203 of 220 base pairs) appeared to result from psi VL-derived gene conversion events; 16 of the 17 novel single nucleotide substitutions were transitions. Based on these data, it appears that immunoglobulin diversification during in vitro passage of DT40 cells is representative of the diversification that occurs during normal B-cell development in the bursa of Fabricius.

scholarly journals The added value of immunoglobulin Kappa light chain gene (IGK) rearrangement analysis in suspected B-cell lymphomas: three illustrative cases

2012 ◽  
Vol 5 (1-2) ◽  
pp. 45-56 ◽  
Author(s):  
Paula Gameiro ◽  
Marta Sebastião ◽  
Signe Spetalen ◽  
Maria Gomes da Silva ◽  
José Cabeçadas
Keyword(s):  
B Cell ◽  
Light Chain ◽  
Added Value ◽  
Chain Gene ◽  
Kappa Light Chain ◽  
B Cell Lymphomas ◽  
Light Chain Gene ◽  
Immunoglobulin Kappa
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