scholarly journals Interferons increase transcription of a major histocompatibility class I gene via a 5' interferon consensus sequence.

1987 ◽  
Vol 7 (7) ◽  
pp. 2625-2630 ◽  
Author(s):  
K Sugita ◽  
J Miyazaki ◽  
E Appella ◽  
K Ozato
Keyword(s):  
Consensus Sequence ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Class I ◽  
Major Histocompatibility ◽  
Major Histocompatibility Class ◽  
Ifn Alpha ◽  
Alpha Beta ◽  
I Gene ◽  
Major Histocompatibility Class I

Interferons (IFNs) augment expression of major histocompatibility class I genes in many cells. To study the effect of IFNs on transcription of class I genes, we prepared and tested activity of chloramphenicol acetyltransferase (CAT) hybrid genes in which the cat gene is under the control of the 5' flanking region of the murine H-2Ld gene. NIH 3T3 cells transiently transfected with a cat construct having the sequence from position -210 to -134 showed a four- to fivefold increase in CAT activity when treated with IFN-alpha/beta. This sequence contains the IFN consensus sequence (ICS) shared among IFN-inducible genes, as well as the class I regulatory element (CRE) that controls up and down regulation of class I gene expression. To determine the precise sequence requirement for the IFN action, the ICS and CRE were independently placed upstream of the class I or a heterologous simian virus 40 promoter, and CAT activity was tested. The ICS, but not the CRE, enhanced activity of both promoters by about twofold upon exposure to IFN-alpha/beta, although greater responses were noted when the ICS and CRE were combined. These results demonstrate that the ICS alone is capable of enhancing promoter activity in response to IFN-alpha/beta treatment and that the CRE exerts a synergistic effect. Further, we show that the ICS functions as an inducible enhancer since it acts regardless of its orientation and distance in the simian virus 40 promoter.

Interferons increase transcription of a major histocompatibility class I gene via a 5' interferon consensus sequence

1987 ◽  
Vol 7 (7) ◽  
pp. 2625-2630
Author(s):  
K Sugita ◽  
J Miyazaki ◽  
E Appella ◽  
K Ozato
Keyword(s):  
Consensus Sequence ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Class I ◽  
Major Histocompatibility ◽  
Major Histocompatibility Class ◽  
Ifn Alpha ◽  
Alpha Beta ◽  
I Gene ◽  
Major Histocompatibility Class I

Interferons (IFNs) augment expression of major histocompatibility class I genes in many cells. To study the effect of IFNs on transcription of class I genes, we prepared and tested activity of chloramphenicol acetyltransferase (CAT) hybrid genes in which the cat gene is under the control of the 5' flanking region of the murine H-2Ld gene. NIH 3T3 cells transiently transfected with a cat construct having the sequence from position -210 to -134 showed a four- to fivefold increase in CAT activity when treated with IFN-alpha/beta. This sequence contains the IFN consensus sequence (ICS) shared among IFN-inducible genes, as well as the class I regulatory element (CRE) that controls up and down regulation of class I gene expression. To determine the precise sequence requirement for the IFN action, the ICS and CRE were independently placed upstream of the class I or a heterologous simian virus 40 promoter, and CAT activity was tested. The ICS, but not the CRE, enhanced activity of both promoters by about twofold upon exposure to IFN-alpha/beta, although greater responses were noted when the ICS and CRE were combined. These results demonstrate that the ICS alone is capable of enhancing promoter activity in response to IFN-alpha/beta treatment and that the CRE exerts a synergistic effect. Further, we show that the ICS functions as an inducible enhancer since it acts regardless of its orientation and distance in the simian virus 40 promoter.

scholarly journals Enhancement of major histocompatibility class I protein synthesis by DNA damage in cultured human fibroblasts and keratinocytes.

1989 ◽  
Vol 9 (2) ◽  
pp. 847-850 ◽  
Author(s):  
M E Lambert ◽  
Z A Ronai ◽  
I B Weinstein ◽  
J I Garrels
Keyword(s):  
Dna Damage ◽  
Protein Synthesis ◽  
Human Fibroblasts ◽  
Simian Virus 40 ◽  
Class I ◽  
Major Histocompatibility ◽  
Class I Mhc ◽  
Mhc I ◽  
Major Histocompatibility Class ◽  
Major Histocompatibility Class I

Exposure of primary human fibroblasts or simian virus 40-transformed human keratinocytes to several different classes of DNA damage, including UV light C (254 nm), resulted in a rapid increase in the expression of human major histocompatibility class I (MHC-I) proteins. MHC-I induction was also detected after exposure to low doses of the protein synthesis inhibitor cycloheximide, suggesting that MHC-I induction by DNA damage may be a component in a derepressible cellular SOS pathway.

Enhancement of major histocompatibility class I protein synthesis by DNA damage in cultured human fibroblasts and keratinocytes

1989 ◽  
Vol 9 (2) ◽  
pp. 847-850
Author(s):  
M E Lambert ◽  
Z A Ronai ◽  
I B Weinstein ◽  
J I Garrels
Keyword(s):  
Dna Damage ◽  
Protein Synthesis ◽  
Human Fibroblasts ◽  
Simian Virus 40 ◽  
Class I ◽  
Major Histocompatibility ◽  
Class I Mhc ◽  
Mhc I ◽  
Major Histocompatibility Class ◽  
Major Histocompatibility Class I

Exposure of primary human fibroblasts or simian virus 40-transformed human keratinocytes to several different classes of DNA damage, including UV light C (254 nm), resulted in a rapid increase in the expression of human major histocompatibility class I (MHC-I) proteins. MHC-I induction was also detected after exposure to low doses of the protein synthesis inhibitor cycloheximide, suggesting that MHC-I induction by DNA damage may be a component in a derepressible cellular SOS pathway.

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