scholarly journals Expression of three genes for elongation factor 1 alpha during morphogenesis of Mucor racemosus.

1987 ◽  
Vol 7 (5) ◽  
pp. 1925-1932 ◽  
Author(s):  
J E Linz ◽  
P S Sypherd
Keyword(s):  
Sequence Data ◽  
Elongation Factor ◽  
Yeast Cells ◽  
Northern Analysis ◽  
Mucor Racemosus ◽  
S1 Nuclease ◽  
Nucleotide Sequence Data ◽  
Elongation Factor 1 Alpha ◽  
Nuclease Mapping ◽  
Elongation Factor 1

Three genes, TEF-1, -2, and -3, encode elongation factor 1 alpha in Mucor racemosus. Neutral and alkaline S1 nuclease analyses revealed that the genetic organization is unique for each of the genes. The number and size of the intervening sequences vary in these closely related genes, which suggests that complex genetic rearrangements gave rise to the elongation factor 1 alpha gene family. Nucleotide sequence data from restriction fragments isolated from the 5' and 3' ends of TEF-2 and -3 confirmed the presence of a second intervening sequence in these genes. These data along with S1 nuclease mapping revealed a region at the 3' end of the three genes which was predicted to be transcribed but untranslated. Unique oligonucleotides containing 19 bases were synthesized to hybridize to this unique trailer region in the elongation factor 1 alpha transcripts. These oligonucleotides were used as probes in standard Northern analysis of RNA purified from M. racemosus cells of several morphological types. It was determined that all three genes were expressed in the cell morphological types studied. However, the accumulated level of transcript derived from each gene varied considerably, with TEF-1 mRNA present in approximately twofold greater quantity than the TEF-3 transcript and up to sixfold greater quantity than TEF-2. The level of TEF-1 and -2 mRNA varied little among the cell morphological types studied, whereas TEF-3 mRNA was present in twofold greater quantity in sporangiospores than in either germlings or yeast cells which had been induced to undergo morphogenesis to hyphae. These data suggest that there is differential expression of the genes encoding elongation factor 1 alpha in M. racemosus. At least one gene, TEF-3, shows a morphology-specific pattern of transcript accumulation.

Expression of three genes for elongation factor 1 alpha during morphogenesis of Mucor racemosus

1987 ◽  
Vol 7 (5) ◽  
pp. 1925-1932
Author(s):  
J E Linz ◽  
P S Sypherd
Keyword(s):  
Sequence Data ◽  
Elongation Factor ◽  
Yeast Cells ◽  
Northern Analysis ◽  
Mucor Racemosus ◽  
S1 Nuclease ◽  
Nucleotide Sequence Data ◽  
Elongation Factor 1 Alpha ◽  
Nuclease Mapping ◽  
Elongation Factor 1

Three genes, TEF-1, -2, and -3, encode elongation factor 1 alpha in Mucor racemosus. Neutral and alkaline S1 nuclease analyses revealed that the genetic organization is unique for each of the genes. The number and size of the intervening sequences vary in these closely related genes, which suggests that complex genetic rearrangements gave rise to the elongation factor 1 alpha gene family. Nucleotide sequence data from restriction fragments isolated from the 5' and 3' ends of TEF-2 and -3 confirmed the presence of a second intervening sequence in these genes. These data along with S1 nuclease mapping revealed a region at the 3' end of the three genes which was predicted to be transcribed but untranslated. Unique oligonucleotides containing 19 bases were synthesized to hybridize to this unique trailer region in the elongation factor 1 alpha transcripts. These oligonucleotides were used as probes in standard Northern analysis of RNA purified from M. racemosus cells of several morphological types. It was determined that all three genes were expressed in the cell morphological types studied. However, the accumulated level of transcript derived from each gene varied considerably, with TEF-1 mRNA present in approximately twofold greater quantity than the TEF-3 transcript and up to sixfold greater quantity than TEF-2. The level of TEF-1 and -2 mRNA varied little among the cell morphological types studied, whereas TEF-3 mRNA was present in twofold greater quantity in sporangiospores than in either germlings or yeast cells which had been induced to undergo morphogenesis to hyphae. These data suggest that there is differential expression of the genes encoding elongation factor 1 alpha in M. racemosus. At least one gene, TEF-3, shows a morphology-specific pattern of transcript accumulation.

Three genes for the elongation factor EF-1 alpha in Mucor racemosus

1986 ◽  
Vol 6 (2) ◽  
pp. 593-600
Author(s):  
J E Linz ◽  
C Katayama ◽  
P S Sypherd
Keyword(s):  
Nucleotide Sequence ◽  
Genomic Dna ◽  
Sequence Data ◽  
Elongation Factor ◽  
Dna Fragments ◽  
Mucor Racemosus ◽  
S1 Nuclease ◽  
Nucleotide Sequence Data ◽  
Flanking Regions ◽  
Overlapping Region

We cloned three genes from Mucor racemosus coding for protein synthesis elongation factor 1 alpha (EF-1 alpha). A 110-base-pair (bp) EF-1 alpha-specific cDNA clone was identified by hybrid-selected translation. The nucleotide sequence of the cDNA showed significant homology to a region of the Saccharomyces cerevisiae genes for EF-1 alpha (TEF1 and TEF2). The cDNA was used to isolate an 850-bp EcoRI genomic DNA fragment containing a portion of the EF-1 alpha gene. Screening of a lambda/M. racemosus genomic DNA bank with the 850-bp EcoRI probe resulted in the identification of three DNA fragments containing a common 850-bp EcoRI fragment within a short overlapping region. S1 nuclease analysis of the three EF-1 alpha DNA fragments showed that the EF-1 alpha transcript covered the short overlapping region in the clones. Restriction fragments purified from flanking regions in each clone were used to probe a HindIII digest of M. racemosus genomic DNA. Each flanking probe hybridized to one of three DNA fragments which hybridized to the 850-bp EF-1 alpha-specific probe. Nucleotide sequence data from two random "shotgun clones" of one of the three genes show good homology to two regions of S. cerevisiae TEF1. The data indicate the presence of three genes for EF-1 alpha in M. racemosus located at unique sites in the genome.

scholarly journals Three genes for the elongation factor EF-1 alpha in Mucor racemosus.

1986 ◽  
Vol 6 (2) ◽  
pp. 593-600 ◽  
Author(s):  
J E Linz ◽  
C Katayama ◽  
P S Sypherd
Keyword(s):  
Nucleotide Sequence ◽  
Genomic Dna ◽  
Sequence Data ◽  
Elongation Factor ◽  
Dna Fragments ◽  
Mucor Racemosus ◽  
S1 Nuclease ◽  
Nucleotide Sequence Data ◽  
Flanking Regions ◽  
Overlapping Region

We cloned three genes from Mucor racemosus coding for protein synthesis elongation factor 1 alpha (EF-1 alpha). A 110-base-pair (bp) EF-1 alpha-specific cDNA clone was identified by hybrid-selected translation. The nucleotide sequence of the cDNA showed significant homology to a region of the Saccharomyces cerevisiae genes for EF-1 alpha (TEF1 and TEF2). The cDNA was used to isolate an 850-bp EcoRI genomic DNA fragment containing a portion of the EF-1 alpha gene. Screening of a lambda/M. racemosus genomic DNA bank with the 850-bp EcoRI probe resulted in the identification of three DNA fragments containing a common 850-bp EcoRI fragment within a short overlapping region. S1 nuclease analysis of the three EF-1 alpha DNA fragments showed that the EF-1 alpha transcript covered the short overlapping region in the clones. Restriction fragments purified from flanking regions in each clone were used to probe a HindIII digest of M. racemosus genomic DNA. Each flanking probe hybridized to one of three DNA fragments which hybridized to the 850-bp EF-1 alpha-specific probe. Nucleotide sequence data from two random "shotgun clones" of one of the three genes show good homology to two regions of S. cerevisiae TEF1. The data indicate the presence of three genes for EF-1 alpha in M. racemosus located at unique sites in the genome.

A new genus, Perplexacara, and new generic placements of species of Australian marsh beetles (Coleoptera: Scirtidae) based on morphology and molecular genetic data

Zootaxa ◽  
2021 ◽  
Vol 4927 (4) ◽  
pp. 539-548
Author(s):  
C.H.S. WATTS ◽  
T.M. BRADFORD ◽  
S.J.B. COOPER
Keyword(s):  
New Genus ◽  
Sequence Data ◽  
Mitochondrial Gene ◽  
Molecular Genetic ◽  
Elongation Factor ◽  
New Combination ◽  
Cytochrome Oxidase Subunit ◽  
Molecular Genetic Data ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

The Australian Scirtidae species previously identified as misplaced in the widespread genus Prionocyphon Redtenbacher are revisited as well as their possible relationship with the Australian genus Macrodascillus (Lea) using sequence data from the mitochondrial gene, cytochrome oxidase subunit 1 and two nuclear genes, elongation factor 1-alpha and Topoisomerase. The study confirmed the conclusion of Cooper et al. (2014) that the species did not belong in Prionocyphon. The study also included a species from each of three possibly related genera, Chameloscyphon Watts, Daploeuros Watts and Dasyscyphon Watts. Chameloscyphon huonensis Watts, Dasyscyphon victoriaensis Watts and Daploeuros lamingtonensis Watts were recovered as separate lineages with C. huonensis linking with Das. victoriaensis and Dap. lamingtonensis isolated. The species previously included in Prionocyphon were shown to belong in two genera, Macrodascillus and a new genus Perplexacara: Perplexacara caementum (Watts) new combination, P. latusmandibulara (Watts) new combination, P. macroflavida (Watts) new combination, Macrodascillus scalaris (Lea), M. insolitus (Watts) new combination and M. lamingtonensis (Watts) new combination. 

scholarly journals Identification of the fungal endophyte of Ammophila breviligulata (American beachgrass) as Epichloë amarillans

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4300 ◽  
Author(s):  
Ian Drake ◽  
James F. White Jr ◽  
Faith C. Belanger
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Fungal Endophyte ◽  
Translation Elongation ◽  
Ammophila Breviligulata ◽  
Dna Sequence Data ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

The grass Ammophila breviligulata (American beachgrass) is known to host an endophyte of the genus Epichloë. Based on morphological characteristics it was originally identified as Acremonium typhinum var. ammophilae and is currently designated as Epichloë typhina var. ammophilae. However, the Epichloë species has not previously been identified based on DNA sequence data. Based on phylogenetic placement of beta-tubulin and translation elongation factor 1-alpha DNA sequences the endophyte is identified as a member of E. amarillans rather than E. typhina.

scholarly journals A new species of Cantharellus (Cantharellales, Basidiomycota, Fungi) from subalpine forest in Yunnan, China

Phytotaxa ◽  
2016 ◽  
Vol 252 (4) ◽  
pp. 273 ◽  
Author(s):  
SHI-CHENG SHAO ◽  
PEI-GUI LIU ◽  
XIAO-FEI TIAN ◽  
BART BUYCK ◽  
YAN-HONG GENG
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Elongation Factor ◽  
Systematic Position ◽  
Subalpine Forest ◽  
Transcription Elongation Factor ◽  
Subalpine Belt ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1 ◽  
A New Species

Cantharellus versicolor is described and illustrated as a new species based on morphological and molecular characters. The most significant features to distinguish the new species from other known Cantharellus are its extremely fleshy, turning gray after injury and with black floccose-fibrillose scales composed of thick-walled and irregular, erect hyphae on the pileus. It is described from the subalpine belt of Shangri-La, northwestern Yunnan, China. Phylogenetic analysis of the transcription elongation factor 1-alpha sequence data further support its systematic position in the subgenus Cantharellus and its description as a new species.

New species of Nectria and Thyronectria from Xinjiang, China

Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Alpha Actin ◽  
First Time ◽  
Elongation Factor 1

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.

Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 28 ◽  
Author(s):  
KAI CHEN ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Culture Characteristics ◽  
Close Relatives ◽  
Encoding Genes ◽  
Elongation Factor 1

Seven new species of the genus Trichoderma belonging to the Viride clade are described from soil samples of different regions in China, and named as T. adaptatum, T. beijingense, T. bifurcatum, T. mangshanicum, T. shaoguanicum, T. tardum and T. vulgatum. Their phylogenetic positions are determined by analyses of the combined partial sequences of translation elongation factor 1-alpha and the second largest RNA polymerase subunit encoding genes. Morphology and culture characteristics are observed, described and illustrated in detail. All the new species form green conidia, fit nicely the Viride clade, but differ obviously from any known species of the genus in sequence data, colony features, growth rates, and size of phialides and conidia. Distinctions between the new species and their close relatives are discussed.

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