scholarly journals DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.

1985 ◽  
Vol 5 (11) ◽  
pp. 3293-3296 ◽  
Author(s):  
R Ding ◽  
K Ghosh ◽  
A Eastman ◽  
E Bresnick
Keyword(s):  
Dna Repair ◽  
Mycophenolic Acid ◽  
Cho Cells ◽  
Human Liver ◽  
Repair Gene ◽  
Human Dna ◽  
Dna Repair Gene ◽  
Alu Sequence

Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.

DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine

1985 ◽  
Vol 5 (11) ◽  
pp. 3293-3296
Author(s):  
R Ding ◽  
K Ghosh ◽  
A Eastman ◽  
E Bresnick
Keyword(s):  
Dna Repair ◽  
Mycophenolic Acid ◽  
Cho Cells ◽  
Human Liver ◽  
Repair Gene ◽  
Human Dna ◽  
Dna Repair Gene ◽  
Alu Sequence

Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.

scholarly journals Molecular cloning and chromosomal localization of DNA sequences associated with a human DNA repair gene.

1985 ◽  
Vol 5 (2) ◽  
pp. 398-405 ◽  
Author(s):  
J S Rubin ◽  
V R Prideaux ◽  
H F Willard ◽  
A M Dulhanty ◽  
G F Whitmore ◽  
...  
Keyword(s):  
Dna Repair ◽  
Dna Sequences ◽  
Chromosomal Localization ◽  
Excision Repair ◽  
Repair Process ◽  
Human Cells ◽  
Gene Products ◽  
Repair Gene ◽  
Human Dna ◽  
Dna Repair Gene

The genes and gene products involved in the mammalian DNA repair processes have yet to be identified. Toward this end we made use of a number of DNA repair-proficient transformants that were generated after transfection of DNA from repair-proficient human cells into a mutant hamster line that is defective in the initial incision step of the excision repair process. In this report, biochemical evidence is presented that demonstrates that these transformants are repair proficient. In addition, we describe the molecular identification and cloning of unique DNA sequences closely associated with the transfected human DNA repair gene and demonstrate the presence of homologous DNA sequences in human cells and in the repair-proficient DNA transformants. The chromosomal location of these sequences was determined by using a panel of rodent-human somatic cell hybrids. Both unique DNA sequences were found to be on human chromosome 19.

scholarly journals Assignment of the XRCC2 human DNA repair gene to chromosome 7q36 by complementation analysis

Genomics ◽  
1995 ◽  
Vol 26 (3) ◽  
pp. 619-622 ◽  
Author(s):  
Nigel J. Jones ◽  
Ying Zhao ◽  
Michael J. Siciliano ◽  
Larry H. Thompson
Keyword(s):  
Dna Repair ◽  
Complementation Analysis ◽  
Repair Gene ◽  
Human Dna ◽  
Dna Repair Gene

scholarly journals Induction of a mutant phenotype in human repair proficient cells after overexpression of a mutated human DNA repair gene

1991 ◽  
Vol 19 (20) ◽  
pp. 5633-5637 ◽  
Author(s):  
Peter B.G.M. Belt ◽  
Michiel F. Van Oosterwijk ◽  
Hanny Odijk ◽  
Jan H.J. Hoeijmakers ◽  
Claude Backendorf
Keyword(s):  
Dna Repair ◽  
Mutant Phenotype ◽  
Repair Gene ◽  
Human Dna ◽  
Dna Repair Gene

Regional Assignment of the Human DNA Repair Gene (XRCC4L) to the 5q13-q14 Boundary by in Situ Hybridization

Genomics ◽  
1995 ◽  
Vol 27 (1) ◽  
pp. 215-217 ◽  
Author(s):  
Murray A. Stackhouse ◽  
Babetta L. Marrone ◽  
Deborah Grady ◽  
David J. Chen
Keyword(s):  
Dna Repair ◽  
In Situ Hybridization ◽  
Repair Gene ◽  
Human Dna ◽  
Dna Repair Gene ◽  
Regional Assignment

Molecular cloning of a human DNA repair gene

Nature ◽  
1984 ◽  
Vol 310 (5976) ◽  
pp. 425-429 ◽  
Author(s):  
A. Westerveld ◽  
J. H. J. Hoeijmakers ◽  
M. van Duin ◽  
J. de Wit ◽  
H. Odijk ◽  
...  
Keyword(s):  
Dna Repair ◽  
Molecular Cloning ◽  
Repair Gene ◽  
Human Dna ◽  
Dna Repair Gene
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