scholarly journals Activity of human growth hormone and related polypeptides on the adipose conversion of 3T3 cells.

1984 ◽  
Vol 4 (2) ◽  
pp. 228-231 ◽  
Author(s):  
M Morikawa ◽  
H Green ◽  
U J Lewis
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Culture System ◽  
Human Growth ◽  
3T3 Cells ◽  
Specific Assay ◽  
Established Line ◽  
Adipose Conversion ◽  
Cellular Responsiveness ◽  
Adipose Cells

A culture system is described for the study of cellular responsiveness to growth hormone. The hormone acts directly on an established line of preadipose 3T3 cells and promotes their differentiation into adipose cells. This response is the basis of a sensitive and specific assay and does not depend on the participation of an intermediate effector.

Activity of human growth hormone and related polypeptides on the adipose conversion of 3T3 cells

1984 ◽  
Vol 4 (2) ◽  
pp. 228-231
Author(s):  
M Morikawa ◽  
H Green ◽  
U J Lewis
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Culture System ◽  
Human Growth ◽  
3T3 Cells ◽  
Specific Assay ◽  
Established Line ◽  
Adipose Conversion ◽  
Cellular Responsiveness ◽  
Adipose Cells

A culture system is described for the study of cellular responsiveness to growth hormone. The hormone acts directly on an established line of preadipose 3T3 cells and promotes their differentiation into adipose cells. This response is the basis of a sensitive and specific assay and does not depend on the participation of an intermediate effector.

scholarly journals Interactions between the promoter and first intron are involved in transcriptional control of alpha 1(I) collagen gene expression.

1988 ◽  
Vol 8 (11) ◽  
pp. 4851-4857 ◽  
Author(s):  
P Bornstein ◽  
J McKay ◽  
D J Liska ◽  
S Apone ◽  
S Devarayalu
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Transcriptional Control ◽  
Rnase Protection Assay ◽  
Human Growth ◽  
Collagen Gene ◽  
Mrna Levels ◽  
3T3 Cells ◽  
Rnase Protection ◽  
First Intron

The first intron of the human collagen alpha 1(I) gene contains several positively and negatively acting elements. We have studied the transcription of collagen-human growth hormone fusion genes, containing deletions and rearrangements of collagen intronic sequences, by transient transfection of chick tendon fibroblasts and NIH 3T3 cells. In chick tendon fibroblasts, but not in 3T3 cells, inversion of intronic sequences containing a previously studied 274-base-pair segment, A274, resulted in markedly reduced human growth hormone mRNA levels as determined by an RNase protection assay. This inhibitory effect was largely alleviated when deletions were introduced in the collagen promoter of plasmids containing negatively oriented intronic sequences. Evidence for interaction of the promoter with the intronic segment, A274, was obtained by gel mobility shift assays. We suggest that promoter-intron interactions, mediated by DNA-binding proteins, regulate collagen gene transcription. Inversion of intronic segments containing critical interactive elements might then lead to an altered geometry and reduced activity of a transcriptional complex in those cells with sufficiently high levels of appropriate transcription factors. We further suggest that the deleted promoter segment plays a key role in directing DNA interactions involved in transcriptional control.

Human Growth Hormone Enhances Chondrogenesis and Osteogenesis in a Tissue Culture System of Chondroprogenitor Cells*

Endocrinology ◽  
1989 ◽  
Vol 125 (3) ◽  
pp. 1239-1245 ◽  
Author(s):  
GILA MAOR ◽  
ZEEV HOCHBERG ◽  
KLAUS VON DER MARK ◽  
DICK HEINEGARD ◽  
MICAHEL SILBERMANN
Keyword(s):  
Growth Hormone ◽  
Tissue Culture ◽  
Human Growth Hormone ◽  
Culture System ◽  
Human Growth ◽  
Tissue Culture System ◽  
Chondroprogenitor Cells

Interactions between the promoter and first intron are involved in transcriptional control of alpha 1(I) collagen gene expression

1988 ◽  
Vol 8 (11) ◽  
pp. 4851-4857
Author(s):  
P Bornstein ◽  
J McKay ◽  
D J Liska ◽  
S Apone ◽  
S Devarayalu
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Transcriptional Control ◽  
Rnase Protection Assay ◽  
Human Growth ◽  
Collagen Gene ◽  
Mrna Levels ◽  
3T3 Cells ◽  
Rnase Protection ◽  
First Intron

The first intron of the human collagen alpha 1(I) gene contains several positively and negatively acting elements. We have studied the transcription of collagen-human growth hormone fusion genes, containing deletions and rearrangements of collagen intronic sequences, by transient transfection of chick tendon fibroblasts and NIH 3T3 cells. In chick tendon fibroblasts, but not in 3T3 cells, inversion of intronic sequences containing a previously studied 274-base-pair segment, A274, resulted in markedly reduced human growth hormone mRNA levels as determined by an RNase protection assay. This inhibitory effect was largely alleviated when deletions were introduced in the collagen promoter of plasmids containing negatively oriented intronic sequences. Evidence for interaction of the promoter with the intronic segment, A274, was obtained by gel mobility shift assays. We suggest that promoter-intron interactions, mediated by DNA-binding proteins, regulate collagen gene transcription. Inversion of intronic segments containing critical interactive elements might then lead to an altered geometry and reduced activity of a transcriptional complex in those cells with sufficiently high levels of appropriate transcription factors. We further suggest that the deleted promoter segment plays a key role in directing DNA interactions involved in transcriptional control.

LIPOLYTIC ACTIVITY OF ENZYME DIGESTED HUMAN GROWTH HORMONE

1965 ◽  
Vol 49 (3_Suppl) ◽  
pp. S143
Author(s):  
Zvi Laron ◽  
Avivah Kowadlo-Silbergeld
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Lipolytic Activity ◽  
Human Growth

The minimal amino acid sequence of the insulin-potentiating fragments of human growth hormone: its mechanism of action

Diabetes ◽  
1980 ◽  
Vol 29 (10) ◽  
pp. 782-787 ◽  
Author(s):  
F. M. Ng ◽  
J. Bornstein ◽  
C. E. Pullin ◽  
J. O. Bromley ◽  
S. L. Macaulay
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Human Growth Hormone ◽  
Mechanism Of Action ◽  
Human Growth
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